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Strain Name:
C57BL/6J-MtgxR0230Btlr/Mmmh
Stock Number:
038473-MU
Citation ID:
RRID:MMRRC_038473-MU
Other Names:
R0230 (G1), C57BL/6J-MtgxR0230Btlr
Major Collection:

Strain Information

Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Slco1a5
Name: solute carrier organic anion transporter family, member 1a5
Synonyms: Oatp3, Slc21a7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108096
Homologene: 56603
Lefty1
Name: left right determination factor 1
Synonyms: lefty-1, Stra3, Lefty, Ebaf
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13590
HGNC: HGNC:6552
Homologene: 49231
Pex7
Name: peroxisomal biogenesis factor 7
Synonyms: peroxisome biogenesis factor 7
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18634
HGNC: HGNC:8860
Homologene: 242
Map2k6
Name: mitogen-activated protein kinase kinase 6
Synonyms: MAP kinase kinase 6, MKK6, SAPKK3, Prkmk6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26399
HGNC: HGNC:6846
Homologene: 55686
Sptan1
Name: spectrin alpha, non-erythrocytic 1
Synonyms: alpha-fodrin, Spna-2, 2610027H02Rik, Spna2, alphaII-spectrin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20740
Homologene: 2353
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 46,219,348 bp
  • C to T, chromosome 1 at 139,446,935 bp
  • A to G, chromosome 1 at 140,246,345 bp
  • C to T, chromosome 1 at 161,062,787 bp
  • A to G, chromosome 1 at 179,423,428 bp
  • T to A, chromosome 1 at 180,937,014 bp
  • C to T, chromosome 1 at 188,850,104 bp
  • T to A, chromosome 2 at 30,010,692 bp
  • T to C, chromosome 2 at 30,194,075 bp
  • G to A, chromosome 2 at 36,688,616 bp
  • T to C, chromosome 2 at 66,726,284 bp
  • T to C, chromosome 2 at 76,737,434 bp
  • T to C, chromosome 2 at 86,171,542 bp
  • G to A, chromosome 2 at 127,435,845 bp
  • C to T, chromosome 2 at 154,317,075 bp
  • C to A, chromosome 2 at 155,578,406 bp
  • T to C, chromosome 2 at 163,559,085 bp
  • T to G, chromosome 2 at 168,912,230 bp
  • A to G, chromosome 3 at 53,478,962 bp
  • T to C, chromosome 3 at 69,575,340 bp
  • T to C, chromosome 3 at 79,644,975 bp
  • T to C, chromosome 3 at 100,106,827 bp
  • A to G, chromosome 3 at 105,918,737 bp
  • T to C, chromosome 3 at 113,558,430 bp
  • C to A, chromosome 4 at 59,189,739 bp
  • T to C, chromosome 4 at 102,597,510 bp
  • G to A, chromosome 5 at 88,489,655 bp
  • A to C, chromosome 5 at 89,156,336 bp
  • A to G, chromosome 5 at 103,527,131 bp
  • T to A, chromosome 5 at 148,918,650 bp
  • A to G, chromosome 6 at 3,463,336 bp
  • A to G, chromosome 6 at 121,324,303 bp
  • T to A, chromosome 6 at 142,236,328 bp
  • A to G, chromosome 6 at 144,209,338 bp
  • A to C, chromosome 6 at 145,819,974 bp
  • G to A, chromosome 7 at 24,612,579 bp
  • T to A, chromosome 7 at 28,156,825 bp
  • C to T, chromosome 7 at 109,824,764 bp
  • A to G, chromosome 7 at 121,746,761 bp
  • C to T, chromosome 8 at 72,058,400 bp
  • C to G, chromosome 8 at 111,315,062 bp
  • G to A, chromosome 9 at 21,700,872 bp
  • C to T, chromosome 9 at 50,695,507 bp
  • A to C, chromosome 9 at 106,072,366 bp
  • T to C, chromosome 9 at 110,420,001 bp
  • A to G, chromosome 9 at 113,685,293 bp
  • A to G, chromosome 10 at 19,904,585 bp
  • G to A, chromosome 10 at 80,055,004 bp
  • A to G, chromosome 10 at 94,799,347 bp
  • A to G, chromosome 11 at 44,996,122 bp
  • T to A, chromosome 11 at 61,159,197 bp
  • C to T, chromosome 11 at 65,855,315 bp
  • T to C, chromosome 11 at 72,306,555 bp
  • T to C, chromosome 11 at 80,477,750 bp
  • T to A, chromosome 11 at 84,893,333 bp
  • T to G, chromosome 11 at 98,203,991 bp
  • C to T, chromosome 11 at 110,496,455 bp
  • A to G, chromosome 12 at 84,425,204 bp
  • C to A, chromosome 12 at 117,983,056 bp
  • A to T, chromosome 13 at 38,197,705 bp
  • T to C, chromosome 13 at 67,294,116 bp
  • T to A, chromosome 13 at 77,112,748 bp
  • T to A, chromosome 13 at 96,491,086 bp
  • A to G, chromosome 14 at 18,280,185 bp
  • A to T, chromosome 14 at 54,973,933 bp
  • T to G, chromosome 15 at 71,446,037 bp
  • T to A, chromosome 15 at 82,182,085 bp
  • T to C, chromosome 16 at 20,733,275 bp
  • A to T, chromosome 16 at 21,220,775 bp
  • G to A, chromosome 16 at 23,114,099 bp
  • C to A, chromosome 16 at 36,937,854 bp
  • A to T, chromosome 16 at 44,365,576 bp
  • A to G, chromosome 16 at 57,046,996 bp
  • A to G, chromosome 17 at 24,701,333 bp
  • A to G, chromosome 17 at 38,175,950 bp
  • A to T, chromosome 17 at 43,732,012 bp
  • T to C, chromosome 17 at 70,929,133 bp
  • T to C, chromosome 18 at 6,440,168 bp
  • T to A, chromosome 18 at 22,452,326 bp
  • T to C, chromosome 18 at 48,047,739 bp
  • T to A, chromosome 19 at 9,118,519 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0230 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038473-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.