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Strain Name:
C57BL/6J-MtgxR0522Btlr/Mmmh
Stock Number:
038715-MU
Citation ID:
RRID:MMRRC_038715-MU
Other Names:
R0522 (G1), C57BL/6J-MtgxR0522Btlr
Major Collection:

Strain Information

Slc26a5
Name: solute carrier family 26, member 5
Synonyms: Pres, prestin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Sult6b1
Name: sulfotransferase family, cytosolic, 6B, member 1
Synonyms: 2410078J06Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 73671
Homologene: 42242
Adgrl3
Name: adhesion G protein-coupled receptor L3
Synonyms: lectomedin 3, LEC3, D130075K09Rik, 5430402I23Rik, Lphn3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319387
Homologene: 22878
Cyp11b2
Name: cytochrome P450, family 11, subfamily b, polypeptide 2
Synonyms: aldosterone synthase, Cyp11b-2, Cyp11b, steroid-11-beta-hydroxylase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13072
Homologene: 128035
Trp53bp1
Name: transformation related protein 53 binding protein 1
Synonyms: 53BP1, p53BP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27223
Homologene: 4137
Lgals9
Name: lectin, galactose binding, soluble 9
Synonyms: LGALS35, galectin-9, gal-9, Lgals5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16859
Homologene: 134662
Rrp12
Name: ribosomal RNA processing 12 homolog
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107094
VEGA: 19
Homologene: 22370
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 34,537,153 bp
  • A to G, chromosome 1 at 34,579,570 bp
  • T to C, chromosome 1 at 88,071,392 bp
  • C to T, chromosome 1 at 88,218,249 bp
  • T to C, chromosome 1 at 164,211,763 bp
  • A to C, chromosome 1 at 181,059,747 bp
  • T to C, chromosome 1 at 184,731,640 bp
  • T to A, chromosome 2 at 3,511,049 bp
  • T to C, chromosome 2 at 25,363,277 bp
  • T to C, chromosome 2 at 60,479,515 bp
  • A to G, chromosome 2 at 118,284,012 bp
  • G to A, chromosome 2 at 120,030,341 bp
  • C to A, chromosome 2 at 121,251,868 bp
  • G to A, chromosome 2 at 160,574,677 bp
  • T to A, chromosome 3 at 55,128,365 bp
  • C to T, chromosome 3 at 87,800,872 bp
  • T to C, chromosome 3 at 87,847,109 bp
  • T to A, chromosome 3 at 133,466,804 bp
  • T to C, chromosome 4 at 11,519,416 bp
  • A to G, chromosome 4 at 19,824,010 bp
  • C to A, chromosome 4 at 32,672,837 bp
  • G to T, chromosome 4 at 42,957,083 bp
  • A to G, chromosome 4 at 46,649,806 bp
  • A to T, chromosome 4 at 86,445,103 bp
  • C to T, chromosome 4 at 138,222,161 bp
  • T to A, chromosome 5 at 6,772,014 bp
  • A to C, chromosome 5 at 21,846,345 bp
  • T to C, chromosome 5 at 81,726,801 bp
  • G to T, chromosome 5 at 114,950,688 bp
  • T to C, chromosome 5 at 115,300,506 bp
  • T to C, chromosome 6 at 85,621,615 bp
  • C to CAT, chromosome 6 at 116,042,870 bp
  • T to A, chromosome 7 at 27,518,195 bp
  • A to G, chromosome 7 at 43,589,253 bp
  • A to T, chromosome 7 at 44,981,779 bp
  • A to G, chromosome 7 at 79,460,151 bp
  • A to G, chromosome 7 at 100,121,383 bp
  • A to G, chromosome 7 at 100,395,222 bp
  • T to A, chromosome 8 at 22,088,797 bp
  • T to G, chromosome 8 at 27,449,958 bp
  • C to A, chromosome 8 at 71,682,274 bp
  • A to G, chromosome 8 at 83,730,176 bp
  • T to C, chromosome 8 at 117,614,288 bp
  • T to A, chromosome 9 at 107,655,213 bp
  • T to C, chromosome 10 at 40,857,612 bp
  • T to C, chromosome 10 at 76,090,700 bp
  • T to C, chromosome 10 at 76,321,531 bp
  • T to C, chromosome 10 at 81,636,355 bp
  • T to A, chromosome 10 at 88,431,466 bp
  • T to G, chromosome 10 at 103,161,777 bp
  • G to T, chromosome 11 at 78,965,812 bp
  • A to T, chromosome 11 at 87,086,462 bp
  • A to C, chromosome 11 at 97,692,047 bp
  • T to A, chromosome 13 at 42,304,954 bp
  • G to T, chromosome 13 at 43,059,591 bp
  • T to A, chromosome 13 at 81,528,442 bp
  • A to T, chromosome 14 at 37,094,000 bp
  • A to T, chromosome 15 at 74,851,684 bp
  • C to A, chromosome 15 at 78,615,785 bp
  • T to C, chromosome 15 at 82,022,592 bp
  • A to T, chromosome 15 at 97,806,679 bp
  • T to A, chromosome 15 at 99,280,519 bp
  • AAGAGAGAGAGAGAGA to AAGAGAGAGAGAGA, chromosome 16 at 36,915,205 bp
  • C to T, chromosome 17 at 43,038,213 bp
  • G to T, chromosome 17 at 55,896,201 bp
  • C to T, chromosome 17 at 78,905,529 bp
  • A to G, chromosome 19 at 12,461,759 bp
  • C to T, chromosome 19 at 41,874,705 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0522 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038715-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.