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Strain Name:
C57BL/6J-MtgxR0525Btlr/Mmmh
Stock Number:
038718-MU
Citation ID:
RRID:MMRRC_038718-MU
Other Names:
R0525 (G1), C57BL/6J-MtgxR0525Btlr
Major Collection:

Strain Information

Grm1
Name: glutamate receptor, metabotropic 1
Synonyms: mGluR1, Grm1, Gprc1a, nmf373, rcw, 4930455H15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14816
HGNC: HGNC:4593
Homologene: 649
Arid5b
Name: AT-rich interaction domain 5B
Synonyms: 5430435G07Rik, Desrt, Mrf2beta, Mrf2alpha, Mrf2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71371
VEGA: 10
Homologene: 45872
Gfpt2
Name: glutamine fructose-6-phosphate transaminase 2
Synonyms: GFAT2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14584
HGNC: HGNC:4242
Homologene: 68439
Trp53bp1
Name: transformation related protein 53 binding protein 1
Synonyms: 53BP1, p53BP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27223
Homologene: 4137
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Mat2b
Name: methionine adenosyltransferase 2 non-catalytic beta subunit methionine
Synonyms: MATIIbeta, 2410018D16Rik, 1110064C04Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 108645
HGNC: HGNC:6905
Homologene: 8323
Triobp
Name: TRIO and F-actin binding protein
Synonyms: Mus EST 478828, Tara, EST478828
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110253
Homologene: 5104
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 44,206,382 bp
  • C to T, chromosome 1 at 88,218,249 bp
  • T to A, chromosome 1 at 93,271,974 bp
  • T to A, chromosome 1 at 106,946,702 bp
  • G to A, chromosome 1 at 172,239,688 bp
  • A to G, chromosome 2 at 25,794,268 bp
  • A to G, chromosome 2 at 30,281,028 bp
  • T to C, chromosome 2 at 36,850,190 bp
  • C to T, chromosome 2 at 86,356,275 bp
  • A to T, chromosome 2 at 87,309,349 bp
  • G to A, chromosome 2 at 88,767,314 bp
  • C to A, chromosome 2 at 121,251,868 bp
  • A to G, chromosome 2 at 155,640,478 bp
  • T to A, chromosome 3 at 17,785,529 bp
  • A to C, chromosome 3 at 107,297,939 bp
  • T to C, chromosome 4 at 96,579,565 bp
  • A to G, chromosome 4 at 100,441,520 bp
  • A to T, chromosome 4 at 109,242,264 bp
  • C to G, chromosome 4 at 124,851,766 bp
  • A to G, chromosome 4 at 135,764,926 bp
  • A to C, chromosome 4 at 155,855,595 bp
  • T to A, chromosome 5 at 5,742,903 bp
  • A to G, chromosome 5 at 108,654,615 bp
  • A to T, chromosome 5 at 138,192,616 bp
  • G to A, chromosome 6 at 85,587,760 bp
  • A to T, chromosome 6 at 119,926,564 bp
  • A to G, chromosome 6 at 122,629,980 bp
  • T to C, chromosome 7 at 13,102,161 bp
  • C to A, chromosome 7 at 25,740,883 bp
  • T to C, chromosome 7 at 119,928,754 bp
  • T to G, chromosome 7 at 120,465,810 bp
  • T to A, chromosome 7 at 125,331,499 bp
  • C to A, chromosome 8 at 22,079,077 bp
  • A to G, chromosome 8 at 45,253,049 bp
  • A to G, chromosome 8 at 47,082,443 bp
  • G to T, chromosome 8 at 83,268,985 bp
  • T to C, chromosome 8 at 110,748,283 bp
  • A to T, chromosome 8 at 114,151,652 bp
  • T to G, chromosome 9 at 39,647,471 bp
  • A to T, chromosome 10 at 10,719,209 bp
  • T to C, chromosome 10 at 68,097,846 bp
  • T to C, chromosome 10 at 88,091,400 bp
  • A to G, chromosome 11 at 3,262,545 bp
  • G to A, chromosome 11 at 9,293,371 bp
  • A to G, chromosome 11 at 40,682,669 bp
  • A to G, chromosome 11 at 49,829,775 bp
  • A to T, chromosome 11 at 62,715,244 bp
  • A to T, chromosome 11 at 96,956,098 bp
  • G to A, chromosome 11 at 101,763,928 bp
  • A to T, chromosome 11 at 102,839,253 bp
  • T to C, chromosome 12 at 69,168,911 bp
  • T to C, chromosome 12 at 104,238,339 bp
  • G to A, chromosome 12 at 105,698,965 bp
  • A to G, chromosome 13 at 67,112,258 bp
  • C to T, chromosome 13 at 91,760,067 bp
  • T to C, chromosome 15 at 78,973,898 bp
  • A to G, chromosome 15 at 79,713,447 bp
  • T to A, chromosome 16 at 5,217,861 bp
  • G to A, chromosome 16 at 21,540,109 bp
  • A to T, chromosome 16 at 37,129,797 bp
  • G to T, chromosome 16 at 44,259,446 bp
  • A to G, chromosome 16 at 49,132,051 bp
  • A to T, chromosome 16 at 91,686,245 bp
  • C to T, chromosome 17 at 43,038,213 bp
  • T to C, chromosome 17 at 56,017,873 bp
  • T to C, chromosome 17 at 56,126,630 bp
  • A to G, chromosome 17 at 63,863,647 bp
  • T to A, chromosome 17 at 71,799,098 bp
  • G to T, chromosome 17 at 73,545,081 bp
  • T to C, chromosome 19 at 34,319,327 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0525 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038718-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.