Strain Name:
C57BL/6J-MtgxR0021Btlr/Mmmh
Stock Number:
038316-MU
Citation ID:
RRID:MMRRC_038316-MU
Other Names:
R0021 (G1), C57BL/6J-MtgxR0021Btlr
Major Collection:

Gene Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: Oct-1C, Oct-1B, Oct-1A, oct-1, Otf-1, Otf1, 2810482H01Rik, Oct-1z, Oct1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330286
Homologene: 19782
Ptprk
Name: protein tyrosine phosphatase, receptor type, K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Mef2c
Name: myocyte enhancer factor 2C
Synonyms: 9930028G15Rik, 5430401D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17260
HGNC: HGNC:6996
Homologene: 31087
Map2k4
Name: mitogen-activated protein kinase kinase 4
Synonyms: MKK4, JNKK1, Serk1, Sek1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 26398
HGNC: HGNC:6844
Homologene: 48159
Dhx15
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 15
Synonyms: mDEAH9, DBP1, HRH2, Ddx15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13204
HGNC: HGNC:2738
Homologene: 1040
Serpini1
Name: serine (or cysteine) peptidase inhibitor, clade I, member 1
Synonyms: Neuroserpin, PI12, Spi17, Ns
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20713
HGNC: HGNC:8943
Homologene: 21045
Wrap53
Name: WD repeat containing, antisense to Trp53
Synonyms: Wdr79
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216853
Homologene: 9995
Nkapd1
Name: NKAP domain containing 1
Synonyms: LOC270156, AU019823
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270156
Homologene: 41233
Tbc1d10a
Name: TBC1 domain family, member 10a
Synonyms: EPI64, Tbc1d10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103724
Homologene: 32762
Abcc5
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
Synonyms: Mrp5, Abcc5b, Abcc5a, 2900011L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 27416
HGNC: HGNC:56
Homologene: 21164
Unc5b
Name: unc-5 netrin receptor B
Synonyms: 6330415E02Rik, Unc5h2, D10Bwg0792e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 107449
VEGA: 10
Homologene: 32538
Mcm9
Name: minichromosome maintenance 9 homologous recombination repair factor
Synonyms: 9030408O17Rik, Mcmdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71567
VEGA: 10
Homologene: 13546
Pdgfrb
Name: platelet derived growth factor receptor, beta polypeptide
Synonyms: CD140b, Pdgfr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 18596
HGNC: HGNC:8804
Homologene: 1960
Setd6
Name: SET domain containing 6
Synonyms: 0610039J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66083
Homologene: 38743
Saal1
Name: serum amyloid A-like 1
Synonyms: 5031425D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78935
Homologene: 34706
Clstn1
Name: calsyntenin 1
Synonyms: calsyntenin-1, Cst-1, 1810034E21Rik, alcadein alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 65945
Homologene: 8814
Baiap3
Name: BAI1-associated protein 3
Synonyms: LOC381076
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 545192
HGNC: HGNC:948
Homologene: 20844
Dock8
Name: dedicator of cytokinesis 8
Synonyms: 1200017A24Rik, 5830472H07Rik, A130095G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 76088
VEGA: 19
Homologene: 52414
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Col1a-2, Cola-2, Cola2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Trim55
Name: tripartite motif-containing 55
Synonyms: D830041C10Rik, Murf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 381485
Homologene: 13205
Dhx36
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 36
Synonyms: Ddx36, 2810407E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72162
Homologene: 6356
Mcc
Name: mutated in colorectal cancers
Synonyms: D18Ertd451e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 328949
HGNC: HGNC:6935
Homologene: 20539
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241516
Homologene: 110349
Brinp3
Name: bone morphogenetic protein/retinoic acid inducible neural specific 3
Synonyms: B830045N13Rik, Fam5c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 215378
Homologene: 17786
Scn2a
Name: sodium channel, voltage-gated, type II, alpha
Synonyms: Nav1.2, A230052E19Rik, Scn2a1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 110876
Homologene: 75001
Slc27a2
Name: solute carrier family 27 (fatty acid transporter), member 2
Synonyms: FATP2, VLCS, FATP2, Vlacs, Vlac, ACSVL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 26458
Homologene: 37830
Siah2
Name: siah E3 ubiquitin protein ligase 2
Synonyms: Sinh2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20439
Homologene: 21053
Trim45
Name: tripartite motif-containing 45
Synonyms: 4921530N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229644
Homologene: 11865
Lrrc7
Name: leucine rich repeat containing 7
Synonyms: densin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242274
Homologene: 10817
Galnt11
Name: polypeptide N-acetylgalactosaminyltransferase 11
Synonyms: E430002F06Rik, A430075I06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231050
Homologene: 11126
Krtcap3
Name: keratinocyte associated protein 3
Synonyms: 2010001C09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69815
Homologene: 12332
Plac8
Name: placenta-specific 8
Synonyms: onzin, C15, D5Wsu111e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231507
Homologene: 32328
C5ar2
Name: complement component 5a receptor 2
Synonyms: C5L2, E030029A11Rik, Gpr77
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 319430
HGNC: HGNC:4527
Homologene: 49549
V1rd19
