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Strain Name:
C57BL/6J-MtgxR0052Btlr/Mmmh
Stock Number:
038346-MU
Citation ID:
RRID:MMRRC_038346-MU
Other Names:
R0052 (G1), C57BL/6J-MtgxR0052Btlr
Major Collection:

Strain Information

Kcnj10
Name: potassium inwardly-rectifying channel, subfamily J, member 10
Synonyms: Kir1.2, BIRK-1, BIR10, Kir4.1, Kir4.1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16513
HGNC: HGNC:6256
Homologene: 1689
Rnf111
Name: ring finger 111
Synonyms: Arkadia
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 93836
VEGA: 9
Homologene: 9741
Elp3
Name: elongator acetyltransferase complex subunit 3
Synonyms: 2610507P14Rik, KAT9
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 74195
VEGA: 14
Homologene: 7105
Phf3
Name: PHD finger protein 3
Synonyms: 2310061N19Rik, AU020177
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 213109
HGNC: HGNC:8921
Homologene: 9040
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, C030045D06Rik, Depdc2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109294
Homologene: 23523
Ubap2l
Name: ubiquitin-associated protein 2-like
Synonyms: 3110083O19Rik, NICE-4, 4932431F02Rik, A430103N23Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74383
Homologene: 136291
Thada
Name: thyroid adenoma associated
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240174
VEGA: 17
Homologene: 75175
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 11,160,156 bp
  • T to C, chromosome 1 at 30,808,767 bp
  • T to A, chromosome 1 at 60,228,612 bp
  • G to A, chromosome 1 at 150,677,406 bp
  • A to G, chromosome 1 at 172,368,924 bp
  • T to A, chromosome 2 at 28,544,388 bp
  • T to C, chromosome 2 at 52,273,980 bp
  • T to A, chromosome 2 at 104,929,570 bp
  • G to A, chromosome 2 at 127,809,039 bp
  • A to T, chromosome 2 at 144,259,192 bp
  • T to A, chromosome 2 at 147,040,965 bp
  • C to T, chromosome 2 at 168,231,682 bp
  • T to A, chromosome 3 at 81,967,025 bp
  • A to T, chromosome 3 at 87,256,778 bp
  • T to C, chromosome 3 at 90,038,928 bp
  • G to A, chromosome 4 at 55,011,762 bp
  • A to T, chromosome 4 at 156,218,115 bp
  • A to T, chromosome 5 at 21,375,349 bp
  • A to C, chromosome 5 at 89,156,336 bp
  • T to C, chromosome 5 at 123,046,663 bp
  • A to G, chromosome 5 at 146,739,104 bp
  • T to C, chromosome 6 at 41,113,916 bp
  • T to C, chromosome 6 at 90,036,264 bp
  • T to A, chromosome 6 at 124,062,033 bp
  • C to T, chromosome 7 at 10,580,400 bp
  • T to A, chromosome 7 at 10,725,962 bp
  • T to C, chromosome 7 at 14,465,208 bp
  • G to A, chromosome 7 at 24,612,579 bp
  • A to G, chromosome 7 at 29,725,020 bp
  • G to T, chromosome 7 at 30,038,328 bp
  • G to T, chromosome 7 at 73,943,290 bp
  • A to T, chromosome 7 at 73,971,952 bp
  • A to T, chromosome 7 at 74,504,326 bp
  • A to G, chromosome 7 at 109,279,669 bp
  • A to T, chromosome 7 at 125,667,971 bp
  • A to G, chromosome 7 at 126,457,897 bp
  • A to G, chromosome 8 at 67,882,979 bp
  • A to T, chromosome 8 at 84,676,225 bp
  • A to T, chromosome 9 at 50,605,030 bp
  • G to T, chromosome 9 at 66,400,156 bp
  • A to T, chromosome 9 at 70,476,389 bp
  • A to G, chromosome 9 at 75,018,983 bp
  • T to A, chromosome 9 at 118,636,549 bp
  • T to A, chromosome 10 at 7,779,306 bp
  • C to T, chromosome 10 at 60,358,082 bp
  • C to CN, chromosome 10 at 81,178,768 bp
  • T to C, chromosome 10 at 121,800,827 bp
  • C to T, chromosome 11 at 59,158,901 bp
  • C to T, chromosome 11 at 59,565,128 bp
  • C to T, chromosome 11 at 90,259,998 bp
  • T to C, chromosome 11 at 108,949,270 bp
  • T to A, chromosome 12 at 112,767,857 bp
  • C to T, chromosome 13 at 23,855,858 bp
  • A to G, chromosome 13 at 38,197,364 bp
  • T to A, chromosome 13 at 47,064,117 bp
  • T to A, chromosome 13 at 49,375,314 bp
  • A to G, chromosome 14 at 51,418,700 bp
  • A to G, chromosome 14 at 65,531,526 bp
  • T to C, chromosome 15 at 90,970,857 bp
  • A to G, chromosome 16 at 34,357,171 bp
  • A to G, chromosome 16 at 45,606,856 bp
  • A to T, chromosome 16 at 59,565,656 bp
  • G to T, chromosome 17 at 19,811,641 bp
  • A to G, chromosome 17 at 79,650,331 bp
  • A to T, chromosome 17 at 84,455,158 bp
  • A to T, chromosome 18 at 82,639,332 bp
  • G to A, chromosome 18 at 84,014,945 bp
  • T to C, chromosome 19 at 23,915,951 bp
  • A to G, chromosome 19 at 58,968,553 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0052 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038346-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.