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Strain Name:
C57BL/6J-MtgxR0020Btlr/Mmmh
Stock Number:
038315-MU
Citation ID:
RRID:MMRRC_038315-MU
Other Names:
R0020 (G1), C57BL/6J-MtgxR0020Btlr
Major Collection:

Strain Information

Dbh
Name: dopamine beta hydroxylase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13166
HGNC: HGNC:2689
Homologene: 615
Atf2
Name: activating transcription factor 2
Synonyms: mXBP, CRE-BP, Creb2, D130078H02Rik, ATF-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11909
HGNC: HGNC:784
Homologene: 31061
Nap1l1
Name: nucleosome assembly protein 1-like 1
Synonyms: D10Ertd68e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 53605
VEGA: 10
HGNC: HGNC:7637
Homologene: 129218
Gon4l
Name: gon-4 like
Synonyms: 1500041I23Rik, 2610100B20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76022
Homologene: 13002
Tns3
Name: tensin 3
Synonyms: TEM6, F830010I22Rik, Tens1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319939
Homologene: 49713
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: E430014N10Rik, NBC3, NBCn1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680
Nphs1
Name: nephrosis 1, nephrin
Synonyms: nephrin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54631
HGNC: HGNC:7908
Homologene: 20974
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 6,264,548 bp
  • T to A, chromosome 1 at 90,811,550 bp
  • T to C, chromosome 1 at 172,475,515 bp
  • T to A, chromosome 1 at 174,050,847 bp
  • A to G, chromosome 2 at 27,170,572 bp
  • T to C, chromosome 2 at 73,846,284 bp
  • C to T, chromosome 2 at 102,642,078 bp
  • T to A, chromosome 2 at 120,065,631 bp
  • T to A, chromosome 2 at 148,771,333 bp
  • G to T, chromosome 3 at 88,858,937 bp
  • A to T, chromosome 3 at 107,195,420 bp
  • A to T, chromosome 3 at 121,731,616 bp
  • A to T, chromosome 4 at 25,200,054 bp
  • A to G, chromosome 4 at 70,488,149 bp
  • G to A, chromosome 4 at 96,307,404 bp
  • G to C, chromosome 4 at 96,769,735 bp
  • G to A, chromosome 4 at 149,634,796 bp
  • A to G, chromosome 5 at 14,669,673 bp
  • A to G, chromosome 5 at 104,503,516 bp
  • C to T, chromosome 5 at 137,653,802 bp
  • A to G, chromosome 5 at 149,209,779 bp
  • T to G, chromosome 6 at 47,880,009 bp
  • C to A, chromosome 6 at 48,064,707 bp
  • C to A, chromosome 6 at 61,313,804 bp
  • T to A, chromosome 6 at 65,084,007 bp
  • T to A, chromosome 6 at 137,443,594 bp
  • A to T, chromosome 6 at 141,972,350 bp
  • A to T, chromosome 7 at 26,450,372 bp
  • G to T, chromosome 7 at 30,463,208 bp
  • T to C, chromosome 7 at 45,488,104 bp
  • G to T, chromosome 7 at 105,405,677 bp
  • A to G, chromosome 7 at 109,830,888 bp
  • G to A, chromosome 7 at 127,193,824 bp
  • C to G, chromosome 7 at 127,611,169 bp
  • C to T, chromosome 8 at 71,716,870 bp
  • C to A, chromosome 9 at 110,828,512 bp
  • T to C, chromosome 10 at 67,548,097 bp
  • T to A, chromosome 10 at 78,427,336 bp
  • T to C, chromosome 10 at 85,907,623 bp
  • A to C, chromosome 10 at 111,491,023 bp
  • A to C, chromosome 11 at 8,545,227 bp
  • A to G, chromosome 11 at 8,875,765 bp
  • T to A, chromosome 11 at 58,050,023 bp
  • A to G, chromosome 11 at 60,370,545 bp
  • T to C, chromosome 11 at 103,227,674 bp
  • A to T, chromosome 12 at 32,841,013 bp
  • C to T, chromosome 12 at 110,574,823 bp
  • T to C, chromosome 12 at 114,416,621 bp
  • A to C, chromosome 13 at 16,026,364 bp
  • A to T, chromosome 13 at 62,854,048 bp
  • T to A, chromosome 13 at 109,954,570 bp
  • T to A, chromosome 14 at 14,796,108 bp
  • T to C, chromosome 14 at 69,660,793 bp
  • A to T, chromosome 14 at 78,518,177 bp
  • T to C, chromosome 15 at 3,991,868 bp
  • A to G, chromosome 15 at 44,556,872 bp
  • T to A, chromosome 15 at 89,259,139 bp
  • T to C, chromosome 15 at 101,230,552 bp
  • A to G, chromosome 16 at 5,037,049 bp
  • A to G, chromosome 16 at 22,997,296 bp
  • A to T, chromosome 16 at 49,001,612 bp
  • G to T, chromosome 17 at 6,742,727 bp
  • T to A, chromosome 17 at 45,566,856 bp
  • T to A, chromosome 17 at 55,653,429 bp
  • G to T, chromosome 18 at 57,287,893 bp
  • G to T, chromosome 18 at 58,105,164 bp
  • A to T, chromosome 19 at 29,716,197 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0020 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038315-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.