Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR0270Btlr/Mmmh
Stock Number:
038496-MU
Citation ID:
RRID:MMRRC_038496-MU
Other Names:
R0270 (G1), C57BL/6J-MtgxR0270Btlr
Major Collection:

Strain Information

Sphk2
Name: sphingosine kinase 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56632
Homologene: 32456
Acadm
Name: acyl-Coenzyme A dehydrogenase, medium chain
Synonyms: MCAD
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11364
HGNC: HGNC:89
Homologene: 3
Upf1
Name: UPF1 RNA helicase and ATPase
Synonyms: PNORF-1, B430202H16Rik, Rent1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19704
HGNC: HGNC:9962
Homologene: 2185
Gon4l
Name: gon-4 like
Synonyms: 1500041I23Rik, 2610100B20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76022
Homologene: 13002
Mbtps1
Name: membrane-bound transcription factor peptidase, site 1
Synonyms: subtilisin/kexin isozyme-1, SKI-1, S1P, 0610038M03Rik, site-1 protease
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56453
Homologene: 2808
Mov10
Name: Mov10 RISC complex RNA helicase
Synonyms: Mov-10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17454
HGNC: HGNC:7200
Homologene: 10365
Macroh2a1
Name: macroH2A.1 histone
Synonyms: mH2a1, MACROH2A1.2, H2AF12M, H2afy
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 26914
HGNC: HGNC:4740
Homologene: 3598
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 59,310,806 bp
  • T to C, chromosome 1 at 93,054,442 bp
  • T to A, chromosome 1 at 120,166,176 bp
  • T to A, chromosome 1 at 132,521,724 bp
  • T to C, chromosome 1 at 189,650,714 bp
  • A to T, chromosome 2 at 10,251,264 bp
  • C to T, chromosome 2 at 32,034,814 bp
  • A to T, chromosome 2 at 38,739,020 bp
  • A to T, chromosome 2 at 44,752,174 bp
  • C to A, chromosome 2 at 76,944,796 bp
  • A to C, chromosome 2 at 84,690,115 bp
  • T to A, chromosome 2 at 104,788,961 bp
  • C to A, chromosome 2 at 120,041,592 bp
  • T to C, chromosome 2 at 127,232,982 bp
  • T to A, chromosome 2 at 130,690,932 bp
  • T to C, chromosome 2 at 132,870,719 bp
  • A to T, chromosome 2 at 157,557,977 bp
  • T to A, chromosome 2 at 167,451,296 bp
  • A to G, chromosome 2 at 180,039,400 bp
  • A to G, chromosome 3 at 15,410,527 bp
  • C to A, chromosome 3 at 54,384,550 bp
  • T to A, chromosome 3 at 59,877,019 bp
  • T to G, chromosome 3 at 69,317,976 bp
  • G to A, chromosome 3 at 88,858,400 bp
  • A to G, chromosome 3 at 93,826,924 bp
  • C to A, chromosome 3 at 104,795,405 bp
  • C to T, chromosome 3 at 153,936,324 bp
  • A to T, chromosome 4 at 103,190,838 bp
  • C to T, chromosome 4 at 119,281,591 bp
  • T to A, chromosome 4 at 126,573,236 bp
  • T to G, chromosome 4 at 139,479,435 bp
  • G to A, chromosome 5 at 3,890,990 bp
  • C to T, chromosome 5 at 24,417,702 bp
  • C to A, chromosome 5 at 120,674,729 bp
  • A to T, chromosome 5 at 137,312,841 bp
  • T to G, chromosome 5 at 142,084,566 bp
  • T to A, chromosome 5 at 143,080,241 bp
  • A to G, chromosome 5 at 145,244,457 bp
  • T to A, chromosome 6 at 50,847,059 bp
  • C to T, chromosome 6 at 113,329,256 bp
  • A to T, chromosome 6 at 129,325,608 bp
