Strain Name:
C57BL/6J-MtgxR0505Btlr/Mmmh
Stock Number:
038700-MU
Citation ID:
RRID:MMRRC_038700-MU
Other Names:
R0505 (G1), C57BL/6J-MtgxR0505Btlr
Major Collection:

Gene Information

Gene Symbol: Sbf2 [MGI:1921831] (Mus musculus (mouse))
Name: SET binding factor 2
Synonyms: SBF2, mMTMH1, 4833411B01Rik, Mtmr13, B430219L04Rik
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Bdnf [MGI:88145] (Mus musculus (mouse))
Name: brain derived neurotrophic factor
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Mrtfb [MGI:3050795] (Mus musculus (mouse))
Name: myocardin related transcription factor B
Synonyms: MRTF-B, Mrtfb, Gt4-1, Mkl2
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Ckm [MGI:88413] (Mus musculus (mouse))
Name: creatine kinase, muscle
Synonyms: M-CK, Ckmm, MCK
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Meis1 [MGI:104717] (Mus musculus (mouse))
Name: Meis homeobox 1
Synonyms: C530044H18Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Ebf2 [MGI:894332] (Mus musculus (mouse))
Name: early B cell factor 2
Synonyms: D14Ggc1e, Mmot1, O/E-3
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Med1 [MGI:1100846] (Mus musculus (mouse))
Name: mediator complex subunit 1
Synonyms: PBP, TRAP 220, l11Jus15, CRSP210, DRIP205, TRAP220, Pparbp
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 43,764,886 bp
  • A to G, chromosome 1 at 63,143,926 bp
  • T to C, chromosome 1 at 134,602,571 bp
  • C to T, chromosome 1 at 172,323,024 bp
  • AAAT to AAATTAAT, chromosome 1 at 182,279,729 bp
  • A to G, chromosome 1 at 194,644,348 bp
  • G to A, chromosome 2 at 14,310,032 bp
  • A to G, chromosome 2 at 22,962,504 bp
  • G to T, chromosome 2 at 25,434,894 bp
  • C to A, chromosome 2 at 52,110,737 bp
  • A to T, chromosome 2 at 76,849,991 bp
  • A to G, chromosome 2 at 85,353,749 bp
  • A to G, chromosome 2 at 109,675,343 bp
  • A to T, chromosome 2 at 111,329,328 bp
  • T to A, chromosome 2 at 118,431,036 bp
  • A to T, chromosome 2 at 121,269,969 bp
  • T to A, chromosome 2 at 127,532,867 bp
  • T to A, chromosome 2 at 150,193,080 bp
  • T to A, chromosome 2 at 153,870,952 bp
  • C to T, chromosome 2 at 162,947,335 bp
  • A to T, chromosome 2 at 167,644,987 bp
  • A to G, chromosome 3 at 28,120,822 bp
  • T to C, chromosome 3 at 72,960,296 bp
  • T to A, chromosome 3 at 87,745,699 bp
  • G to T, chromosome 3 at 88,051,217 bp
  • C to T, chromosome 3 at 94,956,355 bp
  • T to C, chromosome 3 at 108,980,204 bp
  • T to A, chromosome 3 at 115,122,681 bp
  • C to A, chromosome 3 at 127,573,238 bp
  • G to A, chromosome 3 at 146,014,380 bp
  • T to A, chromosome 4 at 59,020,438 bp
  • T to A, chromosome 4 at 103,155,623 bp
  • T to C, chromosome 4 at 109,840,403 bp
  • T to C, chromosome 4 at 120,931,095 bp
  • A to T, chromosome 4 at 153,996,304 bp
  • G to A, chromosome 5 at 36,816,198 bp
  • A to G, chromosome 5 at 50,009,334 bp
  • C to A, chromosome 5 at 61,809,567 bp
  • T to A, chromosome 5 at 63,805,111 bp
  • G to A, chromosome 5 at 100,452,293 bp
  • A to T, chromosome 6 at 32,202,119 bp
  • C to T, chromosome 6 at 38,332,664 bp
  • A to G, chromosome 6 at 59,353,387 bp
  • T to A, chromosome 6 at 88,090,242 bp
  • T to A, chromosome 6 at 142,353,562 bp
  • T to A, chromosome 7 at 6,239,075 bp
