Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR0277Btlr/Mmmh
Stock Number:
038499-MU
Citation ID:
RRID:MMRRC_038499-MU
Other Names:
R0277 (G1), C57BL/6J-MtgxR0277Btlr
Major Collection:

Strain Information

Zmpste24
Name: zinc metallopeptidase, STE24
Synonyms: A530043O15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230709
Homologene: 4277
Sort1
Name: sortilin 1
Synonyms: sortilin, neurotensin receptor 3, Ntsr3, Ntr3, 2900053A11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20661
Homologene: 136097
Adgre1
Name: adhesion G protein-coupled receptor E1
Synonyms: F4/80, DD7A5-7, TM7LN3, EGF-TM7, Ly71, Emr1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13733
VEGA: 17
HGNC: HGNC:3336
Homologene: 1493
Ndrg3
Name: N-myc downstream regulated gene 3
Synonyms: Ndr3, 4833415O14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29812
Homologene: 40903
Gldc
Name: glycine decarboxylase
Synonyms: D19Wsu57e, D030049L12Rik, b2b2679Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104174
VEGA: 19
HGNC: HGNC:4313
Homologene: 141
Serpinc1
Name: serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
Synonyms: antithrombin, ATIII, At-3, At3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11905
HGNC: HGNC:775
Homologene: 20139
Gnl3
Name: guanine nucleotide binding protein nucleolar 3
Synonyms: nucleostemin, NS
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 30877
VEGA: 14
Homologene: 56670
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,275,538 bp
  • T to C, chromosome 1 at 55,019,257 bp
  • A to C, chromosome 1 at 65,388,616 bp
  • A to T, chromosome 1 at 96,988,673 bp
  • A to G, chromosome 1 at 130,195,263 bp
  • T to A, chromosome 1 at 160,989,702 bp
  • A to G, chromosome 1 at 184,944,952 bp
  • T to A, chromosome 2 at 5,914,888 bp
  • A to T, chromosome 2 at 48,937,467 bp
  • A to G, chromosome 2 at 69,671,296 bp
  • A to T, chromosome 2 at 84,806,427 bp
  • T to A, chromosome 2 at 104,441,248 bp
  • A to G, chromosome 2 at 127,459,979 bp
  • A to G, chromosome 2 at 156,934,935 bp
  • T to A, chromosome 2 at 158,785,547 bp
  • T to A, chromosome 3 at 36,943,182 bp
  • T to A, chromosome 3 at 82,038,156 bp
  • T to A, chromosome 3 at 86,126,671 bp
  • T to A, chromosome 3 at 97,843,712 bp
  • C to T, chromosome 3 at 99,352,391 bp
  • T to C, chromosome 3 at 103,914,581 bp
  • T to C, chromosome 3 at 105,979,892 bp
  • T to C, chromosome 3 at 108,324,592 bp
  • T to A, chromosome 3 at 129,910,523 bp
  • T to A, chromosome 4 at 51,228,477 bp
  • T to C, chromosome 4 at 111,617,222 bp
  • T to C, chromosome 4 at 113,937,621 bp
  • T to C, chromosome 4 at 114,925,608 bp
  • A to G, chromosome 4 at 121,082,853 bp
  • A to G, chromosome 5 at 97,087,823 bp
  • T to C, chromosome 5 at 110,834,690 bp
  • A to G, chromosome 5 at 112,693,347 bp
  • C to T, chromosome 5 at 128,988,903 bp
  • G to T, chromosome 6 at 5,491,620 bp
  • T to A, chromosome 6 at 51,457,468 bp
  • A to T, chromosome 6 at 116,582,601 bp
  • T to C, chromosome 6 at 119,620,328 bp
  • T to A, chromosome 7 at 6,488,434 bp
  • T to C, chromosome 7 at 16,747,380 bp
  • A to T, chromosome 7 at 24,923,799 bp
  • G to T, chromosome 7 at 28,085,493 bp
