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Strain Name:
C57BL/6J-MtgxR0523Btlr/Mmmh
Stock Number:
038716-MU
Citation ID:
RRID:MMRRC_038716-MU
Other Names:
R0523 (G1), C57BL/6J-MtgxR0523Btlr
Major Collection:

Strain Information

Pax3
Name: paired box 3
Synonyms: Pax-3, Splchl2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494
Aggf1
Name: angiogenic factor with G patch and FHA domains 1
Synonyms: 2310029P06Rik, VG5Q, 2010009L17Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66549
Homologene: 41220
Dicer1
Name: dicer 1, ribonuclease type III
Synonyms: 1110006F08Rik, D12Ertd7e, Dicer1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 192119
VEGA: 12
Homologene: 13251
Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Pipox
Name: pipecolic acid oxidase
Synonyms: Pso
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19193
Homologene: 40640
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 37,644,629 bp
  • A to G, chromosome 1 at 78,195,441 bp
  • T to G, chromosome 1 at 192,130,436 bp
  • T to A, chromosome 2 at 60,522,755 bp
  • T to A, chromosome 2 at 85,769,929 bp
  • T to C, chromosome 2 at 104,789,676 bp
  • T to A, chromosome 2 at 110,884,855 bp
  • T to C, chromosome 2 at 118,442,096 bp
  • C to A, chromosome 2 at 121,251,868 bp
  • T to C, chromosome 2 at 122,456,416 bp
  • T to C, chromosome 2 at 130,044,662 bp
  • T to A, chromosome 2 at 140,389,566 bp
  • C to G, chromosome 2 at 157,224,036 bp
  • A to G, chromosome 3 at 69,025,888 bp
  • T to C, chromosome 3 at 75,081,858 bp
  • T to C, chromosome 3 at 87,457,792 bp
  • C to T, chromosome 3 at 98,070,970 bp
  • G to A, chromosome 3 at 98,111,598 bp
  • G to A, chromosome 3 at 118,899,203 bp
  • G to A, chromosome 4 at 59,719,716 bp
  • T to C, chromosome 4 at 109,456,922 bp
  • C to A, chromosome 4 at 119,241,530 bp
  • G to A, chromosome 4 at 147,826,462 bp
  • T to C, chromosome 4 at 152,379,519 bp
  • T to A, chromosome 4 at 156,045,361 bp
  • GCCTCCTCCTCCTCCTCC to GCCTCCTCCTCCTCC, chromosome 5 at 35,724,066 bp
  • T to A, chromosome 5 at 87,251,832 bp
  • G to T, chromosome 5 at 110,303,593 bp
  • T to C, chromosome 5 at 120,766,144 bp
  • T to C, chromosome 5 at 122,216,365 bp
  • T to C, chromosome 5 at 123,501,242 bp
  • C to A, chromosome 5 at 143,373,043 bp
  • T to C, chromosome 5 at 143,882,166 bp
  • G to T, chromosome 6 at 48,451,860 bp
  • T to A, chromosome 6 at 56,174,941 bp
  • A to T, chromosome 6 at 56,883,681 bp
  • C to T, chromosome 6 at 59,210,373 bp
  • T to A, chromosome 6 at 122,581,545 bp
  • T to C, chromosome 6 at 128,558,326 bp
  • A to G, chromosome 6 at 132,957,451 bp
  • A to T, chromosome 7 at 4,489,772 bp
  • A to G, chromosome 7 at 4,759,393 bp
  • G to A, chromosome 7 at 25,806,050 bp
  • G to A, chromosome 7 at 29,185,190 bp
  • A to G, chromosome 7 at 106,790,326 bp
  • A to T, chromosome 7 at 108,368,231 bp
  • T to G, chromosome 7 at 139,987,741 bp
  • T to A, chromosome 8 at 45,996,743 bp
  • G to A, chromosome 8 at 64,983,790 bp
  • T to C, chromosome 8 at 69,109,985 bp
  • G to C, chromosome 8 at 78,276,837 bp
  • T to C, chromosome 9 at 21,142,996 bp
  • T to C, chromosome 9 at 45,927,567 bp
  • A to T, chromosome 9 at 50,534,626 bp
  • C to G, chromosome 9 at 66,970,692 bp
  • A to T, chromosome 9 at 108,561,819 bp
  • C to T, chromosome 9 at 108,561,820 bp
  • T to C, chromosome 10 at 39,848,049 bp
  • T to G, chromosome 10 at 64,675,909 bp
  • T to C, chromosome 10 at 92,908,883 bp
  • A to G, chromosome 10 at 107,580,220 bp
  • A to T, chromosome 11 at 3,524,664 bp
  • T to G, chromosome 11 at 48,945,782 bp
  • T to C, chromosome 11 at 61,147,587 bp
  • A to G, chromosome 11 at 65,180,902 bp
  • T to C, chromosome 11 at 77,892,139 bp
  • T to A, chromosome 11 at 100,436,210 bp
  • A to G, chromosome 11 at 116,107,890 bp
  • A to C, chromosome 11 at 116,132,018 bp
  • T to A, chromosome 12 at 17,416,213 bp
  • C to A, chromosome 12 at 55,642,317 bp
  • A to G, chromosome 12 at 104,702,491 bp
  • T to C, chromosome 13 at 95,356,416 bp
  • A to G, chromosome 14 at 30,980,038 bp
  • T to A, chromosome 15 at 8,194,386 bp
  • T to C, chromosome 15 at 35,472,050 bp
  • A to G, chromosome 15 at 75,892,465 bp
  • T to C, chromosome 15 at 75,903,156 bp
  • A to G, chromosome 15 at 76,452,243 bp
  • T to G, chromosome 15 at 89,135,645 bp
  • G to T, chromosome 15 at 103,155,709 bp
  • T to A, chromosome 16 at 16,307,203 bp
  • G to A, chromosome 16 at 38,328,374 bp
  • T to A, chromosome 16 at 58,675,245 bp
  • A to T, chromosome 16 at 64,925,899 bp
  • T to C, chromosome 16 at 81,461,643 bp
  • T to C, chromosome 17 at 12,692,064 bp
  • T to A, chromosome 17 at 33,433,349 bp
  • G to T, chromosome 17 at 34,445,499 bp
  • C to T, chromosome 17 at 43,038,213 bp
  • G to A, chromosome 17 at 80,216,457 bp
  • C to T, chromosome 18 at 37,856,500 bp
  • T to C, chromosome 18 at 63,022,481 bp
  • T to A, chromosome 18 at 76,262,552 bp
  • T to C, chromosome 19 at 39,739,429 bp
  • T to A, chromosome 19 at 45,036,090 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0523 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038716-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.