  • T to C, chromosome 7 at 7,133,024 bp
  • A to T, chromosome 7 at 19,419,452 bp
  • A to T, chromosome 7 at 66,290,908 bp
  • T to A, chromosome 7 at 102,724,029 bp
  • T to C, chromosome 7 at 103,732,376 bp
  • A to T, chromosome 7 at 110,399,343 bp
  • C to A, chromosome 8 at 4,260,532 bp
  • C to T, chromosome 8 at 15,992,758 bp
  • G to T, chromosome 8 at 40,755,224 bp
  • G to A, chromosome 8 at 48,341,160 bp
  • C to T, chromosome 8 at 72,662,337 bp
  • T to A, chromosome 8 at 83,295,307 bp
  • G to T, chromosome 8 at 83,934,650 bp
  • A to G, chromosome 8 at 85,047,362 bp
  • A to T, chromosome 9 at 7,272,929 bp
  • A to G, chromosome 9 at 18,385,895 bp
  • G to A, chromosome 9 at 37,416,759 bp
  • G to A, chromosome 9 at 65,703,462 bp
  • A to G, chromosome 9 at 107,572,071 bp
  • A to G, chromosome 9 at 119,172,034 bp
  • C to T, chromosome 10 at 20,438,746 bp
  • T to G, chromosome 10 at 75,585,957 bp
  • T to C, chromosome 10 at 75,897,066 bp
  • A to G, chromosome 10 at 88,091,359 bp
  • T to C, chromosome 10 at 89,791,443 bp
  • T to C, chromosome 10 at 127,183,697 bp
  • C to T, chromosome 10 at 127,457,700 bp
  • T to A, chromosome 11 at 6,339,936 bp
  • T to C, chromosome 11 at 9,291,058 bp
  • T to A, chromosome 11 at 19,011,360 bp
  • A to T, chromosome 11 at 54,625,963 bp
  • T to C, chromosome 11 at 70,690,199 bp
  • G to A, chromosome 11 at 98,156,904 bp
  • A to G, chromosome 11 at 103,503,025 bp
  • T to C, chromosome 11 at 104,528,288 bp
  • T to A, chromosome 12 at 70,959,964 bp
  • T to C, chromosome 12 at 76,099,464 bp
  • A to T, chromosome 12 at 88,371,691 bp
  • T to A, chromosome 12 at 99,719,035 bp
  • A to C, chromosome 12 at 101,885,672 bp
  • A to G, chromosome 12 at 118,106,510 bp
  • A to G, chromosome 13 at 4,576,307 bp
  • T to A, chromosome 13 at 55,004,954 bp
  • C to A, chromosome 13 at 67,102,119 bp
  • C to A, chromosome 14 at 33,009,139 bp
  • A to T, chromosome 14 at 67,371,736 bp
  • C to T, chromosome 14 at 68,124,159 bp
  • A to C, chromosome 14 at 87,090,964 bp
  • A to T, chromosome 14 at 110,749,932 bp
  • T to C, chromosome 14 at 120,942,733 bp
  • A to T, chromosome 15 at 4,994,142 bp
  • A to G, chromosome 15 at 27,767,907 bp
  • A to T, chromosome 15 at 44,589,418 bp
  • A to T, chromosome 15 at 75,975,256 bp
  • T to C, chromosome 16 at 13,126,467 bp
  • C to T, chromosome 16 at 13,412,526 bp
  • T to A, chromosome 16 at 16,037,667 bp
  • A to G, chromosome 16 at 22,089,099 bp
  • T to A, chromosome 16 at 23,458,138 bp
  • C to T, chromosome 16 at 60,205,732 bp
  • A to T, chromosome 17 at 6,941,727 bp
  • A to T, chromosome 17 at 20,462,834 bp
  • A to T, chromosome 17 at 23,882,594 bp
  • C to T, chromosome 17 at 29,676,285 bp
  • T to G, chromosome 17 at 42,592,953 bp
  • C to T, chromosome 17 at 86,944,578 bp
  • T to A, chromosome 18 at 46,715,611 bp
  • T to C, chromosome 19 at 4,914,654 bp
  • T to C, chromosome 19 at 12,884,079 bp
  • A to G, chromosome 19 at 12,884,546 bp
  • A to G, chromosome 19 at 18,873,902 bp
  • T to C, chromosome 19 at 41,324,564 bp
  • G to A, chromosome 19 at 41,953,734 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0505 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038700-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.