Strain Name:
C57BL/6J-MtgxR0114Btlr/Mmmh
Stock Number:
038400-MU
Citation ID:
RRID:MMRRC_038400-MU
Other Names:
R0114 (G1), C57BL/6J-MtgxR0114Btlr
Major Collection:

Gene Information

Gene Symbol: Limch1 [MGI:1924819] (Mus musculus (mouse))
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Rad54l2 [MGI:1933196] (Mus musculus (mouse))
Name: RAD54 like 2 (S. cerevisiae)
Synonyms: Arip4, G630026H09Rik, Srisnf2l
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Utp23 [MGI:1925831] (Mus musculus (mouse))
Name: UTP23 small subunit processome component
Synonyms: D530033C11Rik, 1700010I21Rik
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Lgi3 [MGI:2182619] (Mus musculus (mouse))
Name: leucine-rich repeat LGI family, member 3
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Sema4b [MGI:107559] (Mus musculus (mouse))
Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Synonyms: Semac, SemC
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Tfr2 [MGI:1354956] (Mus musculus (mouse))
Name: transferrin receptor 2
Synonyms: Tfr2, Trfr2
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Usp28 [MGI:2442293] (Mus musculus (mouse))
Name: ubiquitin specific peptidase 28
Synonyms: 9830148O20Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 30,805,443 bp
  • G to T, chromosome 1 at 59,815,340 bp
  • T to C, chromosome 1 at 123,486,092 bp
  • T to C, chromosome 1 at 177,067,251 bp
  • A to G, chromosome 1 at 181,120,391 bp
  • G to C, chromosome 2 at 76,707,093 bp
  • A to G, chromosome 2 at 84,983,456 bp
  • A to G, chromosome 2 at 91,124,494 bp
  • A to T, chromosome 2 at 91,620,112 bp
  • G to A, chromosome 2 at 119,382,960 bp
  • A to G, chromosome 2 at 126,653,574 bp
  • A to T, chromosome 2 at 147,029,779 bp
  • A to G, chromosome 2 at 165,424,581 bp
  • G to T, chromosome 2 at 168,710,856 bp
  • T to C, chromosome 3 at 51,448,438 bp
  • T to C, chromosome 4 at 41,605,686 bp
  • G to T, chromosome 4 at 43,422,055 bp
  • T to A, chromosome 4 at 63,608,459 bp
  • A to T, chromosome 4 at 106,734,004 bp
  • C to A, chromosome 4 at 138,608,858 bp
  • T to C, chromosome 4 at 140,583,883 bp
  • T to C, chromosome 4 at 145,288,047 bp
  • C to T, chromosome 5 at 67,036,084 bp
  • T to A, chromosome 5 at 77,343,263 bp
  • T to C, chromosome 5 at 137,577,465 bp
  • A to T, chromosome 6 at 18,282,448 bp
  • T to C, chromosome 6 at 85,619,803 bp
  • A to G, chromosome 6 at 122,040,648 bp
  • A to G, chromosome 6 at 146,312,879 bp
  • A to G, chromosome 6 at 146,998,233 bp
  • G to A, chromosome 6 at 148,412,830 bp
  • A to G, chromosome 7 at 20,024,056 bp
  • T to C, chromosome 7 at 56,153,774 bp
  • A to G, chromosome 7 at 80,219,078 bp
  • A to G, chromosome 7 at 80,378,035 bp
  • G to A, chromosome 7 at 84,347,530 bp
  • A to T, chromosome 7 at 102,542,731 bp
  • A to G, chromosome 7 at 120,775,380 bp
  • A to C, chromosome 7 at 128,249,494 bp
  • A to T, chromosome 7 at 141,103,239 bp
  • A to G, chromosome 8 at 5,640,359 bp
  • A to T, chromosome 8 at 123,288,491 bp
  • T to G, chromosome 8 at 124,883,357 bp
  • A to T, chromosome 9 at 22,353,346 bp
  • C to A, chromosome 9 at 49,039,023 bp
  • T to G, chromosome 9 at 62,735,293 bp
  • T to C, chromosome 9 at 62,760,302 bp
  • T to A, chromosome 9 at 66,461,846 bp
  • T to C, chromosome 9 at 70,803,781 bp
  • T to A, chromosome 9 at 88,584,160 bp
  • T to C, chromosome 9 at 106,713,455 bp
  • C to A, chromosome 10 at 26,993,068 bp
  • T to C, chromosome 10 at 28,985,982 bp
  • C to A, chromosome 10 at 88,433,400 bp
  • T to C, chromosome 10 at 119,216,522 bp
  • T to A, chromosome 10 at 129,669,598 bp
  • T to A, chromosome 11 at 32,239,784 bp
  • G to A, chromosome 11 at 51,027,604 bp
  • G to A, chromosome 11 at 51,586,653 bp
  • C to T, chromosome 11 at 54,487,801 bp
  • A to T, chromosome 11 at 62,146,313 bp
  • T to C, chromosome 11 at 72,195,799 bp
  • A to T, chromosome 11 at 73,710,662 bp
  • G to A, chromosome 11 at 85,326,905 bp
  • G to T, chromosome 11 at 119,414,587 bp
  • T to C, chromosome 12 at 81,996,095 bp
  • T to A, chromosome 12 at 103,576,374 bp
  • A to T, chromosome 13 at 32,227,281 bp
  • G to A, chromosome 13 at 34,890,488 bp
  • A to G, chromosome 14 at 37,068,045 bp
  • C to T, chromosome 14 at 70,531,029 bp
  • T to C, chromosome 15 at 51,882,511 bp
  • C to T, chromosome 15 at 76,690,799 bp
  • T to C, chromosome 15 at 81,337,959 bp
  • T to A, chromosome 15 at 103,455,028 bp
  • A to T, chromosome 16 at 29,629,635 bp
  • C to A, chromosome 16 at 85,799,614 bp
  • G to A, chromosome 17 at 23,670,239 bp
  • A to T, chromosome 17 at 37,589,415 bp
  • A to G, chromosome 18 at 47,290,177 bp
  • A to T, chromosome 18 at 74,742,171 bp
  • C to A, chromosome 19 at 6,228,431 bp
  • T to C, chromosome 19 at 37,686,633 bp
  • C to T, chromosome 19 at 46,366,504 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R0114 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    038400-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.