Strain Name:
C57BL/6J-MtgxR0066Btlr/Mmmh
Stock Number:
038357-MU
Citation ID:
RRID:MMRRC_038357-MU
Other Names:
R0066 (G1), C57BL/6J-MtgxR0066Btlr
Major Collection:

Gene Information

Gene Symbol: Dst [MGI:104627] (Mus musculus (mouse))
Name: dystonin
Synonyms: A830042E19Rik, ah, 2310001O04Rik, Bpag, bullous pemphigoid antigen 1, athetoid, nmf203, nmf339, bullous pemphigoid antigen 1, Macf2, BPAG1-n, Bpag1, BPAG1
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Macf1 [MGI:108559] (Mus musculus (mouse))
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Usp7 [MGI:2182061] (Mus musculus (mouse))
Name: ubiquitin specific peptidase 7
Synonyms: 2210010O09Rik
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Plekha7 [MGI:2445094] (Mus musculus (mouse))
Name: pleckstrin homology domain containing, family A member 7
Synonyms: A430081P20Rik
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Sptan1 [MGI:98386] (Mus musculus (mouse))
Name: spectrin alpha, non-erythrocytic 1
Synonyms: 2610027H02Rik, alpha-fodrin, Spna2, alphaII-spectrin, Spna-2
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Plcb4 [MGI:107464] (Mus musculus (mouse))
Name: phospholipase C, beta 4
Synonyms: C230058B11Rik, A930039J07Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Aco2 [MGI:87880] (Mus musculus (mouse))
Name: aconitase 2, mitochondrial
Synonyms: Aco3, Aco-2, D10Wsu183e
Chromosome: 15
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 30,977,533 bp
  • C to A, chromosome 1 at 34,189,553 bp
  • A to G, chromosome 1 at 43,981,748 bp
  • A to G, chromosome 1 at 54,356,691 bp
  • A to T, chromosome 1 at 55,713,475 bp
  • A to C, chromosome 1 at 155,911,218 bp
  • A to G, chromosome 1 at 162,407,361 bp
  • T to C, chromosome 2 at 4,473,152 bp
  • T to C, chromosome 2 at 14,261,200 bp
  • C to A, chromosome 2 at 30,003,667 bp
  • C to T, chromosome 2 at 30,092,535 bp
  • T to C, chromosome 2 at 34,795,306 bp
  • T to A, chromosome 2 at 52,306,530 bp
  • T to G, chromosome 2 at 64,938,492 bp
  • T to C, chromosome 2 at 67,512,140 bp
  • A to G, chromosome 2 at 111,138,939 bp
  • T to C, chromosome 2 at 135,961,769 bp
  • A to T, chromosome 2 at 154,266,841 bp
  • T to A, chromosome 2 at 172,373,056 bp
  • T to C, chromosome 3 at 95,862,885 bp
  • A to G, chromosome 3 at 116,543,829 bp
  • G to T, chromosome 3 at 122,953,307 bp
  • G to A, chromosome 3 at 145,545,144 bp
  • G to A, chromosome 4 at 16,123,868 bp
  • G to T, chromosome 4 at 41,119,572 bp
  • A to G, chromosome 4 at 41,298,338 bp
  • G to T, chromosome 4 at 43,632,329 bp
  • A to G, chromosome 4 at 43,930,936 bp
  • G to A, chromosome 4 at 123,432,150 bp
  • A to G, chromosome 4 at 132,113,988 bp
  • G to A, chromosome 4 at 147,885,445 bp
  • T to C, chromosome 5 at 99,964,701 bp
  • T to A, chromosome 5 at 105,290,900 bp
  • T to A, chromosome 5 at 110,101,502 bp
  • T to C, chromosome 5 at 114,436,465 bp
  • T to A, chromosome 5 at 120,758,875 bp
  • T to G, chromosome 5 at 124,573,795 bp
  • A to T, chromosome 5 at 124,763,076 bp
  • A to T, chromosome 5 at 144,284,562 bp
  • A to T, chromosome 6 at 42,486,935 bp
  • T to A, chromosome 6 at 48,702,470 bp
