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Strain Name:
C57BL/6J-MtgxR0193Btlr/Mmmh
Stock Number:
038452-MU
Citation ID:
RRID:MMRRC_038452-MU
Other Names:
R0193 (G1), C57BL/6J-MtgxR0193Btlr
Major Collection:

Strain Information

Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953
Erbb4
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: ErbB4, Her4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13869
HGNC: HGNC:3432
Homologene: 21084
Trdmt1
Name: tRNA aspartic acid methyltransferase 1
Synonyms: Rnmt2, Dnmt2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13434
HGNC: HGNC:2977
Homologene: 3249
Mix23
Name: mitochondrial matrix import factor 23
Synonyms: A930007B11Rik, Ccdc58
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 381045
VEGA: 16
Homologene: 18028
Mtss1
Name: MTSS I-BAR domain containing 1
Synonyms: MIM, D130001D01Rik, 2310003N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 211401
VEGA: 15
Homologene: 8841
Pigl
Name: phosphatidylinositol glycan anchor biosynthesis, class L
Synonyms: LOC327942
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327942
HGNC: HGNC:8966
Homologene: 3159
Mkks
Name: McKusick-Kaufman syndrome
Synonyms: 1300013E18Rik, Bbs6
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 59030
HGNC: HGNC:7108
Homologene: 10318
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,358,917 bp
  • T to C, chromosome 1 at 68,043,960 bp
  • A to G, chromosome 1 at 87,509,334 bp
  • A to T, chromosome 1 at 136,468,438 bp
  • A to G, chromosome 1 at 161,085,988 bp
  • A to G, chromosome 1 at 162,649,035 bp
  • A to T, chromosome 1 at 171,025,760 bp
  • C to A, chromosome 2 at 13,544,617 bp
  • C to T, chromosome 2 at 29,179,673 bp
  • C to T, chromosome 2 at 30,187,186 bp
  • T to A, chromosome 2 at 88,930,283 bp
  • A to C, chromosome 2 at 122,052,027 bp
  • T to C, chromosome 2 at 136,877,606 bp
  • A to G, chromosome 3 at 64,491,530 bp
  • T to C, chromosome 3 at 64,691,039 bp
  • A to T, chromosome 3 at 88,287,088 bp
  • A to G, chromosome 3 at 90,481,339 bp
  • T to C, chromosome 3 at 129,988,984 bp
  • A to T, chromosome 3 at 136,066,518 bp
  • G to A, chromosome 4 at 45,914,803 bp
  • G to T, chromosome 4 at 138,146,376 bp
  • T to C, chromosome 4 at 141,412,316 bp
  • G to A, chromosome 4 at 149,634,796 bp
  • C to T, chromosome 4 at 150,930,717 bp
  • C to T, chromosome 4 at 155,426,115 bp
  • T to C, chromosome 4 at 155,655,673 bp
  • A to T, chromosome 5 at 9,422,359 bp
  • T to C, chromosome 5 at 43,736,118 bp
  • T to C, chromosome 5 at 67,027,539 bp
  • C to T, chromosome 5 at 77,320,076 bp
  • T to C, chromosome 5 at 86,032,403 bp
  • A to T, chromosome 5 at 128,788,356 bp
  • T to C, chromosome 5 at 139,751,730 bp
  • T to A, chromosome 6 at 13,583,313 bp
  • T to C, chromosome 6 at 23,599,440 bp
  • A to G, chromosome 6 at 64,063,953 bp
  • T to A, chromosome 6 at 67,272,884 bp
  • A to G, chromosome 6 at 72,436,195 bp
  • C to T, chromosome 6 at 113,419,237 bp
  • C to T, chromosome 6 at 118,602,402 bp
  • G to T, chromosome 6 at 121,410,724 bp
  • A to G, chromosome 7 at 12,668,027 bp
  • T to A, chromosome 7 at 25,287,140 bp
  • C to A, chromosome 7 at 25,287,141 bp
  • T to A, chromosome 7 at 46,377,112 bp
  • C to T, chromosome 7 at 80,261,930 bp
  • G to T, chromosome 7 at 103,346,204 bp
  • C to T, chromosome 7 at 105,764,983 bp
  • A to G, chromosome 8 at 13,570,233 bp
  • T to G, chromosome 8 at 27,031,764 bp
  • T to C, chromosome 9 at 44,507,406 bp
  • T to C, chromosome 9 at 121,767,666 bp
  • T to C, chromosome 10 at 79,381,295 bp
  • G to T, chromosome 11 at 50,389,852 bp
  • A to G, chromosome 11 at 62,503,748 bp
  • A to T, chromosome 11 at 62,715,029 bp
  • A to G, chromosome 11 at 69,048,544 bp
  • A to G, chromosome 11 at 72,070,492 bp
  • G to T, chromosome 11 at 72,972,220 bp
  • C to T, chromosome 11 at 80,353,574 bp
  • A to G, chromosome 11 at 83,121,843 bp
  • T to A, chromosome 11 at 101,048,482 bp
  • A to G, chromosome 11 at 102,352,684 bp
  • A to T, chromosome 11 at 106,320,538 bp
  • A to G, chromosome 11 at 106,376,896 bp
  • A to G, chromosome 11 at 114,893,376 bp
  • A to T, chromosome 11 at 119,359,314 bp
  • A to G, chromosome 13 at 4,511,293 bp
  • G to A, chromosome 13 at 46,709,654 bp
  • C to T, chromosome 13 at 112,988,884 bp
  • A to G, chromosome 14 at 120,379,237 bp
  • G to A, chromosome 14 at 123,846,546 bp
  • G to T, chromosome 15 at 36,161,883 bp
  • T to C, chromosome 15 at 58,944,017 bp
  • T to C, chromosome 15 at 74,572,156 bp
  • T to C, chromosome 15 at 101,479,363 bp
  • G to A, chromosome 16 at 4,627,504 bp
  • A to G, chromosome 16 at 34,187,514 bp
  • T to C, chromosome 16 at 36,082,814 bp
  • A to G, chromosome 16 at 44,449,210 bp
  • A to T, chromosome 16 at 56,265,049 bp
  • T to C, chromosome 16 at 64,785,688 bp
  • T to A, chromosome 16 at 64,926,749 bp
  • G to T, chromosome 17 at 24,510,551 bp
  • T to C, chromosome 17 at 25,990,999 bp
  • A to G, chromosome 17 at 29,140,307 bp
  • C to T, chromosome 17 at 36,671,332 bp
  • C to T, chromosome 17 at 56,393,952 bp
  • G to A, chromosome 17 at 66,500,362 bp
  • C to T, chromosome 18 at 64,561,636 bp
  • T to G, chromosome 19 at 5,052,652 bp
  • T to A, chromosome 19 at 5,695,846 bp
  • T to A, chromosome 19 at 8,847,429 bp
  • T to C, chromosome X at 20,862,496 bp
  • T to A, chromosome X at 143,100,278 bp
  • T to C, chromosome X at 152,223,403 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0193 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038452-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.