Strain Name:
C57BL/6J-MtgxR0054Btlr/Mmmh
Stock Number:
038348-MU
Citation ID:
RRID:MMRRC_038348-MU
Other Names:
R0054 (G1), C57BL/6J-MtgxR0054Btlr
Major Collection:

Gene Information

Gene Symbol: Ptprz1 [MGI:97816] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, Ptpz, phosphacan, Rptpbeta, Ptprz, RPTPz, PTPbeta, PTPzeta
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Sez6 [MGI:104745] (Mus musculus (mouse))
Name: seizure related gene 6
Synonyms: D11Bhm177e, sez-6
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Ncoa3 [MGI:1276535] (Mus musculus (mouse))
Name: nuclear receptor coactivator 3
Synonyms: pCIP, RAC3, KAT13B, AIB1, 2010305B15Rik, Src3, TRAM1, TRAM-1, bHLHe42
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Fam53a [MGI:1919225] (Mus musculus (mouse))
Name: family with sequence similarity 53, member A
Synonyms: DNTNP, 5430419M09Rik, 2410018C17Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Tmem87b [MGI:1919727] (Mus musculus (mouse))
Name: transmembrane protein 87B
Synonyms: 2610301K12Rik, 2810431I02Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Dido1 [MGI:1344352] (Mus musculus (mouse))
Name: death inducer-obliterator 1
Synonyms: Datf1, 6720461J16Rik, D130048F08Rik, dido, DIO-1
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Gphn [MGI:109602] (Mus musculus (mouse))
Name: gephyrin
Synonyms: geph, 5730552E08Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 18,251,247 bp
  • T to A, chromosome 1 at 60,287,401 bp
  • T to C, chromosome 1 at 75,184,499 bp
  • T to A, chromosome 1 at 78,462,374 bp
  • T to C, chromosome 1 at 79,779,495 bp
  • T to C, chromosome 1 at 87,099,765 bp
  • G to A, chromosome 1 at 185,341,216 bp
  • T to C, chromosome 1 at 191,082,184 bp
  • G to A, chromosome 2 at 9,858,447 bp
  • A to G, chromosome 2 at 40,742,817 bp
  • C to T, chromosome 2 at 59,932,166 bp
  • A to T, chromosome 2 at 76,796,460 bp
  • T to A, chromosome 2 at 82,976,608 bp
  • A to C, chromosome 2 at 82,986,955 bp
  • T to C, chromosome 2 at 86,085,361 bp
  • G to T, chromosome 2 at 91,234,820 bp
  • G to A, chromosome 2 at 111,408,795 bp
  • A to G, chromosome 2 at 116,887,287 bp
  • T to A, chromosome 2 at 128,831,441 bp
  • A to G, chromosome 2 at 166,055,178 bp
  • T to C, chromosome 2 at 180,661,474 bp
  • A to G, chromosome 3 at 28,095,884 bp
  • A to T, chromosome 3 at 94,762,592 bp
  • A to G, chromosome 4 at 11,204,595 bp
  • A to G, chromosome 4 at 11,785,186 bp
  • A to T, chromosome 4 at 25,269,087 bp
  • T to C, chromosome 4 at 44,988,915 bp
  • T to A, chromosome 4 at 45,890,900 bp
  • A to G, chromosome 4 at 75,278,100 bp
  • T to C, chromosome 4 at 112,645,463 bp
  • T to A, chromosome 4 at 125,072,840 bp
  • C to A, chromosome 4 at 125,623,575 bp
  • T to C, chromosome 4 at 129,657,458 bp
  • T to A, chromosome 4 at 143,795,002 bp
  • T to A, chromosome 5 at 21,250,935 bp
  • C to A, chromosome 5 at 33,607,732 bp
  • T to G, chromosome 5 at 69,559,561 bp
  • A to C, chromosome 5 at 89,156,336 bp
  • T to A, chromosome 5 at 108,745,664 bp
  • T to A, chromosome 6 at 22,986,196 bp
  • A to G, chromosome 6 at 53,447,657 bp
  • A to G, chromosome 6 at 82,919,693 bp
  • C to T, chromosome 6 at 83,652,929 bp
  • T to A, chromosome 6 at 123,853,025 bp
  • A to G, chromosome 6 at 142,601,774 bp
  • T to G, chromosome 6 at 146,829,718 bp
  • T to G, chromosome 7 at 23,504,909 bp
  • T to A, chromosome 7 at 44,734,475 bp
  • T to C, chromosome 7 at 98,065,698 bp
  • T to C, chromosome 8 at 65,001,321 bp
  • T to C, chromosome 8 at 72,492,571 bp
  • G to A, chromosome 9 at 21,275,365 bp
  • G to A, chromosome 9 at 21,774,674 bp
  • A to T, chromosome 9 at 21,915,926 bp
  • A to G, chromosome 9 at 46,242,524 bp
  • A to G, chromosome 9 at 62,796,800 bp
  • A to T, chromosome 9 at 88,847,515 bp
  • C to A, chromosome 9 at 119,943,940 bp
  • T to A, chromosome 9 at 120,557,542 bp
  • A to G, chromosome 10 at 76,178,197 bp
  • G to A, chromosome 10 at 128,807,579 bp
  • C to G, chromosome 11 at 76,790,838 bp
  • C to A, chromosome 11 at 77,953,873 bp
  • A to T, chromosome 11 at 97,038,779 bp
  • G to A, chromosome 11 at 100,629,956 bp
  • G to T, chromosome 11 at 111,024,723 bp
  • C to A, chromosome 11 at 114,798,929 bp
  • G to A, chromosome 12 at 69,721,331 bp
  • A to G, chromosome 12 at 78,637,503 bp
  • A to T, chromosome 12 at 114,583,982 bp
  • C to T, chromosome 13 at 23,855,858 bp
  • T to A, chromosome 13 at 49,693,135 bp
  • A to G, chromosome 13 at 65,033,062 bp
  • A to G, chromosome 13 at 109,740,421 bp
  • T to A, chromosome 14 at 51,569,600 bp
  • T to C, chromosome 16 at 17,325,114 bp
  • C to T, chromosome 16 at 22,862,619 bp
  • T to C, chromosome 16 at 32,995,852 bp
  • T to C, chromosome 16 at 59,329,065 bp
  • G to A, chromosome 16 at 69,858,752 bp
  • T to A, chromosome 16 at 92,077,634 bp
  • A to T, chromosome 17 at 15,368,954 bp
  • A to G, chromosome 17 at 35,849,033 bp
  • T to A, chromosome 19 at 5,046,699 bp
  • T to A, chromosome 19 at 9,012,056 bp
  • T to C, chromosome 19 at 11,303,939 bp
  • A to G, chromosome 19 at 44,215,637 bp
  • T to A, chromosome 19 at 46,323,342 bp
  • C to A, chromosome 19 at 60,766,826 bp
  • A to G, chromosome 19 at 61,226,597 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0054 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038348-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.