  • A to T, chromosome 6 at 87,714,245 bp
  • A to T, chromosome 6 at 133,292,324 bp
  • A to T, chromosome 7 at 17,909,066 bp
  • C to T, chromosome 7 at 29,005,567 bp
  • G to A, chromosome 7 at 35,287,227 bp
  • T to C, chromosome 7 at 42,927,090 bp
  • C to T, chromosome 7 at 44,844,071 bp
  • T to C, chromosome 7 at 86,800,756 bp
  • A to G, chromosome 7 at 101,278,424 bp
  • A to T, chromosome 7 at 104,148,938 bp
  • T to C, chromosome 7 at 116,157,508 bp
  • C to T, chromosome 7 at 125,576,231 bp
  • A to T, chromosome 7 at 127,374,600 bp
  • A to T, chromosome 8 at 3,613,880 bp
  • T to A, chromosome 8 at 72,711,856 bp
  • C to T, chromosome 8 at 105,328,225 bp
  • A to G, chromosome 8 at 106,922,898 bp
  • G to T, chromosome 8 at 109,620,471 bp
  • G to A, chromosome 8 at 111,686,637 bp
  • A to G, chromosome 9 at 22,207,881 bp
  • A to C, chromosome 9 at 24,414,409 bp
  • G to A, chromosome 9 at 24,646,383 bp
  • T to C, chromosome 9 at 40,092,687 bp
  • C to A, chromosome 9 at 50,998,533 bp
  • A to T, chromosome 9 at 56,888,134 bp
  • T to A, chromosome 9 at 64,319,165 bp
  • A to T, chromosome 9 at 105,702,213 bp
  • A to G, chromosome 9 at 111,272,463 bp
  • C to A, chromosome 10 at 79,961,103 bp
  • T to C, chromosome 10 at 130,425,901 bp
  • T to A, chromosome 11 at 68,699,491 bp
  • A to T, chromosome 11 at 82,897,572 bp
  • T to C, chromosome 11 at 100,757,800 bp
  • A to T, chromosome 11 at 101,265,435 bp
  • A to G, chromosome 11 at 107,062,136 bp
  • T to G, chromosome 11 at 115,429,517 bp
  • T to A, chromosome 11 at 121,503,764 bp
  • T to C, chromosome 12 at 16,654,119 bp
  • A to T, chromosome 12 at 105,648,449 bp
  • A to G, chromosome 12 at 114,487,369 bp
  • A to C, chromosome 12 at 117,276,602 bp
  • A to G, chromosome 12 at 118,126,886 bp
  • A to T, chromosome 13 at 22,244,471 bp
  • A to T, chromosome 13 at 22,279,239 bp
  • T to A, chromosome 13 at 23,478,485 bp
  • G to A, chromosome 13 at 67,259,609 bp
  • C to T, chromosome 14 at 31,157,070 bp
  • T to A, chromosome 14 at 50,393,202 bp
  • A to T, chromosome 14 at 110,749,932 bp
  • T to A, chromosome 15 at 68,995,037 bp
  • T to C, chromosome 15 at 81,903,465 bp
  • T to A, chromosome 15 at 82,391,757 bp
  • T to A, chromosome 15 at 89,474,336 bp
  • A to T, chromosome 16 at 8,691,418 bp
  • C to A, chromosome 16 at 17,574,011 bp
  • A to G, chromosome 16 at 18,225,200 bp
  • T to A, chromosome 16 at 19,566,049 bp
  • A to G, chromosome 16 at 21,477,523 bp
  • T to C, chromosome 16 at 34,203,957 bp
  • G to A, chromosome 16 at 44,909,674 bp
  • A to T, chromosome 17 at 6,201,733 bp
  • T to C, chromosome 17 at 20,889,736 bp
  • A to G, chromosome 17 at 33,601,703 bp
  • A to G, chromosome 17 at 56,128,271 bp
  • A to T, chromosome 17 at 56,786,736 bp
  • T to C, chromosome 18 at 24,599,269 bp
  • A to G, chromosome 18 at 36,742,107 bp
  • A to T, chromosome 18 at 37,996,707 bp
  • T to C, chromosome 18 at 44,028,763 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R0066 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    038357-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.