  • C to G, chromosome 7 at 44,211,629 bp
  • A to T, chromosome 7 at 75,206,439 bp
  • C to T, chromosome 7 at 89,633,057 bp
  • C to A, chromosome 7 at 96,694,950 bp
  • A to G, chromosome 7 at 98,847,383 bp
  • A to G, chromosome 7 at 125,318,643 bp
  • T to G, chromosome 7 at 140,857,900 bp
  • T to C, chromosome 8 at 71,355,952 bp
  • T to A, chromosome 8 at 84,028,720 bp
  • T to G, chromosome 9 at 3,025,619 bp
  • T to C, chromosome 9 at 7,129,046 bp
  • G to A, chromosome 9 at 40,074,714 bp
  • G to A, chromosome 9 at 64,691,350 bp
  • A to T, chromosome 9 at 65,465,347 bp
  • T to C, chromosome 9 at 104,124,025 bp
  • T to A, chromosome 10 at 43,372,176 bp
  • A to G, chromosome 10 at 43,896,007 bp
  • T to C, chromosome 10 at 80,526,909 bp
  • T to C, chromosome 10 at 84,374,451 bp
  • T to C, chromosome 10 at 127,991,405 bp
  • T to A, chromosome 10 at 129,874,067 bp
  • A to C, chromosome 11 at 9,294,701 bp
  • A to G, chromosome 11 at 34,054,322 bp
  • T to A, chromosome 11 at 35,852,348 bp
  • A to T, chromosome 11 at 73,940,947 bp
  • G to A, chromosome 11 at 77,140,923 bp
  • A to G, chromosome 11 at 79,686,629 bp
  • A to T, chromosome 11 at 83,186,951 bp
  • A to C, chromosome 11 at 94,643,422 bp
  • A to G, chromosome 11 at 115,850,720 bp
  • A to G, chromosome 11 at 117,739,881 bp
  • A to T, chromosome 11 at 120,368,011 bp
  • G to A, chromosome 11 at 120,678,420 bp
  • A to G, chromosome 12 at 3,442,487 bp
  • A to T, chromosome 12 at 55,677,238 bp
  • A to G, chromosome 13 at 17,696,980 bp
  • G to T, chromosome 13 at 67,198,664 bp
  • T to C, chromosome 13 at 76,074,932 bp
  • T to A, chromosome 13 at 91,564,596 bp
  • A to G, chromosome 14 at 20,661,788 bp
  • A to T, chromosome 14 at 28,513,268 bp
  • A to C, chromosome 14 at 31,013,427 bp
  • T to C, chromosome 14 at 33,083,785 bp
  • T to C, chromosome 14 at 50,186,332 bp
  • T to C, chromosome 14 at 52,390,389 bp
  • T to C, chromosome 14 at 55,632,115 bp
  • C to A, chromosome 14 at 55,710,927 bp
  • T to C, chromosome 14 at 55,712,652 bp
  • T to C, chromosome 14 at 68,510,857 bp
  • T to A, chromosome 14 at 75,265,458 bp
  • A to T, chromosome 15 at 58,184,209 bp
  • C to A, chromosome 15 at 65,948,503 bp
  • C to A, chromosome 16 at 32,755,690 bp
  • A to T, chromosome 16 at 62,776,644 bp
  • G to A, chromosome 17 at 6,754,765 bp
  • T to C, chromosome 17 at 46,549,489 bp
  • T to A, chromosome 17 at 57,444,060 bp
  • A to C, chromosome 17 at 90,700,742 bp
  • A to G, chromosome 18 at 58,045,317 bp
  • A to G, chromosome 18 at 60,270,646 bp
  • C to T, chromosome 18 at 62,986,963 bp
  • A to G, chromosome 18 at 64,568,252 bp
  • A to T, chromosome 19 at 4,745,145 bp
  • A to T, chromosome 19 at 5,654,065 bp
  • A to G, chromosome 19 at 6,090,712 bp
  • A to T, chromosome 19 at 17,121,389 bp
  • A to G, chromosome 19 at 29,047,792 bp
  • A to G, chromosome 19 at 30,116,451 bp
  • A to T, chromosome 19 at 47,857,977 bp
  • T to A, chromosome Y at 1,169,979 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0277 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038499-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.