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Strain Name:
C57BL/6J-MtgxR0512Btlr/Mmmh
Stock Number:
038706-MU
Citation ID:
RRID:MMRRC_038706-MU
Other Names:
R0512 (G1), C57BL/6J-MtgxR0512Btlr
Major Collection:

Strain Information

Trp53
Name: transformation related protein 53
Synonyms: p53, p44
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22059
Homologene: 460
Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Nrxn2
Name: neurexin II
Synonyms: neurexin II beta, neurexin II alpha, 6430591O13Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18190
HGNC: HGNC:8009
Homologene: 86984
Magi1
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: WWP3, Gukmi1, AIP3, BAP1, Baiap1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14924
HGNC: HGNC:946
Homologene: 31257
Kdm4c
Name: lysine (K)-specific demethylase 4C
Synonyms: 2410141F18Rik, Jmjd2c
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76804
Homologene: 41004
Mfap4
Name: microfibrillar-associated protein 4
Synonyms: 1110007F23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76293
HGNC: HGNC:7035
Homologene: 134529
Tnrc6a
Name: trinucleotide repeat containing 6a
Synonyms: CAGH26, 3110054G10Rik, D130023A07Rik, 2010321I05Rik, Tnrc6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233833
Homologene: 41399
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 6,248,543 bp
  • A to G, chromosome 1 at 11,199,933 bp
  • T to C, chromosome 1 at 20,310,514 bp
  • A to T, chromosome 1 at 90,821,798 bp
  • T to C, chromosome 1 at 153,905,386 bp
  • G to A, chromosome 1 at 171,340,930 bp
  • C to T, chromosome 2 at 5,904,091 bp
  • T to A, chromosome 2 at 31,937,968 bp
  • GCCACCA to GCCA, chromosome 2 at 52,110,324 bp
  • T to C, chromosome 2 at 91,065,686 bp
  • T to C, chromosome 2 at 121,175,419 bp
  • C to T, chromosome 2 at 170,115,462 bp
  • A to T, chromosome 3 at 3,651,622 bp
  • A to G, chromosome 3 at 19,149,495 bp
  • T to C, chromosome 3 at 36,166,113 bp
  • T to A, chromosome 3 at 40,970,034 bp
  • T to G, chromosome 3 at 92,457,477 bp
  • T to C, chromosome 3 at 98,162,125 bp
  • A to G, chromosome 3 at 103,760,574 bp
  • A to G, chromosome 3 at 108,226,170 bp
  • C to T, chromosome 4 at 8,805,139 bp
  • T to A, chromosome 4 at 21,678,490 bp
  • A to G, chromosome 4 at 74,333,794 bp
  • T to A, chromosome 4 at 101,792,019 bp
  • A to C, chromosome 4 at 101,814,704 bp
  • T to A, chromosome 4 at 119,585,836 bp
  • T to A, chromosome 4 at 121,054,307 bp
  • A to G, chromosome 5 at 31,285,477 bp
  • T to C, chromosome 5 at 34,426,391 bp
  • T to G, chromosome 5 at 114,863,508 bp
  • A to G, chromosome 5 at 139,234,847 bp
  • A to T, chromosome 5 at 147,341,270 bp
  • A to G, chromosome 6 at 12,379,605 bp
  • T to A, chromosome 6 at 39,664,989 bp
  • CCG to CCGCG, chromosome 6 at 52,260,635 bp
  • T to A, chromosome 6 at 58,956,699 bp
  • A to G, chromosome 6 at 93,694,064 bp
  • A to T, chromosome 7 at 4,542,814 bp
  • A to G, chromosome 7 at 12,715,423 bp
  • A to G, chromosome 7 at 15,833,949 bp
  • A to G, chromosome 7 at 19,393,887 bp
  • A to T, chromosome 7 at 83,755,950 bp
  • A to G, chromosome 7 at 85,150,692 bp
  • A to G, chromosome 7 at 104,861,039 bp
  • A to G, chromosome 7 at 113,836,833 bp
  • T to A, chromosome 7 at 123,186,728 bp
  • C to T, chromosome 7 at 126,827,623 bp
  • T to A, chromosome 7 at 141,092,420 bp
  • T to G, chromosome 8 at 35,384,417 bp
  • C to A, chromosome 8 at 44,951,332 bp
  • G to A, chromosome 8 at 82,864,178 bp
  • A to G, chromosome 9 at 22,458,719 bp
  • C to A, chromosome 9 at 42,067,832 bp
  • A to T, chromosome 9 at 53,884,245 bp
  • A to G, chromosome 9 at 61,918,975 bp
  • A to G, chromosome 9 at 72,449,471 bp
  • T to C, chromosome 9 at 95,935,526 bp
  • T to C, chromosome 9 at 119,550,658 bp
  • A to G, chromosome 9 at 123,660,406 bp
  • T to C, chromosome 10 at 70,568,918 bp
  • A to G, chromosome 10 at 78,071,966 bp
  • T to A, chromosome 11 at 20,008,485 bp
  • T to C, chromosome 11 at 23,451,997 bp
  • A to T, chromosome 11 at 32,210,875 bp
  • T to A, chromosome 11 at 61,487,945 bp
  • A to G, chromosome 11 at 67,188,678 bp
  • T to A, chromosome 11 at 69,588,683 bp
  • A to G, chromosome 11 at 78,319,586 bp
  • C to A, chromosome 11 at 102,062,290 bp
  • A to T, chromosome 11 at 106,345,677 bp
  • C to A, chromosome 11 at 109,950,650 bp
  • A to T, chromosome 11 at 118,411,265 bp
  • A to G, chromosome 11 at 121,359,586 bp
  • A to T, chromosome 12 at 51,627,183 bp
  • A to T, chromosome 12 at 69,969,201 bp
  • G to T, chromosome 12 at 113,050,927 bp
  • A to T, chromosome 13 at 12,277,415 bp
  • T to C, chromosome 14 at 27,446,406 bp
  • G to T, chromosome 14 at 29,978,556 bp
  • C to A, chromosome 15 at 101,463,627 bp
  • A to G, chromosome 16 at 10,614,580 bp
  • T to C, chromosome 16 at 30,601,735 bp
  • A to G, chromosome 16 at 90,726,356 bp
  • C to A, chromosome 17 at 19,522,120 bp
  • G to A, chromosome 17 at 67,779,134 bp
  • T to G, chromosome 18 at 37,295,979 bp
  • A to G, chromosome 19 at 6,517,198 bp
  • A to G, chromosome 19 at 29,128,097 bp
  • A to G, chromosome 19 at 37,954,524 bp
  • A to C, chromosome 19 at 46,329,422 bp
  • C to A, chromosome X at 161,837,359 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0512 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038706-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.