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Strain Name:
C57BL/6J-MtgxR0195Btlr/Mmmh
Stock Number:
038454-MU
Citation ID:
RRID:MMRRC_038454-MU
Other Names:
R0195 (G1), C57BL/6J-MtgxR0195Btlr
Major Collection:

Strain Information

Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22359
Homologene: 443
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Orc1
Name: origin recognition complex, subunit 1
Synonyms: MmORC1, Orc1l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18392
HGNC: HGNC:8487
Homologene: 31221
Zmym1
Name: zinc finger, MYM domain containing 1
Synonyms: 5830412B09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68310
Homologene: 32904
Srsf11
Name: serine and arginine-rich splicing factor 11
Synonyms: 0610009J05Rik, 2610019N13Rik, Sfrs11
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69207
Homologene: 36164
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 139,479,135 bp
  • G to T, chromosome 2 at 74,659,044 bp
  • T to C, chromosome 2 at 144,548,106 bp
  • T to A, chromosome 2 at 166,940,088 bp
  • T to C, chromosome 3 at 32,992,953 bp
  • T to A, chromosome 3 at 55,054,364 bp
  • T to A, chromosome 3 at 57,293,059 bp
  • A to G, chromosome 3 at 94,265,922 bp
  • T to G, chromosome 3 at 98,316,550 bp
  • A to T, chromosome 3 at 122,276,161 bp
  • A to G, chromosome 3 at 158,036,535 bp
  • T to C, chromosome 4 at 18,906,988 bp
  • A to T, chromosome 4 at 19,280,975 bp
  • G to A, chromosome 4 at 52,910,849 bp
  • A to T, chromosome 4 at 58,089,514 bp
  • C to T, chromosome 4 at 108,614,308 bp
  • A to G, chromosome 4 at 123,434,916 bp
  • A to G, chromosome 4 at 126,463,691 bp
  • A to G, chromosome 4 at 127,047,911 bp
  • A to G, chromosome 5 at 24,756,038 bp
  • A to G, chromosome 5 at 34,610,646 bp
  • A to T, chromosome 5 at 43,770,798 bp
  • T to C, chromosome 5 at 77,006,494 bp
  • G to A, chromosome 5 at 120,756,145 bp
  • A to G, chromosome 6 at 28,243,847 bp
  • T to A, chromosome 6 at 48,486,636 bp
  • A to G, chromosome 6 at 113,466,137 bp
  • A to T, chromosome 6 at 113,793,874 bp
  • A to C, chromosome 6 at 122,851,155 bp
  • A to T, chromosome 6 at 128,487,478 bp
  • G to T, chromosome 7 at 23,835,585 bp
  • A to C, chromosome 7 at 100,546,353 bp
  • A to T, chromosome 7 at 100,938,697 bp
  • A to G, chromosome 7 at 104,382,429 bp
  • A to T, chromosome 7 at 120,077,775 bp
  • A to C, chromosome 7 at 142,296,697 bp
  • A to T, chromosome 8 at 10,315,538 bp
  • T to A, chromosome 8 at 25,680,693 bp
  • T to A, chromosome 8 at 40,681,766 bp
  • G to T, chromosome 8 at 43,520,270 bp
  • A to G, chromosome 9 at 21,142,280 bp
  • A to G, chromosome 10 at 19,005,713 bp
  • A to G, chromosome 10 at 60,317,059 bp
  • C to T, chromosome 11 at 65,895,905 bp
  • T to A, chromosome 11 at 67,709,708 bp
  • T to A, chromosome 11 at 77,501,073 bp
  • A to G, chromosome 11 at 82,810,163 bp
  • T to A, chromosome 11 at 82,937,828 bp
  • T to A, chromosome 11 at 98,821,493 bp
  • C to G, chromosome 11 at 100,512,974 bp
  • C to T, chromosome 11 at 105,385,525 bp
  • T to G, chromosome 11 at 115,564,531 bp
  • A to T, chromosome 12 at 44,584,845 bp
  • C to T, chromosome 12 at 73,364,321 bp
  • C to T, chromosome 12 at 103,418,961 bp
  • T to C, chromosome 12 at 103,985,872 bp
  • T to A, chromosome 13 at 42,156,153 bp
  • G to T, chromosome 13 at 51,171,447 bp
  • A to T, chromosome 14 at 19,161,362 bp
  • T to C, chromosome 14 at 31,365,581 bp
  • T to G, chromosome 14 at 68,527,627 bp
  • G to A, chromosome 15 at 4,763,471 bp
  • A to G, chromosome 15 at 35,471,899 bp
  • A to G, chromosome 15 at 58,099,954 bp
  • G to A, chromosome 15 at 101,813,191 bp
  • G to A, chromosome 15 at 102,222,183 bp
  • T to A, chromosome 16 at 17,899,575 bp
  • T to A, chromosome 16 at 79,034,334 bp
  • A to T, chromosome 17 at 20,574,055 bp
  • A to C, chromosome 17 at 22,992,806 bp
  • A to G, chromosome 17 at 27,114,114 bp
  • T to C, chromosome 17 at 40,207,731 bp
  • A to G, chromosome 17 at 57,081,499 bp
  • C to T, chromosome 17 at 66,049,948 bp
  • A to G, chromosome 17 at 80,381,434 bp
  • A to G, chromosome 18 at 58,969,870 bp
  • A to T, chromosome 19 at 27,238,386 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0195 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038454-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.