Strain Name:
C57BL/6J-MtgxR0322Btlr/Mmmh
Stock Number:
038532-MU
Citation ID:
RRID:MMRRC_038532-MU
Other Names:
R0322 (G1), C57BL/6J-MtgxR0322Btlr
Major Collection:

Gene Information

Tyr
Name: tyrosinase
Synonyms: skc35, Oca1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22173
Homologene: 30969
Mtmr3
Name: myotubularin related protein 3
Synonyms: FYVE-DSP1, ZFYVE10, 1700092A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74302
HGNC: HGNC:7451
Homologene: 23662
Dnmbp
Name: dynamin binding protein
Synonyms: Tuba, 2410003L07Rik, 2410003M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 71972
Homologene: 9061
Smg7
Name: Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Synonyms: 9430023P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226517
Homologene: 32235
Srrt
Name: serrate RNA effector molecule homolog (Arabidopsis)
Synonyms: Asr2, Ars2, 2810019G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 83701
Homologene: 9298
Cobl
Name: cordon-bleu WH2 repeat
Synonyms: C530045F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12808
Homologene: 9058
Tubgcp5
Name: tubulin, gamma complex associated protein 5
Synonyms: B130010C12Rik, GCP5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233276
Homologene: 14172
Myo18a
Name: myosin XVIIIA
Synonyms: MyoPDZ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 360013
Homologene: 7977
Mdm2
Name: transformed mouse 3T3 cell double minute 2
Synonyms: 1700007J15Rik, Mdm-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17246
HGNC: HGNC:6973
Homologene: 1793
Riox2
Name: ribosomal oxygenase 2
Synonyms: Mina, 1810047J07Rik, 3830408E23Rik, 2410057H13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 67014
Homologene: 12071
Eef1aknmt
Name: EEF1A lysine and N-terminal methyltransferase
Synonyms: Mettl13, 5630401D24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71449
Homologene: 32285
Slc6a3
Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
Synonyms: DAT, Dat1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13162
VEGA: 13
Homologene: 55547
Ubr4
Name: ubiquitin protein ligase E3 component n-recognin 4
Synonyms: LOC381562, A930005E13Rik, 1810009A16Rik, p600, D930005K06Rik, Zubr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69116
Homologene: 10804
Tecr
Name: trans-2,3-enoyl-CoA reductase
Synonyms: D17Ertd178e, SC2, A230102P12Rik, Gpsn2, 2410016D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 106529
HGNC: HGNC:4551
Homologene: 36231
Il7r
Name: interleukin 7 receptor
Synonyms: IL-7Ralpha, IL-7 receptor alpha chain, CD127
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16197
VEGA: 15
HGNC: HGNC:6024
Homologene: 1646
Pcdh20
Name: protocadherin 20
Synonyms: C630015B17Rik, PCDH13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219257
Homologene: 11277
Tia1
Name: cytotoxic granule-associated RNA binding protein 1
Synonyms: 2310050N03Rik, mTIA-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21841
Homologene: 20692
Bub1b
Name: BUB1B, mitotic checkpoint serine/threonine kinase
Synonyms: BUBR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12236
HGNC: HGNC:1149
Homologene: 933
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, 2610100B16Rik, Odz3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23965
Homologene: 22673
Gm9008
Name: predicted pseudogene 9008
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 668155
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: IDPm, Idh-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Insc
Name: INSC spindle orientation adaptor protein
Synonyms: Inscuteable, 3830422K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233752
Homologene: 78662
Tnfsf8
Name: tumor necrosis factor (ligand) superfamily, member 8
Synonyms: Cd30L, CD153, CD30LG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21949
Homologene: 950
