Strain Name:
C57BL/6J-MtgxR0126Btlr/Mmmh
Stock Number:
038411-MU
Citation ID:
RRID:MMRRC_038411-MU
Other Names:
R0126 (G1), C57BL/6J-MtgxR0126Btlr
Major Collection:

Gene Information

Gene Symbol: Casq2 [MGI:1309469] (Mus musculus (mouse))
Name: calsequestrin 2
Synonyms: cardiac calsequestrin, ESTM52, cCSQ, Csq2
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Utrn [MGI:104631] (Mus musculus (mouse))
Name: utrophin
Synonyms: DRP, G-utrophin, Dmdl
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Tlr9 [MGI:1932389] (Mus musculus (mouse))
Name: toll-like receptor 9
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Prdm16 [MGI:1917923] (Mus musculus (mouse))
Name: PR domain containing 16
Synonyms: csp1, line 27, Mel1, 5730557K01Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Iqgap1 [MGI:1352757] (Mus musculus (mouse))
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Rpa1 [MGI:1915525] (Mus musculus (mouse))
Name: replication protein A1
Synonyms: RF-A, RP-A, Rpa, 5031405K23Rik, 70kDa
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Jmjd1c [MGI:1918614] (Mus musculus (mouse))
Name: jumonji domain containing 1C
Synonyms: D630035I23Rik, 5430433L24Rik, TRIP8
Chromosome: 10
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 75,312,538 bp
  • G to A, chromosome 1 at 87,411,875 bp
  • C to T, chromosome 1 at 171,276,165 bp
  • T to C, chromosome 1 at 182,279,692 bp
  • T to C, chromosome 2 at 25,443,730 bp
  • T to A, chromosome 2 at 86,754,637 bp
  • C to T, chromosome 2 at 118,690,332 bp
  • T to A, chromosome 2 at 118,790,338 bp
  • T to C, chromosome 2 at 121,906,084 bp
  • T to C, chromosome 2 at 122,798,027 bp
  • T to C, chromosome 2 at 152,612,579 bp
  • A to G, chromosome 3 at 82,037,911 bp
  • A to G, chromosome 3 at 102,133,399 bp
  • A to T, chromosome 3 at 123,079,862 bp
  • T to C, chromosome 4 at 45,912,866 bp
  • T to A, chromosome 4 at 116,256,268 bp
  • C to T, chromosome 4 at 145,898,943 bp
  • A to T, chromosome 4 at 154,328,838 bp
  • T to A, chromosome 4 at 156,203,647 bp
  • T to C, chromosome 5 at 34,613,579 bp
  • T to C, chromosome 5 at 64,822,103 bp
  • G to T, chromosome 5 at 73,607,088 bp
  • C to T, chromosome 5 at 87,980,195 bp
  • C to G, chromosome 5 at 110,396,040 bp
  • T to A, chromosome 5 at 134,902,788 bp
  • C to A, chromosome 5 at 135,132,323 bp
  • A to T, chromosome 5 at 144,805,750 bp
  • G to T, chromosome 6 at 89,976,953 bp
  • C to T, chromosome 6 at 118,165,995 bp
  • A to T, chromosome 7 at 24,260,724 bp
  • T to A, chromosome 7 at 28,351,083 bp
  • A to G, chromosome 7 at 80,738,322 bp
  • T to A, chromosome 7 at 90,396,589 bp
  • A to G, chromosome 7 at 102,877,140 bp
  • A to G, chromosome 7 at 126,863,442 bp
  • A to G, chromosome 7 at 144,031,355 bp
  • T to C, chromosome 8 at 24,970,737 bp
  • C to T, chromosome 8 at 72,058,400 bp
  • A to T, chromosome 8 at 94,554,933 bp
  • A to C, chromosome 8 at 104,140,682 bp
  • T to C, chromosome 8 at 119,762,350 bp
  • A to G, chromosome 8 at 124,683,291 bp
  • A to T, chromosome 9 at 21,975,812 bp
  • A to G, chromosome 9 at 38,737,807 bp
  • A to T, chromosome 9 at 75,269,525 bp
  • T to A, chromosome 9 at 87,729,653 bp
  • T to A, chromosome 9 at 106,225,682 bp
  • A to G, chromosome 9 at 108,969,583 bp
  • T to A, chromosome 10 at 12,711,475 bp
  • T to A, chromosome 10 at 23,920,547 bp
  • T to C, chromosome 10 at 67,219,326 bp
  • T to C, chromosome 10 at 78,278,056 bp
  • A to T, chromosome 10 at 80,068,853 bp
  • T to C, chromosome 10 at 88,676,062 bp
  • T to A, chromosome 10 at 88,952,292 bp
  • T to C, chromosome 11 at 40,705,954 bp
  • A to T, chromosome 11 at 44,410,218 bp
  • A to G, chromosome 11 at 59,437,987 bp
  • C to A, chromosome 11 at 61,224,558 bp
  • A to C, chromosome 11 at 69,167,471 bp
  • T to G, chromosome 11 at 69,747,425 bp
  • A to T, chromosome 11 at 70,641,033 bp
  • A to C, chromosome 11 at 75,318,529 bp
  • G to C, chromosome 11 at 116,124,604 bp
  • T to A, chromosome 11 at 116,766,584 bp
  • A to G, chromosome 12 at 29,851,720 bp
  • T to C, chromosome 12 at 30,201,261 bp
  • T to C, chromosome 13 at 17,695,752 bp
  • T to C, chromosome 13 at 41,032,596 bp
  • A to T, chromosome 13 at 99,228,621 bp
  • G to T, chromosome 13 at 112,729,257 bp
  • C to T, chromosome 14 at 63,497,593 bp
  • A to T, chromosome 15 at 21,583,945 bp
  • A to G, chromosome 15 at 28,246,319 bp
  • A to G, chromosome 15 at 31,462,005 bp
  • G to A, chromosome 15 at 76,243,066 bp
  • A to T, chromosome 15 at 76,669,254 bp
  • A to T, chromosome 15 at 83,621,063 bp
  • C to A, chromosome 15 at 102,358,460 bp
  • A to T, chromosome 16 at 4,084,063 bp
  • A to T, chromosome 16 at 55,866,229 bp
  • A to T, chromosome 16 at 87,425,640 bp
  • G to A, chromosome 17 at 85,083,242 bp
  • A to G, chromosome 18 at 20,340,878 bp
  • T to C, chromosome 19 at 5,112,746 bp
  • T to G, chromosome 19 at 55,241,166 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0126 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038411-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.