Strain Name:
C57BL/6J-MtgxR0496Btlr/Mmmh
Stock Number:
038692-MU
Citation ID:
RRID:MMRRC_038692-MU
Other Names:
R0496 (G1), C57BL/6J-MtgxR0496Btlr
Major Collection:

Gene Information

Gene Symbol: Slit1 [MGI:1315203] (Mus musculus (mouse))
Name: slit guidance ligand 1
Synonyms: Slil1
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Ccn2 [MGI:95537] (Mus musculus (mouse))
Name: cellular communication network factor 2
Synonyms: hypertrophic chondrocyte-specific gene product 24, Fisp12, Ccn2, Ctgf, Hcs24
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Epha7 [MGI:95276] (Mus musculus (mouse))
Name: Eph receptor A7
Synonyms: Mdk1, Ebk, Hek11, Cek11, Ehk3, MDK1
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Cemip2 [MGI:1890373] (Mus musculus (mouse))
Name: cell migration inducing hyaluronidase 2
Synonyms: 3110012M15Rik, Tmem2
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Mta1 [MGI:2150037] (Mus musculus (mouse))
Name: metastasis associated 1
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Tns3 [MGI:2443012] (Mus musculus (mouse))
Name: tensin 3
Synonyms: TEM6, Tens1, F830010I22Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Fancd2 [MGI:2448480] (Mus musculus (mouse))
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 40,006,822 bp
  • T to C, chromosome 1 at 74,278,981 bp
  • T to C, chromosome 1 at 74,421,340 bp
  • G to A, chromosome 1 at 87,936,900 bp
  • T to A, chromosome 1 at 151,411,270 bp
  • G to A, chromosome 2 at 28,693,010 bp
  • A to G, chromosome 2 at 30,174,971 bp
  • A to T, chromosome 2 at 35,013,571 bp
  • C to T, chromosome 2 at 69,277,884 bp
  • A to T, chromosome 2 at 88,224,155 bp
  • G to A, chromosome 2 at 156,079,419 bp
  • A to G, chromosome 2 at 164,852,461 bp
  • G to T, chromosome 2 at 165,280,815 bp
  • A to G, chromosome 3 at 36,987,635 bp
  • A to G, chromosome 3 at 88,254,498 bp
  • T to A, chromosome 3 at 88,369,137 bp
  • A to T, chromosome 3 at 103,739,429 bp
  • A to G, chromosome 3 at 138,068,244 bp
  • G to T, chromosome 3 at 157,016,267 bp
  • T to A, chromosome 4 at 9,424,241 bp
  • T to A, chromosome 4 at 28,821,292 bp
  • G to A, chromosome 4 at 86,341,198 bp
  • A to G, chromosome 4 at 128,591,889 bp
  • A to T, chromosome 4 at 133,277,591 bp
  • A to C, chromosome 4 at 137,404,468 bp
  • A to T, chromosome 5 at 20,661,359 bp
  • T to A, chromosome 5 at 24,501,243 bp
  • T to C, chromosome 5 at 43,868,891 bp
  • G to T, chromosome 5 at 63,806,343 bp
  • T to A, chromosome 5 at 75,077,065 bp
  • CCG to CCGCG, chromosome 6 at 52,260,635 bp
  • G to A, chromosome 6 at 101,150,570 bp
  • T to C, chromosome 6 at 113,555,130 bp
  • T to A, chromosome 6 at 115,905,902 bp
  • A to T, chromosome 7 at 46,108,820 bp
  • G to A, chromosome 7 at 65,927,249 bp
  • T to C, chromosome 7 at 103,085,590 bp
  • C to T, chromosome 7 at 103,611,997 bp
  • T to C, chromosome 7 at 131,146,131 bp
  • T to C, chromosome 8 at 11,510,224 bp
  • A to T, chromosome 8 at 71,682,397 bp
  • A to T, chromosome 8 at 85,109,980 bp
  • A to G, chromosome 9 at 7,155,180 bp
  • C to T, chromosome 9 at 21,419,548 bp
  • T to G, chromosome 9 at 44,509,518 bp
  • T to A, chromosome 9 at 79,777,285 bp
  • G to A, chromosome 9 at 110,039,850 bp
  • T to C, chromosome 9 at 111,241,556 bp
  • C to G, chromosome 10 at 4,090,006 bp
  • G to T, chromosome 10 at 24,597,515 bp
  • G to T, chromosome 10 at 24,672,052 bp
  • C to T, chromosome 10 at 59,879,801 bp
  • T to C, chromosome 10 at 62,993,170 bp
  • G to T, chromosome 10 at 63,421,602 bp
  • T to C, chromosome 10 at 75,511,266 bp
  • T to C, chromosome 10 at 95,541,344 bp
  • G to T, chromosome 10 at 128,086,174 bp
  • T to C, chromosome 10 at 128,276,509 bp
  • A to C, chromosome 11 at 8,547,262 bp
  • G to T, chromosome 11 at 8,929,430 bp
  • A to G, chromosome 11 at 9,291,701 bp
  • A to T, chromosome 11 at 66,075,135 bp
  • C to A, chromosome 11 at 67,348,815 bp
  • T to C, chromosome 11 at 69,035,507 bp
  • T to C, chromosome 11 at 73,964,880 bp
  • G to A, chromosome 11 at 77,473,041 bp
  • A to G, chromosome 11 at 80,100,356 bp
  • G to A, chromosome 11 at 80,183,950 bp
  • C to T, chromosome 11 at 100,012,329 bp
  • T to A, chromosome 12 at 57,457,177 bp
  • A to G, chromosome 12 at 76,038,940 bp
  • C to T, chromosome 12 at 113,131,321 bp
  • T to C, chromosome 13 at 6,568,714 bp
  • A to G, chromosome 13 at 94,472,938 bp
  • T to C, chromosome 13 at 114,853,899 bp
  • A to G, chromosome 14 at 33,140,738 bp
  • A to T, chromosome 14 at 51,803,532 bp
  • A to T, chromosome 14 at 52,004,907 bp
  • T to C, chromosome 14 at 67,817,518 bp
  • A to T, chromosome 15 at 99,639,832 bp
  • GT to G, chromosome 16 at 19,956,966 bp
  • G to A, chromosome 16 at 91,623,037 bp
  • T to C, chromosome 16 at 96,434,701 bp
  • A to G, chromosome 17 at 17,843,746 bp
  • A to G, chromosome 17 at 24,914,450 bp
  • G to A, chromosome 17 at 28,200,445 bp
  • A to G, chromosome 17 at 34,029,538 bp
  • A to G, chromosome 17 at 38,304,658 bp
  • A to G, chromosome 17 at 52,725,858 bp
  • A to G, chromosome 17 at 55,482,014 bp
  • A to G, chromosome 17 at 56,605,630 bp
  • A to G, chromosome 17 at 71,084,306 bp
  • A to G, chromosome 18 at 10,804,773 bp
  • A to G, chromosome 18 at 34,544,847 bp
  • T to C, chromosome 18 at 63,791,843 bp
  • A to T, chromosome 19 at 21,797,345 bp
  • A to G, chromosome 19 at 22,698,778 bp
  • G to A, chromosome 19 at 41,608,311 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0496 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038692-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.