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: 1110021D17Rik, Polydom, 4833413O10Rik, D430029O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 64817
Homologene: 23386
Adgrb3
Name: adhesion G protein-coupled receptor B3
Synonyms: A830096D10Rik, Bai3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210933
HGNC: HGNC:945
Homologene: 1289
Dll3
Name: delta like canonical Notch ligand 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13389
HGNC: HGNC:2909
Homologene: 7291
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, Heatr7b1, OTTMUSG00000020804
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Pnpla5
Name: patatin-like phospholipase domain containing 5
Synonyms: 4833426H19Rik, GS2L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 75772
VEGA: 15
Homologene: 13376
Ogdhl
Name: oxoglutarate dehydrogenase-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239017
VEGA: 14
Homologene: 56801
Ankhd1
Name: ankyrin repeat and KH domain containing 1
Synonyms: 1110004O12Rik, 4933432B13Rik, A530027J04Rik, 9130019P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 108857
Homologene: 87006
Chl1
Name: cell adhesion molecule L1-like
Synonyms: CALL, LICAM2, A530023M13Rik, close homolog of L1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12661
HGNC: HGNC:1939
Homologene: 21314
Adam34
Name: a disintegrin and metallopeptidase domain 34
Synonyms: testase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 252866
Homologene: 78104
Pcid2
Name: PCI domain containing 2
Synonyms: A730042J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234069
Homologene: 10168
Tmprss11f
Name: transmembrane protease, serine 11f
Synonyms: 4732406D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243083
Homologene: 65356
Gjc3
Name: gap junction protein, gamma 3
Synonyms: Cx29, Gje1, connexin 29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 118446
Homologene: 15399
Npc1l1
Name: NPC1 like intracellular cholesterol transporter 1
Synonyms: 9130221N23Rik, Niemann-Pick disease, type C1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237636
HGNC: HGNC:7898
Homologene: 56585
Mymk
Name: myomaker, myoblast fusion factor
Synonyms: Tmem8c, 1110002H13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66139
Homologene: 11925
Fbxo43
Name: F-box protein 43
Synonyms: early mitotic inhibitor 2, 4930533G20Rik, XErp1 homolog, Emi2, endogenous meiotic inhibitor 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 78803
VEGA: 15
Homologene: 66393
Mboat1
Name: membrane bound O-acyltransferase domain containing 1
Synonyms: 9130215M02Rik, Oact1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218121
Homologene: 57467
Sh3tc1
Name: SH3 domain and tetratricopeptide repeats 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231147
Homologene: 10360
Phyhip
Name: phytanoyl-CoA hydroxylase interacting protein
Synonyms: PAHX-AP1, PAHX-AP#1, C630010D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105653
Homologene: 8847
Cobll1
Name: Cobl-like 1
Synonyms: D430044D16Rik, Coblr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 319876
Homologene: 8933
9130401M01Rik
Name: RIKEN cDNA 9130401M01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 75758
Homologene: 12622
Stc1
Name: stanniocalcin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 20855
VEGA: 14
Homologene: 2374
Gm15964
Name: predicted gene 15964
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Itm2c
Name: integral membrane protein 2C
Synonyms: Bricd2c, BRI3, 3110038L02Rik, ITM3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 64294
HGNC: HGNC:6175
Homologene: 11186
Tbpl2
Name: TATA box binding protein like 2
Synonyms: LOC227606, Trf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227606
Homologene: 133152
Noxa1
Name: NADPH oxidase activator 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241275
Homologene: 18156
Ndufa8