  • A to G, chromosome 6 at 136,926,734 bp
  • T to A, chromosome 6 at 141,972,411 bp
  • C to T, chromosome 7 at 34,150,856 bp
  • T to A, chromosome 7 at 45,319,253 bp
  • T to C, chromosome 7 at 45,710,725 bp
  • T to C, chromosome 7 at 64,348,871 bp
  • G to A, chromosome 7 at 82,556,824 bp
  • A to G, chromosome 7 at 87,003,386 bp
  • T to C, chromosome 7 at 90,403,020 bp
  • T to G, chromosome 7 at 126,863,191 bp
  • T to A, chromosome 8 at 20,944,600 bp
  • C to T, chromosome 8 at 22,210,456 bp
  • T to A, chromosome 8 at 23,088,925 bp
  • C to T, chromosome 8 at 54,693,096 bp
  • G to A, chromosome 8 at 70,335,645 bp
  • T to A, chromosome 8 at 71,398,406 bp
  • A to T, chromosome 8 at 71,914,615 bp
  • A to T, chromosome 8 at 105,945,389 bp
  • G to A, chromosome 8 at 119,538,117 bp
  • C to T, chromosome 9 at 18,467,956 bp
  • T to A, chromosome 9 at 18,856,831 bp
  • A to T, chromosome 9 at 65,858,358 bp
  • A to G, chromosome 9 at 86,596,204 bp
  • G to T, chromosome 9 at 92,584,521 bp
  • A to G, chromosome 9 at 121,767,575 bp
  • A to G, chromosome 9 at 121,784,720 bp
  • A to G, chromosome 10 at 57,502,639 bp
  • C to T, chromosome 10 at 70,544,513 bp
  • C to A, chromosome 10 at 75,780,915 bp
  • T to A, chromosome 10 at 84,718,475 bp
  • T to C, chromosome 11 at 53,668,025 bp
  • T to A, chromosome 11 at 69,311,341 bp
  • T to G, chromosome 11 at 75,505,249 bp
  • A to T, chromosome 11 at 98,098,503 bp
  • A to T, chromosome 12 at 4,838,229 bp
  • C to T, chromosome 12 at 33,342,151 bp
  • A to G, chromosome 12 at 71,072,632 bp
  • A to G, chromosome 12 at 83,133,689 bp
  • A to T, chromosome 12 at 85,708,672 bp
  • G to A, chromosome 12 at 118,041,013 bp
  • T to C, chromosome 13 at 55,674,104 bp
  • G to A, chromosome 13 at 56,096,114 bp
  • A to T, chromosome 13 at 67,293,927 bp
  • A to T, chromosome 13 at 74,332,247 bp
  • T to A, chromosome 13 at 94,404,118 bp
  • A to G, chromosome 13 at 105,252,266 bp
  • T to A, chromosome 14 at 47,714,662 bp
  • T to C, chromosome 14 at 56,949,684 bp
  • T to A, chromosome 14 at 96,518,344 bp
  • T to C, chromosome 14 at 121,575,999 bp
  • G to A, chromosome 15 at 98,642,559 bp
  • T to A, chromosome 16 at 20,560,168 bp
  • A to G, chromosome 16 at 38,564,313 bp
  • G to A, chromosome 16 at 56,269,015 bp
  • A to G, chromosome 16 at 59,283,753 bp
  • G to T, chromosome 16 at 96,422,930 bp
  • A to C, chromosome 17 at 20,776,596 bp
  • T to A, chromosome 17 at 21,561,302 bp
  • G to A, chromosome 17 at 34,860,386 bp
  • T to C, chromosome 17 at 40,930,050 bp
  • T to C, chromosome 17 at 43,624,308 bp
  • T to A, chromosome 17 at 73,240,027 bp
  • C to A, chromosome 18 at 40,206,407 bp
  • T to C, chromosome 18 at 42,104,081 bp
  • A to G, chromosome 19 at 13,165,887 bp
  • A to G, chromosome 19 at 13,633,684 bp
  • A to T, chromosome 19 at 46,311,626 bp
  • A to G, chromosome 19 at 47,797,203 bp
  • T to A, chromosome 19 at 56,551,870 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0270 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038496-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.