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Strain Name:
C57BL/6J-MtgxR0326Btlr/Mmmh
Stock Number:
038536-MU
Citation ID:
RRID:MMRRC_038536-MU
Other Names:
R0326 (G1), C57BL/6J-MtgxR0326Btlr
Major Collection:

Strain Information

Mon1b
Name: MON1 homolog B, secretory traffciking associated
Synonyms: 5033413H12Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 270096
Homologene: 69174
Ptch2
Name: patched 2
Synonyms: ptc2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19207
HGNC: HGNC:9586
Homologene: 37842
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Psmd13
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
Synonyms: S11, 26S proteasome subunit p40.5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23997
HGNC: HGNC:9558
Homologene: 2110
Cops4
Name: COP9 signalosome subunit 4
Synonyms: COP9 complex S4, D5Ertd774e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 26891
Homologene: 8067
Chd1
Name: chromodomain helicase DNA binding protein 1
Synonyms: 4930525N21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12648
HGNC: HGNC:1915
Homologene: 68174
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, C030045D06Rik, Depdc2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109294
Homologene: 23523
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 11,285,065 bp
  • A to T, chromosome 1 at 24,285,051 bp
  • T to C, chromosome 1 at 59,180,583 bp
  • A to C, chromosome 1 at 98,490,634 bp
  • C to T, chromosome 1 at 130,742,898 bp
  • A to T, chromosome 2 at 26,060,333 bp
  • T to A, chromosome 2 at 31,423,225 bp
  • T to A, chromosome 2 at 76,737,495 bp
  • T to C, chromosome 2 at 76,743,122 bp
  • A to G, chromosome 2 at 80,553,370 bp
  • A to T, chromosome 2 at 113,405,363 bp
  • A to G, chromosome 2 at 145,919,955 bp
  • A to T, chromosome 2 at 180,182,426 bp
  • A to C, chromosome 3 at 36,592,376 bp
  • A to T, chromosome 3 at 53,651,938 bp
  • A to C, chromosome 3 at 88,762,548 bp
  • A to G, chromosome 3 at 94,870,113 bp
  • A to T, chromosome 3 at 98,853,274 bp
  • A to T, chromosome 3 at 110,135,503 bp
  • A to G, chromosome 4 at 43,654,378 bp
  • A to T, chromosome 4 at 99,831,394 bp
  • A to C, chromosome 4 at 108,272,253 bp
  • G to A, chromosome 4 at 117,108,884 bp
  • T to C, chromosome 4 at 118,161,706 bp
  • A to T, chromosome 4 at 118,829,825 bp
  • A to C, chromosome 4 at 132,242,352 bp
  • T to C, chromosome 4 at 143,439,203 bp
  • A to G, chromosome 4 at 145,288,459 bp
  • A to G, chromosome 4 at 147,883,288 bp
  • G to A, chromosome 5 at 33,408,847 bp
  • T to G, chromosome 5 at 100,528,542 bp
  • T to C, chromosome 5 at 105,910,275 bp
  • G to A, chromosome 5 at 110,652,375 bp
  • A to C, chromosome 5 at 114,123,950 bp
  • A to T, chromosome 5 at 122,814,604 bp
  • T to C, chromosome 5 at 124,123,925 bp
  • C to T, chromosome 5 at 143,967,128 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • T to C, chromosome 6 at 4,537,838 bp
  • T to A, chromosome 6 at 39,497,110 bp
  • A to T, chromosome 6 at 55,898,243 bp
  • A to T, chromosome 6 at 92,858,057 bp
  • T to C, chromosome 6 at 141,559,773 bp
  • G to A, chromosome 7 at 17,044,400 bp
  • T to A, chromosome 7 at 43,776,519 bp
  • T to A, chromosome 7 at 44,319,170 bp
  • C to T, chromosome 7 at 44,979,454 bp
  • T to A, chromosome 7 at 45,008,751 bp
  • A to G, chromosome 7 at 81,024,889 bp
  • T to C, chromosome 7 at 109,460,172 bp
  • T to G, chromosome 7 at 120,224,419 bp
  • T to G, chromosome 7 at 126,583,045 bp
  • T to G, chromosome 7 at 127,573,903 bp
  • T to A, chromosome 7 at 127,927,176 bp
  • T to A, chromosome 7 at 128,198,378 bp
  • T to C, chromosome 7 at 140,897,711 bp
  • A to G, chromosome 8 at 91,409,527 bp
  • T to A, chromosome 8 at 113,637,743 bp
  • C to T, chromosome 8 at 122,571,922 bp
  • T to C, chromosome 9 at 44,513,281 bp
  • T to A, chromosome 9 at 63,619,162 bp
  • C to A, chromosome 9 at 99,474,385 bp
  • C to A, chromosome 9 at 120,951,712 bp
  • T to A, chromosome 10 at 78,042,599 bp
  • T to G, chromosome 10 at 81,322,662 bp
  • A to T, chromosome 10 at 83,283,746 bp
  • A to T, chromosome 10 at 102,388,704 bp
  • A to G, chromosome 10 at 127,716,297 bp
  • A to G, chromosome 10 at 129,705,769 bp
  • A to C, chromosome 11 at 29,461,021 bp
  • A to G, chromosome 11 at 33,554,362 bp
  • A to G, chromosome 11 at 48,808,260 bp
  • T to C, chromosome 11 at 49,677,436 bp
  • A to G, chromosome 11 at 58,316,347 bp
  • T to C, chromosome 11 at 83,514,539 bp
  • G to T, chromosome 11 at 97,021,535 bp
  • T to A, chromosome 11 at 98,028,374 bp
  • C to T, chromosome 12 at 7,990,307 bp
  • T to C, chromosome 12 at 69,635,685 bp
  • G to T, chromosome 13 at 38,192,870 bp
  • T to C, chromosome 13 at 70,779,611 bp
  • T to C, chromosome 13 at 81,474,993 bp
  • A to G, chromosome 13 at 94,358,563 bp
  • C to T, chromosome 14 at 19,814,532 bp
  • T to A, chromosome 14 at 29,045,644 bp
  • A to G, chromosome 14 at 120,922,223 bp
  • G to A, chromosome 15 at 6,418,316 bp
  • A to G, chromosome 15 at 55,047,460 bp
  • G to A, chromosome 15 at 73,092,826 bp
  • T to C, chromosome 16 at 14,218,880 bp
  • T to C, chromosome 16 at 32,979,500 bp
  • T to A, chromosome 16 at 46,153,883 bp
  • T to A, chromosome 16 at 56,260,485 bp
  • A to T, chromosome 17 at 15,768,566 bp
  • A to T, chromosome 17 at 15,768,568 bp
  • A to T, chromosome 17 at 24,231,810 bp
  • A to G, chromosome 17 at 32,820,726 bp
  • A to T, chromosome 17 at 33,950,748 bp
  • A to T, chromosome 17 at 34,028,950 bp
  • A to G, chromosome 17 at 34,698,179 bp
  • A to G, chromosome 17 at 50,966,736 bp
  • G to T, chromosome 17 at 66,080,821 bp
  • C to T, chromosome 19 at 12,036,161 bp
  • C to T, chromosome 19 at 13,919,509 bp
  • T to A, chromosome 19 at 39,042,009 bp
  • T to A, chromosome 19 at 43,825,947 bp
  • C to A, chromosome 19 at 53,864,165 bp
  • A to T, chromosome Y at 1,263,321 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0326 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038536-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.