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Strain Name:
C57BL/6J-MtgxR0015Btlr/Mmmh
Stock Number:
038310-MU
Citation ID:
RRID:MMRRC_038310-MU
Other Names:
R0015 (G1), C57BL/6J-MtgxR0015Btlr
Major Collection:

Strain Information

Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Lgals8
Name: lectin, galactose binding, soluble 8
Synonyms: Lgals-8, 1200015E08Rik, D13Ertd524e
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 56048
HGNC: HGNC:6569
Homologene: 31386
Ntrk1
Name: neurotrophic tyrosine kinase, receptor, type 1
Synonyms: TrkA, Tkr
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18211
HGNC: HGNC:8031
Homologene: 1898
Pcf11
Name: PCF11 cleavage and polyadenylation factor subunit
Synonyms: 5730417B17Rik, 2500001H09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74737
Homologene: 32282
Lypd1
Name: Ly6/Plaur domain containing 1
Synonyms: C530008O16Rik, 2700050C12Rik, Lynx2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72585
Homologene: 16937
Tead3
Name: TEA domain family member 3
Synonyms: ETFR-1, TEAD-3, DTEF-1, Tcf13r2, TEF-5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21678
Homologene: 81821
Cwf19l2
Name: CWF19 like cell cycle control factor 2
Synonyms: 3230401L03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244672
Homologene: 12366
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 63,721,800 bp
  • T to G, chromosome 1 at 92,983,232 bp
  • A to G, chromosome 1 at 125,910,438 bp
  • A to G, chromosome 1 at 131,097,326 bp
  • A to G, chromosome 1 at 132,303,005 bp
  • A to G, chromosome 1 at 137,928,656 bp
  • G to A, chromosome 2 at 13,001,000 bp
  • A to G, chromosome 2 at 19,296,321 bp
  • T to A, chromosome 2 at 51,925,667 bp
  • C to A, chromosome 2 at 67,510,899 bp
  • C to A, chromosome 2 at 71,214,484 bp
  • T to C, chromosome 2 at 91,630,607 bp
  • T to A, chromosome 2 at 119,116,327 bp
  • C to T, chromosome 2 at 155,622,286 bp
  • T to A, chromosome 3 at 38,982,503 bp
  • A to T, chromosome 3 at 58,846,427 bp
  • G to A, chromosome 3 at 76,322,191 bp
  • C to T, chromosome 3 at 80,707,767 bp
  • A to G, chromosome 3 at 87,791,750 bp
  • A to C, chromosome 3 at 89,662,773 bp
  • T to C, chromosome 3 at 127,555,397 bp
  • T to G, chromosome 4 at 66,266,382 bp
  • C to G, chromosome 4 at 134,116,025 bp
  • T to C, chromosome 4 at 152,139,152 bp
  • T to C, chromosome 5 at 35,022,776 bp
  • A to G, chromosome 6 at 14,717,661 bp
  • C to A, chromosome 6 at 116,328,297 bp
  • G to T, chromosome 6 at 125,001,540 bp
  • A to T, chromosome 7 at 4,477,557 bp
  • A to T, chromosome 7 at 75,125,641 bp
  • T to A, chromosome 7 at 92,658,317 bp
  • C to A, chromosome 8 at 22,535,345 bp
  • C to T, chromosome 8 at 60,534,571 bp
  • T to C, chromosome 8 at 70,140,367 bp
  • C to T, chromosome 8 at 104,140,927 bp
  • A to G, chromosome 9 at 3,454,666 bp
  • A to T, chromosome 9 at 18,484,858 bp
  • T to C, chromosome 9 at 20,668,741 bp
  • C to A, chromosome 9 at 27,051,809 bp
  • T to A, chromosome 9 at 38,047,667 bp
  • T to C, chromosome 9 at 79,651,385 bp
  • A to T, chromosome 9 at 107,544,419 bp
  • A to C, chromosome 9 at 110,767,929 bp
  • T to C, chromosome 10 at 8,330,065 bp
  • C to T, chromosome 10 at 39,075,436 bp
  • A to G, chromosome 10 at 86,843,617 bp
  • T to G, chromosome 10 at 128,209,350 bp
  • T to C, chromosome 11 at 86,249,287 bp
  • C to A, chromosome 11 at 87,657,335 bp
  • T to A, chromosome 11 at 100,578,908 bp
  • T to A, chromosome 11 at 117,721,458 bp
  • A to C, chromosome 11 at 119,441,606 bp
  • G to A, chromosome 12 at 4,195,260 bp
  • C to T, chromosome 12 at 25,095,803 bp
  • G to A, chromosome 12 at 84,441,780 bp
  • A to G, chromosome 13 at 12,447,298 bp
  • A to G, chromosome 13 at 49,208,056 bp
  • G to T, chromosome 13 at 54,575,957 bp
  • C to A, chromosome 13 at 84,222,414 bp
  • G to A, chromosome 14 at 30,114,971 bp
  • A to G, chromosome 14 at 51,910,445 bp
  • T to A, chromosome 15 at 7,188,186 bp
  • G to A, chromosome 15 at 8,186,184 bp
  • T to C, chromosome 15 at 44,673,500 bp
  • A to T, chromosome 16 at 13,887,683 bp
  • A to G, chromosome 17 at 8,977,197 bp
  • T to A, chromosome 17 at 23,401,849 bp
  • T to C, chromosome 17 at 28,341,351 bp
  • G to A, chromosome 17 at 31,386,356 bp
  • T to A, chromosome 17 at 56,618,406 bp
  • A to T, chromosome 17 at 94,876,768 bp
  • A to C, chromosome 18 at 9,316,682 bp
  • A to G, chromosome 18 at 37,962,959 bp
  • A to T, chromosome 19 at 7,285,777 bp
  • A to G, chromosome 19 at 8,793,652 bp
  • A to G, chromosome 19 at 48,029,100 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0015 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038310-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.