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Strain Name:
C57BL/6J-MtgxR0364Btlr/Mmmh
Stock Number:
038570-MU
Citation ID:
RRID:MMRRC_038570-MU
Other Names:
R0364 (G1), C57BL/6J-MtgxR0364Btlr
Major Collection:

Strain Information

Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Slk
Name: STE20-like kinase
Synonyms: 9A2, mSLK, Etk4, SLK, Stk2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20874
Homologene: 22515
Cryzl1
Name: crystallin zeta like 1
Synonyms: 2210407J23Rik, 2410006O11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66609
HGNC: HGNC:2420
Homologene: 3749
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Utp4
Name: UTP4 small subunit processome component
Synonyms: TEG-292, Tex292, Cirh1a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 21771
HGNC: HGNC:1983
Homologene: 40775
Epc2
Name: enhancer of polycomb homolog 2
Synonyms: D2Ertd694e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227867
Homologene: 32274
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 74,236,887 bp
  • T to C, chromosome 1 at 91,152,347 bp
  • A to G, chromosome 1 at 93,388,658 bp
  • T to C, chromosome 2 at 13,310,507 bp
  • T to C, chromosome 2 at 32,679,137 bp
  • T to A, chromosome 2 at 49,537,133 bp
  • A to T, chromosome 2 at 86,553,779 bp
  • G to A, chromosome 2 at 119,382,960 bp
  • T to G, chromosome 2 at 120,345,638 bp
  • C to T, chromosome 2 at 130,202,103 bp
  • T to G, chromosome 2 at 146,942,156 bp
  • T to C, chromosome 3 at 87,089,799 bp
  • A to T, chromosome 3 at 92,824,335 bp
  • C to A, chromosome 3 at 146,945,181 bp
  • T to G, chromosome 4 at 88,720,290 bp
  • C to A, chromosome 4 at 115,432,920 bp
  • C to A, chromosome 5 at 103,533,348 bp
  • T to A, chromosome 5 at 113,075,953 bp
  • A to T, chromosome 5 at 113,614,517 bp
  • G to T, chromosome 5 at 125,389,664 bp
  • G to A, chromosome 5 at 139,379,178 bp
  • G to A, chromosome 6 at 5,266,156 bp
  • T to C, chromosome 6 at 39,508,299 bp
  • A to G, chromosome 6 at 66,678,843 bp
  • A to G, chromosome 6 at 122,880,769 bp
  • T to A, chromosome 6 at 132,847,681 bp
  • G to A, chromosome 6 at 140,591,747 bp
  • A to C, chromosome 7 at 27,227,749 bp
  • G to A, chromosome 7 at 28,611,128 bp
  • A to G, chromosome 7 at 36,770,533 bp
  • C to A, chromosome 7 at 71,350,613 bp
  • A to G, chromosome 7 at 81,594,051 bp
  • A to G, chromosome 7 at 88,074,386 bp
  • G to T, chromosome 7 at 105,596,264 bp
  • G to A, chromosome 7 at 127,474,579 bp
  • C to T, chromosome 7 at 128,290,614 bp
  • A to G, chromosome 8 at 23,157,706 bp
  • A to T, chromosome 8 at 81,997,314 bp
  • T to C, chromosome 8 at 106,898,537 bp
  • A to G, chromosome 8 at 121,885,015 bp
  • G to A, chromosome 9 at 19,477,972 bp
  • A to G, chromosome 9 at 38,116,029 bp
  • A to T, chromosome 9 at 43,018,401 bp
  • A to T, chromosome 9 at 44,699,335 bp
  • A to G, chromosome 9 at 48,743,576 bp
  • T to C, chromosome 9 at 58,199,744 bp
  • A to G, chromosome 9 at 59,563,935 bp
  • A to T, chromosome 9 at 111,025,167 bp
  • A to G, chromosome 9 at 118,841,142 bp
  • T to C, chromosome 9 at 119,522,599 bp
  • T to A, chromosome 9 at 121,798,216 bp
  • C to A, chromosome 10 at 23,985,148 bp
  • G to A, chromosome 10 at 56,115,851 bp
  • T to C, chromosome 10 at 114,502,982 bp
  • A to T, chromosome 11 at 49,636,991 bp
  • G to A, chromosome 11 at 59,128,281 bp
  • A to G, chromosome 11 at 64,979,310 bp
  • A to C, chromosome 11 at 67,953,610 bp
  • T to C, chromosome 11 at 70,640,458 bp
  • G to A, chromosome 11 at 70,683,310 bp
  • T to A, chromosome 11 at 71,114,004 bp
  • A to T, chromosome 11 at 79,029,025 bp
  • A to T, chromosome 11 at 79,441,957 bp
  • C to T, chromosome 11 at 103,500,640 bp
  • G to T, chromosome 11 at 115,156,964 bp
  • T to A, chromosome 11 at 121,212,143 bp
  • A to G, chromosome 12 at 116,257,477 bp
  • G to T, chromosome 13 at 15,724,764 bp
  • A to T, chromosome 13 at 20,564,493 bp
  • T to C, chromosome 13 at 50,432,441 bp
  • A to C, chromosome 13 at 95,731,275 bp
  • A to G, chromosome 14 at 26,724,473 bp
  • A to T, chromosome 14 at 54,948,347 bp
  • G to A, chromosome 14 at 67,822,680 bp
  • A to G, chromosome 15 at 6,580,791 bp
  • T to C, chromosome 15 at 75,948,661 bp
  • T to C, chromosome 15 at 82,690,052 bp
  • A to T, chromosome 15 at 97,718,973 bp
  • T to A, chromosome 16 at 84,857,387 bp
  • G to A, chromosome 16 at 91,707,267 bp
  • T to C, chromosome 17 at 37,226,043 bp
  • T to A, chromosome 17 at 68,840,548 bp
  • T to A, chromosome 17 at 87,389,714 bp
  • C to T, chromosome 18 at 21,915,062 bp
  • C to A, chromosome 18 at 37,485,835 bp
  • A to T, chromosome 18 at 84,016,124 bp
  • A to T, chromosome 19 at 42,747,553 bp
  • T to A, chromosome 19 at 47,620,189 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0364 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038570-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.