Strain Name:
C57BL/6J-MtgxR0345Btlr/Mmmh
Stock Number:
038552-MU
Citation ID:
RRID:MMRRC_038552-MU
Other Names:
R0345 (G1), C57BL/6J-MtgxR0345Btlr
Major Collection:

Gene Information

Gene Symbol: Chrnb4 [MGI:87892] (Mus musculus (mouse))
Name: cholinergic receptor, nicotinic, beta polypeptide 4
Synonyms: Acrb-4, Acrb4
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Ptprz1 [MGI:97816] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, Ptpz, phosphacan, Rptpbeta, Ptprz, RPTPz, PTPbeta, PTPzeta
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Prdm16 [MGI:1917923] (Mus musculus (mouse))
Name: PR domain containing 16
Synonyms: csp1, line 27, Mel1, 5730557K01Rik
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Sntb2 [MGI:101771] (Mus musculus (mouse))
Name: syntrophin, basic 2
Synonyms: Snt2
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Llgl2 [MGI:1918843] (Mus musculus (mouse))
Name: LLGL2 scribble cell polarity complex component
Synonyms: 9130006H11Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Ubr1 [MGI:1277977] (Mus musculus (mouse))
Name: ubiquitin protein ligase E3 component n-recognin 1
Synonyms: E3 alpha
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Ap2a2 [MGI:101920] (Mus musculus (mouse))
Name: adaptor-related protein complex 2, alpha 2 subunit
Synonyms: Adtab, alpha-adaptin C, alpha-C adaptin, L25, 2410074K14Rik
Chromosome: 7
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • GCCACCA to GCCA, chromosome 2 at 52,110,324 bp
  • G to T, chromosome 2 at 80,544,977 bp
  • T to C, chromosome 2 at 85,400,341 bp
  • A to C, chromosome 2 at 92,946,067 bp
  • T to C, chromosome 2 at 120,904,103 bp
  • T to A, chromosome 2 at 153,411,752 bp
  • T to C, chromosome 3 at 104,895,809 bp
  • T to C, chromosome 3 at 106,122,439 bp
  • T to C, chromosome 3 at 142,608,183 bp
  • A to T, chromosome 4 at 41,428,413 bp
  • T to C, chromosome 4 at 42,851,116 bp
  • A to G, chromosome 4 at 108,266,407 bp
  • A to T, chromosome 4 at 133,486,211 bp
  • A to T, chromosome 4 at 145,117,625 bp
  • T to C, chromosome 4 at 154,341,111 bp
  • C to T, chromosome 5 at 36,027,874 bp
  • G to A, chromosome 6 at 4,718,019 bp
  • A to G, chromosome 6 at 23,016,165 bp
  • T to C, chromosome 6 at 73,021,257 bp
  • T to C, chromosome 6 at 121,638,272 bp
  • T to C, chromosome 7 at 43,351,944 bp
  • C to T, chromosome 7 at 45,259,248 bp
  • C to T, chromosome 7 at 67,735,821 bp
  • T to A, chromosome 7 at 80,247,336 bp
  • T to A, chromosome 7 at 104,960,181 bp
  • T to C, chromosome 7 at 106,818,701 bp
  • A to T, chromosome 7 at 127,708,463 bp
  • T to A, chromosome 7 at 141,631,293 bp
  • T to C, chromosome 8 at 107,001,538 bp
  • C to T, chromosome 8 at 123,304,813 bp
  • T to A, chromosome 9 at 42,384,218 bp
  • A to G, chromosome 9 at 55,035,594 bp
  • A to G, chromosome 9 at 75,297,419 bp
  • A to G, chromosome 10 at 63,566,840 bp
  • A to G, chromosome 10 at 127,087,895 bp
  • T to A, chromosome 11 at 53,372,881 bp
  • C to A, chromosome 11 at 71,123,675 bp
  • T to C, chromosome 11 at 100,757,681 bp
  • A to G, chromosome 11 at 115,849,992 bp
  • T to C, chromosome 11 at 119,918,046 bp
  • T to C, chromosome 11 at 121,038,186 bp
  • C to T, chromosome 12 at 4,748,351 bp
  • A to C, chromosome 13 at 34,076,637 bp
  • C to A, chromosome 14 at 41,061,946 bp
  • T to C, chromosome 14 at 59,139,630 bp
  • T to A, chromosome 14 at 101,876,877 bp
  • G to T, chromosome 15 at 7,289,994 bp
  • A to T, chromosome 15 at 66,020,305 bp
  • A to G, chromosome 15 at 74,543,349 bp
  • G to T, chromosome 15 at 76,177,167 bp
  • A to T, chromosome 15 at 80,372,462 bp
  • A to T, chromosome 15 at 82,689,774 bp
  • A to T, chromosome 16 at 37,288,308 bp
  • G to A, chromosome 16 at 59,167,906 bp
  • T to A, chromosome 17 at 23,610,082 bp
  • A to G, chromosome 17 at 56,000,097 bp
  • A to T, chromosome 17 at 66,559,545 bp
  • A to T, chromosome 19 at 38,024,345 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0345 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038552-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.