Name: NADH:ubiquinone oxidoreductase subunit A8
Synonyms: 0610033L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68375
HGNC: HGNC:7692
Homologene: 40932
Olfr1193
Name: olfactory receptor 1193
Synonyms: GA_x6K02T2Q125-50154044-50154826, Olfr1194-ps1, MOR226-1, MOR230-9, GA_x6K02T2Q125-50158288-50158818
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329460
Homologene: 27297
Psmb4
Name: proteasome (prosome, macropain) subunit, beta type 4
Synonyms: Pros-27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 19172
HGNC: HGNC:9541
Homologene: 2090
1700112J05Rik
Name: RIKEN cDNA 1700112J05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68246
Olfr290
Name: olfactory receptor 290
Synonyms: MOR254-1, GA_x6K02T2NHDJ-11170115-11169168
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258411
Homologene: 83130
Vmn2r65
Name: vomeronasal 2, receptor 65
Synonyms: ENSMUSG00000070600
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100009609
Mfsd4b3-ps
Name: major facilitator superfamily domain containing 4B3, pseudogene
Synonyms: G630090E17Rik, Mfsd4b3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 100041085
Homologene: 136347
Gpc5
Name: glypican 5
Synonyms: A230034F01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 103978
HGNC: HGNC:4453
Homologene: 3285
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 25,221,748 bp
  • C to T, chromosome 1 at 85,907,030 bp
  • C to T, chromosome 1 at 88,230,680 bp
  • A to G, chromosome 1 at 152,849,873 bp
  • A to G, chromosome 1 at 162,544,176 bp
  • A to G, chromosome 2 at 24,094,979 bp
  • A to G, chromosome 2 at 25,092,554 bp
  • A to T, chromosome 2 at 27,067,406 bp
  • T to C, chromosome 2 at 36,036,622 bp
  • A to T, chromosome 2 at 65,102,098 bp
  • T to C, chromosome 2 at 88,678,667 bp
  • G to A, chromosome 2 at 118,639,618 bp
  • A to G, chromosome 3 at 94,886,091 bp
  • G to T, chromosome 4 at 58,057,996 bp
  • T to C, chromosome 4 at 63,834,166 bp
  • T to A, chromosome 4 at 139,422,418 bp
  • G to A, chromosome 5 at 35,706,561 bp
  • A to G, chromosome 5 at 77,007,190 bp
  • A to T, chromosome 5 at 86,591,416 bp
  • G to A, chromosome 5 at 137,296,608 bp
  • A to T, chromosome 5 at 137,957,498 bp
  • T to C, chromosome 6 at 76,496,418 bp
  • C to T, chromosome 6 at 86,420,387 bp
  • A to T, chromosome 6 at 103,701,883 bp
  • A to G, chromosome 7 at 28,296,368 bp
  • G to A, chromosome 7 at 55,814,978 bp
  • C to T, chromosome 7 at 80,098,257 bp
  • A to G, chromosome 7 at 84,916,313 bp
  • A to T, chromosome 7 at 84,946,548 bp
  • A to T, chromosome 7 at 87,492,917 bp
  • A to G, chromosome 7 at 114,792,265 bp
  • G to A, chromosome 8 at 13,090,775 bp
  • G to T, chromosome 8 at 43,651,921 bp
  • A to G, chromosome 8 at 48,236,912 bp
  • A to G, chromosome 8 at 83,572,243 bp
  • T to C, chromosome 10 at 39,947,530 bp
  • G to T, chromosome 10 at 117,702,204 bp
  • T to C, chromosome 11 at 4,487,505 bp
  • T to A, chromosome 11 at 6,229,042 bp
  • T to A, chromosome 11 at 12,267,072 bp
  • T to C, chromosome 11 at 77,829,800 bp
  • T to C, chromosome 13 at 30,232,080 bp
  • T to A, chromosome 13 at 73,560,926 bp
  • A to G, chromosome 14 at 32,337,577 bp
  • T to C, chromosome 14 at 69,029,409 bp
  • G to A, chromosome 14 at 70,463,396 bp
  • T to C, chromosome 14 at 88,468,947 bp
  • T to A, chromosome 14 at 115,399,151 bp
  • A to T, chromosome 15 at 9,510,215 bp
  • T to C, chromosome 15 at 36,152,192 bp
  • A to T, chromosome 15 at 58,025,372 bp
  • G to T, chromosome 15 at 84,120,719 bp
  • A to T, chromosome 16 at 59,489,389 bp
  • G to A, chromosome 16 at 91,623,037 bp
  • T to A, chromosome 18 at 36,658,008 bp
  • T to C, chromosome 19 at 43,854,846 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0322 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038532-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.