MMRRC:038332-MU

Synonyms: eIF-2 alpha, Pkr, dsRNA-activated kinase, IFN- type I-induced and dsRNA-activated kinase, IFN-induced and double-stranded RNA-activated kinase, Prkr, Tik, eIF-2 alpha, 4732414G15Rik, 2310047A08Rik

Type: Gene

Species: Mus musculus (mouse)

Chromosome: 17

MGI: MGI:1353449

NCBI: 19106

Ensembl: ENSMUSG00000024079

VEGA: OTTMUSG00000036019

HGNC: HGNC:9437

Homologene: 48134

CCDS: CCDS28980.1

UCSC: Chr17:79157933-79190001

Genetic Alterations

ENU-induced transitions at the following base pair locations (GRCm38):

A to T, chromosome 1 at 24,559,744 bp
T to C, chromosome 1 at 82,886,102 bp
G to T, chromosome 1 at 188,626,612 bp
A to T, chromosome 2 at 20,743,574 bp
T to C, chromosome 2 at 25,297,459 bp
T to A, chromosome 2 at 120,695,832 bp
A to G, chromosome 2 at 126,795,468 bp
C to T, chromosome 2 at 129,115,668 bp
T to C, chromosome 2 at 155,502,781 bp
T to C, chromosome 3 at 41,629,508 bp
T to C, chromosome 3 at 79,069,396 bp
T to C, chromosome 4 at 143,438,944 bp
G to A, chromosome 4 at 149,634,796 bp
T to C, chromosome 5 at 29,205,654 bp
A to G, chromosome 5 at 32,868,853 bp
G to A, chromosome 5 at 77,006,475 bp
T to G, chromosome 5 at 106,635,043 bp
T to A, chromosome 5 at 109,293,144 bp
T to A, chromosome 5 at 138,301,036 bp
G to A, chromosome 6 at 4,518,822 bp
A to G, chromosome 6 at 73,260,503 bp
A to G, chromosome 7 at 3,616,073 bp
T to A, chromosome 7 at 25,287,140 bp
C to A, chromosome 7 at 25,287,141 bp
G to A, chromosome 7 at 25,914,862 bp
A to G, chromosome 7 at 44,306,482 bp
T to C, chromosome 7 at 83,636,743 bp
T to C, chromosome 8 at 83,722,498 bp
T to C, chromosome 9 at 15,915,010 bp
G to A, chromosome 9 at 22,504,094 bp
T to A, chromosome 9 at 34,911,770 bp
G to A, chromosome 9 at 40,317,526 bp
A to T, chromosome 9 at 76,259,681 bp
A to T, chromosome 9 at 86,564,673 bp
A to T, chromosome 9 at 108,572,969 bp
A to T, chromosome 9 at 109,338,540 bp
T to C, chromosome 10 at 26,986,797 bp
A to T, chromosome 10 at 74,643,440 bp
T to C, chromosome 10 at 127,186,865 bp
C to T, chromosome 10 at 128,682,903 bp
A to C, chromosome 11 at 5,207,703 bp
A to G, chromosome 11 at 62,392,551 bp
A to G, chromosome 11 at 71,172,171 bp
G to A, chromosome 11 at 99,948,602 bp
T to G, chromosome 12 at 69,596,693 bp
C to G, chromosome 12 at 84,529,725 bp
A to G, chromosome 12 at 104,037,957 bp
T to C, chromosome 12 at 112,774,462 bp
T to A, chromosome 14 at 12,213,710 bp
A to T, chromosome 14 at 31,708,035 bp
T to C, chromosome 14 at 70,568,085 bp
T to G, chromosome 14 at 74,706,247 bp
A to G, chromosome 15 at 84,122,513 bp
A to T, chromosome 15 at 85,929,419 bp
T to C, chromosome 16 at 32,298,995 bp
C to A, chromosome 16 at 33,045,642 bp
T to C, chromosome 16 at 49,071,141 bp
A to G, chromosome 16 at 88,563,034 bp
T to A, chromosome 17 at 78,863,955 bp
A to G, chromosome 19 at 3,262,097 bp
A to G, chromosome 19 at 59,299,596 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Suggested Control Mice

Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R0038 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Request Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
038332-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Request Information Expand All