Strain Name:
C57BL/6J-MtgxR0281Btlr/Mmmh
Stock Number:
038503-MU
Citation ID:
RRID:MMRRC_038503-MU
Other Names:
R0281 (G1), C57BL/6J-MtgxR0281Btlr
Major Collection:

Gene Information

Gene Symbol: Kifc3 [MGI:109202] (Mus musculus (mouse))
Name: kinesin family member C3
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Mrtfb [MGI:3050795] (Mus musculus (mouse))
Name: myocardin related transcription factor B
Synonyms: MRTF-B, Mrtfb, Gt4-1, Mkl2
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Fezf2 [MGI:1859823] (Mus musculus (mouse))
Name: Fez family zinc finger 2
Synonyms: forebrain embryonic zinc finger, Fezl, Zfp312, Fez
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Snap25 [MGI:98331] (Mus musculus (mouse))
Name: synaptosomal-associated protein 25
Synonyms: SNAP-25, Bdr, GENA 70, sp
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Ly6a [MGI:107527] (Mus musculus (mouse))
Name: lymphocyte antigen 6 complex, locus A
Synonyms: Ly-6A/E, Sca1, Ly-6A.2, Ly-6E.1, Sca-1, TAP
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Itk [MGI:96621] (Mus musculus (mouse))
Name: IL2 inducible T cell kinase
Synonyms: Emt, Tsk, Tcsk
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Ptprk [MGI:103310] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type, K
Synonyms: RPTPkappa, PTPk
Chromosome: 10
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 74,927,071 bp
  • C to A, chromosome 1 at 75,492,927 bp
  • T to A, chromosome 1 at 79,435,512 bp
  • T to C, chromosome 1 at 97,716,553 bp
  • T to A, chromosome 1 at 100,072,153 bp
  • C to T, chromosome 1 at 138,832,904 bp
  • T to C, chromosome 2 at 22,245,598 bp
  • A to G, chromosome 2 at 29,179,643 bp
  • T to C, chromosome 2 at 34,774,320 bp
  • C to T, chromosome 2 at 88,050,412 bp
  • A to G, chromosome 2 at 112,686,810 bp
  • T to C, chromosome 2 at 121,270,237 bp
  • C to T, chromosome 2 at 122,292,304 bp
  • T to C, chromosome 2 at 128,782,621 bp
  • A to G, chromosome 2 at 136,777,464 bp
  • A to G, chromosome 2 at 165,296,890 bp
  • T to C, chromosome 3 at 27,222,804 bp
  • C to A, chromosome 3 at 27,457,006 bp
  • T to C, chromosome 3 at 40,785,408 bp
  • C to A, chromosome 3 at 107,331,155 bp
  • T to A, chromosome 3 at 108,095,562 bp
  • A to T, chromosome 4 at 58,784,981 bp
  • A to T, chromosome 4 at 62,546,067 bp
  • G to A, chromosome 4 at 136,271,366 bp
  • A to T, chromosome 4 at 143,350,409 bp
  • G to T, chromosome 4 at 147,755,265 bp
  • C to T, chromosome 5 at 5,736,431 bp
  • T to C, chromosome 5 at 24,566,564 bp
  • A to T, chromosome 5 at 52,150,746 bp
  • A to G, chromosome 5 at 120,674,605 bp
  • G to A, chromosome 5 at 121,254,251 bp
  • C to T, chromosome 5 at 137,296,127 bp
  • G to A, chromosome 6 at 83,267,567 bp
  • A to T, chromosome 6 at 84,574,556 bp
  • T to A, chromosome 7 at 16,140,989 bp
  • C to T, chromosome 7 at 29,706,571 bp
  • T to A, chromosome 7 at 33,615,005 bp
  • T to A, chromosome 7 at 35,619,371 bp
  • A to T, chromosome 7 at 79,100,285 bp
  • T to A, chromosome 7 at 85,233,249 bp
  • C to G, chromosome 7 at 85,233,258 bp
  • A to G, chromosome 7 at 86,694,860 bp
  • G to T, chromosome 7 at 105,407,668 bp
  • A to C, chromosome 7 at 108,346,914 bp
  • A to T, chromosome 8 at 33,429,379 bp
  • T to C, chromosome 8 at 71,511,382 bp
  • G to A, chromosome 8 at 95,071,253 bp
  • A to G, chromosome 8 at 95,103,460 bp
  • T to C, chromosome 8 at 122,895,568 bp
  • T to A, chromosome 8 at 128,460,683 bp
  • G to A, chromosome 9 at 37,523,219 bp
  • A to T, chromosome 9 at 58,145,489 bp
  • G to A, chromosome 9 at 59,554,226 bp
  • T to C, chromosome 9 at 95,937,566 bp
  • T to A, chromosome 9 at 105,784,116 bp
  • T to G, chromosome 9 at 108,498,509 bp
  • G to A, chromosome 9 at 120,131,003 bp
  • A to T, chromosome 10 at 28,573,392 bp
  • T to C, chromosome 10 at 52,350,678 bp
  • A to T, chromosome 10 at 127,205,821 bp
  • A to G, chromosome 10 at 128,474,722 bp
  • T to A, chromosome 10 at 129,874,546 bp
  • T to A, chromosome 11 at 34,069,854 bp
  • T to C, chromosome 11 at 43,153,304 bp
  • T to C, chromosome 11 at 46,353,916 bp
  • T to C, chromosome 11 at 58,206,054 bp
  • T to C, chromosome 11 at 58,440,012 bp
  • T to A, chromosome 11 at 59,038,615 bp
  • T to C, chromosome 11 at 72,317,530 bp
  • C to T, chromosome 11 at 78,271,924 bp
  • T to A, chromosome 11 at 93,882,177 bp
  • T to C, chromosome 11 at 95,511,256 bp
  • T to A, chromosome 11 at 97,021,925 bp
  • C to A, chromosome 11 at 97,806,851 bp
  • T to A, chromosome 11 at 101,074,027 bp
  • T to C, chromosome 12 at 11,457,042 bp
  • T to A, chromosome 13 at 21,072,374 bp
  • A to T, chromosome 13 at 70,604,047 bp
  • A to T, chromosome 13 at 113,369,143 bp
  • A to G, chromosome 14 at 12,343,977 bp
  • T to C, chromosome 14 at 47,289,868 bp
  • T to A, chromosome 14 at 66,037,606 bp
  • A to C, chromosome 14 at 78,510,089 bp
  • T to A, chromosome 15 at 12,257,706 bp
  • T to A, chromosome 15 at 34,529,858 bp
  • C to T, chromosome 15 at 74,995,387 bp
  • A to G, chromosome 15 at 78,250,883 bp
  • T to A, chromosome 16 at 13,412,163 bp
  • T to A, chromosome 16 at 20,422,400 bp
  • A to G, chromosome 16 at 21,914,157 bp
  • T to A, chromosome 16 at 25,764,302 bp
  • T to C, chromosome 17 at 23,401,413 bp
  • T to C, chromosome 17 at 31,455,106 bp
  • T to A, chromosome 17 at 32,213,540 bp
  • C to T, chromosome 17 at 46,412,614 bp
  • T to A, chromosome 17 at 56,885,169 bp
  • A to G, chromosome 17 at 67,817,569 bp
  • T to C, chromosome 18 at 5,094,582 bp
  • A to G, chromosome 18 at 35,800,132 bp
  • T to C, chromosome 18 at 36,737,704 bp
  • A to G, chromosome 18 at 67,828,482 bp
  • A to G, chromosome 19 at 13,619,485 bp
  • G to A, chromosome 19 at 31,945,814 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0281 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038503-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.