Name: vomeronasal 1 receptor, D19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404287
Homologene: 104166
Zfp790
Name: zinc finger protein 790
Synonyms: 6330581L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233056
Homologene: 51695
Aplp1
Name: amyloid beta (A4) precursor-like protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11803
HGNC: HGNC:597
Homologene: 68447
2310016G11Rik
Name: RIKEN cDNA 2310016G11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69578
Scart2
Name: scavenger receptor family member expressed on T cells 2
Synonyms: 5830411N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244234
Homologene: 133218
Impg1
Name: interphotoreceptor matrix proteoglycan 1
Synonyms: IMP150, SPACR, A930015H12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 63859
HGNC: HGNC:6055
Homologene: 1201
Gm4981
Name: predicted gene 4981
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 245263
Homologene: 134545
Gm5134
Name: predicted gene 5134
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 333669
Homologene: 129891
Arhgef25
Name: Rho guanine nucleotide exchange factor (GEF) 25
Synonyms: 2410008H17Rik, GEFT, D10Ertd610e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 52666
VEGA: 10
Homologene: 32691
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: D11Ertd686e, Dnahc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Itgb4
Name: integrin beta 4
Synonyms: CD104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192897
HGNC: HGNC:6158
Homologene: 179
Nqo2
Name: N-ribosyldihydronicotinamide quinone reductase 2
Synonyms: NRH: quinone oxidoreductase, Ox-2, Ox2, Nmor2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18105
VEGA: 13
HGNC: HGNC:7856
Homologene: 696
Phf7
Name: PHD finger protein 7
Synonyms: 1700010P14Rik, 1700006H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71838
VEGA: 14
Homologene: 69217
Prss52
Name: protease, serine 52
Synonyms: 1700049K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 73382
Homologene: 105740
Ccr6
Name: chemokine (C-C motif) receptor 6
Synonyms: Cmkbr6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12458
VEGA: 17
HGNC: HGNC:1607
Homologene: 3214
Spaca6
Name: sperm acrosome associated 6
Synonyms: B230206P06Rik, 4930546H06Rik, Ncrna00085
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 75202
Homologene: 53443
BC003965
Name: cDNA sequence BC003965
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 214489
VEGA: 17
Homologene: 52829
Psmb9
Name: proteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2)
Synonyms: Lmp-2, Lmp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16912
HGNC: HGNC:9546
Homologene: 2094
Btnl1
Name: butyrophilin-like 1
Synonyms: NG10, LOC240074, LOC240074, Btnl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 100038862
Homologene: 103816
Hdhd2
Name: haloacid dehalogenase-like hydrolase domain containing 2
Synonyms: 0610039H12Rik, 3110052N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 76987
Homologene: 12667
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 146,901,451 bp
  • G to A, chromosome 1 at 165,876,018 bp
  • T to C, chromosome 2 at 65,670,515 bp
  • T to A, chromosome 2 at 82,999,857 bp
  • T to C, chromosome 2 at 126,567,886 bp
  • A to C, chromosome 3 at 19,644,702 bp
  • T to C, chromosome 3 at 58,676,292 bp
  • T to C, chromosome 3 at 62,477,595 bp
  • T to C, chromosome 3 at 75,619,313 bp
  • A to T, chromosome 3 at 100,925,420 bp
  • A to G, chromosome 3 at 158,160,661 bp
  • T to C, chromosome 4 at 123,475,577 bp
  • G to A, chromosome 4 at 149,634,796 bp
  • A to T, chromosome 5 at 25,248,857 bp
  • A to G, chromosome 5 at 31,252,959 bp
  • T to C, chromosome 5 at 52,157,488 bp
  • T to A, chromosome 5 at 100,556,568 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • G to A, chromosome 6 at 38,183,967 bp
  • A to G, chromosome 7 at 16,237,676 bp
  • A to T, chromosome 7 at 24,003,604 bp
  • G to A, chromosome 7 at 29,825,688 bp
  • A to G, chromosome 7 at 30,435,816 bp
  • A to G, chromosome 7 at 44,677,196 bp
  • A to T, chromosome 7 at 46,692,892 bp
  • G to A, chromosome 7 at 140,296,397 bp
  • A to G, chromosome 8 at 95,716,665 bp
  • A to C, chromosome 9 at 50,610,425 bp
  • A to T, chromosome 9 at 80,435,426 bp
  • T to A, chromosome 10 at 28,592,895 bp
  • G to A, chromosome 10 at 53,537,901 bp
  • C to A, chromosome 10 at 58,235,563 bp
  • T to C, chromosome 10 at 60,778,919 bp
  • T to A, chromosome 10 at 75,993,884 bp
  • A to G, chromosome 10 at 127,189,554 bp
  • T to C, chromosome 11 at 4,213,680 bp
  • A to G, chromosome 11 at 65,712,284 bp
  • A to G, chromosome 11 at 65,969,979 bp
  • A to T, chromosome 11 at 69,563,886 bp
  • A to T, chromosome 11 at 115,979,627 bp
  • T to C, chromosome 13 at 33,981,507 bp
  • C to A, chromosome 13 at 83,656,240 bp
  • C to T, chromosome 14 at 31,238,486 bp
  • T to G, chromosome 14 at 64,104,408 bp
  • T to A, chromosome 16 at 20,378,661 bp
  • G to A, chromosome 17 at 8,256,766 bp
  • T to A, chromosome 17 at 17,838,236 bp
  • A to G, chromosome 17 at 25,184,983 bp
  • T to C, chromosome 17 at 25,243,669 bp
  • G to A, chromosome 17 at 34,184,303 bp
  • A to G, chromosome 17 at 34,379,494 bp
  • C to G, chromosome 18 at 44,519,516 bp
  • T to A, chromosome 18 at 61,064,926 bp
  • A to T, chromosome 18 at 76,970,615 bp
  • T to C, chromosome 19 at 25,163,047 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0021 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038316-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.