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Strain Name:
C57BL/6J-MtgxR0021Btlr/Mmmh
Stock Number:
038316-MU
Citation ID:
RRID:MMRRC_038316-MU
Other Names:
R0021 (G1), C57BL/6J-MtgxR0021Btlr
Major Collection:

Strain Information

Pou2f1
Name: POU domain, class 2, transcription factor 1
Synonyms: Oct-1C, Oct-1B, Oct-1A, oct-1, Otf-1, Otf1, 2810482H01Rik, Oct-1z, Oct1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18986
HGNC: HGNC:9212
Homologene: 37658
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Mef2c
Name: myocyte enhancer factor 2C
Synonyms: 9930028G15Rik, 5430401D19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17260
HGNC: HGNC:6996
Homologene: 31087
Map2k4
Name: mitogen-activated protein kinase kinase 4
Synonyms: MKK4, JNKK1, Serk1, Sek1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26398
HGNC: HGNC:6844
Homologene: 48159
Dhx15
Name: DEAH-box helicase 15
Synonyms: mDEAH9, DBP1, HRH2, Ddx15, DEAH (Asp-Glu-Ala-His) box polypeptide 15
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13204
HGNC: HGNC:2738
Homologene: 1040
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 146,901,451 bp
  • G to A, chromosome 1 at 165,876,018 bp
  • T to C, chromosome 2 at 65,670,515 bp
  • T to A, chromosome 2 at 82,999,857 bp
  • T to C, chromosome 2 at 126,567,886 bp
  • A to C, chromosome 3 at 19,644,702 bp
  • T to C, chromosome 3 at 58,676,292 bp
  • T to C, chromosome 3 at 62,477,595 bp
  • T to C, chromosome 3 at 75,619,313 bp
  • A to T, chromosome 3 at 100,925,420 bp
  • A to G, chromosome 3 at 158,160,661 bp
  • T to C, chromosome 4 at 123,475,577 bp
  • G to A, chromosome 4 at 149,634,796 bp
  • A to T, chromosome 5 at 25,248,857 bp
  • A to G, chromosome 5 at 31,252,959 bp
  • T to C, chromosome 5 at 52,157,488 bp
  • T to A, chromosome 5 at 100,556,568 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • G to A, chromosome 6 at 38,183,967 bp
  • A to G, chromosome 7 at 16,237,676 bp
  • A to T, chromosome 7 at 24,003,604 bp
  • G to A, chromosome 7 at 29,825,688 bp
  • A to G, chromosome 7 at 30,435,816 bp
  • A to G, chromosome 7 at 44,677,196 bp
  • A to T, chromosome 7 at 46,692,892 bp
  • G to A, chromosome 7 at 140,296,397 bp
  • A to G, chromosome 8 at 95,716,665 bp
  • A to C, chromosome 9 at 50,610,425 bp
  • A to T, chromosome 9 at 80,435,426 bp
  • T to A, chromosome 10 at 28,592,895 bp
  • G to A, chromosome 10 at 53,537,901 bp
  • C to A, chromosome 10 at 58,235,563 bp
  • T to C, chromosome 10 at 60,778,919 bp
  • T to A, chromosome 10 at 75,993,884 bp
  • A to G, chromosome 10 at 127,189,554 bp
  • T to C, chromosome 11 at 4,213,680 bp
  • A to G, chromosome 11 at 65,712,284 bp
  • A to G, chromosome 11 at 65,969,979 bp
  • A to T, chromosome 11 at 69,563,886 bp
  • A to T, chromosome 11 at 115,979,627 bp
  • T to C, chromosome 13 at 33,981,507 bp
  • C to A, chromosome 13 at 83,656,240 bp
  • C to T, chromosome 14 at 31,238,486 bp
  • T to G, chromosome 14 at 64,104,408 bp
  • T to A, chromosome 16 at 20,378,661 bp
  • G to A, chromosome 17 at 8,256,766 bp
  • T to A, chromosome 17 at 17,838,236 bp
  • A to G, chromosome 17 at 25,184,983 bp
  • T to C, chromosome 17 at 25,243,669 bp
  • G to A, chromosome 17 at 34,184,303 bp
  • A to G, chromosome 17 at 34,379,494 bp
  • C to G, chromosome 18 at 44,519,516 bp
  • T to A, chromosome 18 at 61,064,926 bp
  • A to T, chromosome 18 at 76,970,615 bp
  • T to C, chromosome 19 at 25,163,047 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0021 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038316-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.