Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR0271Btlr/Mmmh
Stock Number:
038497-MU
Citation ID:
RRID:MMRRC_038497-MU
Other Names:
R0271 (G1), C57BL/6J-MtgxR0271Btlr
Major Collection:

Strain Information

Nes
Name: nestin
Synonyms: RC2, Marc2, ESTM46, Ifaprc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Slc35a1
Name: solute carrier family 35 (CMP-sialic acid transporter), member 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 24060
Homologene: 38181
Khdc4
Name: KH domain containing 4, pre-mRNA splicing factor
Synonyms: 2810403A07Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74200
Homologene: 41761
Dip2c
Name: disco interacting protein 2 homolog C
Synonyms: 2900024P20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 208440
Homologene: 40996
Wdr43
Name: WD repeat domain 43
Synonyms: 2610318G08Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72515
VEGA: 17
Homologene: 38810
Ankrd17
Name: ankyrin repeat domain 17
Synonyms: Gtar, 4933425K22Rik, A130069E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81702
Homologene: 82403
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 54,428,812 bp
  • A to T, chromosome 2 at 30,269,822 bp
  • A to C, chromosome 2 at 77,920,858 bp
  • A to T, chromosome 2 at 85,904,289 bp
  • T to A, chromosome 2 at 89,418,158 bp
  • T to A, chromosome 2 at 112,243,245 bp
  • A to G, chromosome 2 at 152,867,367 bp
  • A to T, chromosome 2 at 181,995,954 bp
  • A to G, chromosome 3 at 75,246,578 bp
  • G to A, chromosome 3 at 87,978,642 bp
  • T to C, chromosome 3 at 88,686,329 bp
  • C to T, chromosome 3 at 108,086,716 bp
  • T to A, chromosome 4 at 34,664,125 bp
  • G to A, chromosome 4 at 58,952,391 bp
  • A to G, chromosome 4 at 106,449,049 bp
  • C to A, chromosome 4 at 107,066,461 bp
  • G to T, chromosome 4 at 141,250,607 bp
  • A to G, chromosome 4 at 154,926,597 bp
  • T to C, chromosome 5 at 90,254,799 bp
  • T to C, chromosome 5 at 112,809,685 bp
  • A to T, chromosome 6 at 128,519,514 bp
  • A to T, chromosome 7 at 24,137,131 bp
  • T to C, chromosome 7 at 90,403,020 bp
  • T to A, chromosome 7 at 103,979,630 bp
  • C to T, chromosome 8 at 54,693,096 bp
  • A to G, chromosome 8 at 95,845,878 bp
  • A to G, chromosome 8 at 99,111,715 bp
  • T to C, chromosome 8 at 123,272,441 bp
  • A to G, chromosome 9 at 26,920,666 bp
  • A to G, chromosome 9 at 45,333,688 bp
  • T to C, chromosome 9 at 80,386,879 bp
  • G to A, chromosome 10 at 7,919,158 bp
  • G to A, chromosome 10 at 58,575,823 bp
  • C to T, chromosome 10 at 94,837,918 bp
  • A to G, chromosome 11 at 29,848,949 bp
  • T to G, chromosome 11 at 59,056,742 bp
  • A to G, chromosome 11 at 70,616,648 bp
  • T to C, chromosome 11 at 71,161,765 bp
  • T to C, chromosome 11 at 94,548,464 bp
  • T to A, chromosome 12 at 38,228,026 bp
  • T to A, chromosome 12 at 40,223,560 bp
  • T to C, chromosome 12 at 57,460,954 bp
  • C to T, chromosome 12 at 108,875,193 bp
  • A to G, chromosome 13 at 9,615,775 bp
  • T to C, chromosome 14 at 55,544,584 bp
  • C to T, chromosome 14 at 64,075,607 bp
  • T to C, chromosome 14 at 64,113,678 bp
  • C to T, chromosome 14 at 66,356,283 bp
  • T to A, chromosome 14 at 75,257,709 bp
  • T to A, chromosome 14 at 101,729,553 bp
  • T to C, chromosome 15 at 5,015,380 bp
  • A to T, chromosome 15 at 8,361,737 bp
  • T to C, chromosome 15 at 10,490,457 bp
  • A to C, chromosome 15 at 77,943,390 bp
  • T to G, chromosome 16 at 38,602,510 bp
  • A to T, chromosome 16 at 94,335,757 bp
  • A to G, chromosome 17 at 7,331,156 bp
  • T to A, chromosome 17 at 20,234,703 bp
  • C to G, chromosome 17 at 29,367,008 bp
  • A to G, chromosome 17 at 34,958,832 bp
  • T to A, chromosome 17 at 66,116,719 bp
  • A to G, chromosome 17 at 71,626,825 bp
  • A to T, chromosome 19 at 13,705,135 bp
  • T to A, chromosome 19 at 36,104,403 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0271 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038497-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.