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Strain Name:
C57BL/6J-MtgxR0540Btlr/Mmmh
Stock Number:
038732-MU
Citation ID:
RRID:MMRRC_038732-MU
Other Names:
R0540 (G1), C57BL/6J-MtgxR0540Btlr
Major Collection:

Strain Information

Fga
Name: fibrinogen alpha chain
Synonyms: Fib, ENSMUSG00000059807
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14161
HGNC: HGNC:3661
Homologene: 428
Edn3
Name: endothelin 3
Synonyms: 114CH19, 114-CH19, tmgc48
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13616
HGNC: HGNC:3178
Homologene: 88
Mrtfb
Name: myocardin related transcription factor B
Synonyms: MRTF-B, Gt4-1, Mrtfb, Mkl2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239719
Homologene: 40917
Nr2f2
Name: nuclear receptor subfamily 2, group F, member 2
Synonyms: COUP-TFII, ARP-1, Tcfcoup2, Aporp1, COUP-TF2, 9430015G03Rik, EAR3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11819
HGNC: HGNC:7976
Homologene: 7628
Gpd2
Name: glycerol phosphate dehydrogenase 2, mitochondrial
Synonyms: Gdm1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14571
HGNC: HGNC:4456
Homologene: 352
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Prrc1
Name: proline-rich coiled-coil 1
Synonyms: 2310058D16Rik, 9430085A19Rik, 3110038B19Rik, 1190002C06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 73137
VEGA: 18
Homologene: 12491
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 107,079,232 bp
  • A to T, chromosome 1 at 152,855,962 bp
  • A to C, chromosome 1 at 161,251,103 bp
  • A to T, chromosome 1 at 182,492,184 bp
  • T to A, chromosome 2 at 23,192,515 bp
  • A to G, chromosome 2 at 57,364,338 bp
  • T to C, chromosome 2 at 73,571,471 bp
  • T to A, chromosome 2 at 80,642,640 bp
  • C to T, chromosome 2 at 84,725,591 bp
  • T to A, chromosome 2 at 92,375,637 bp
  • C to T, chromosome 2 at 104,233,445 bp
  • T to C, chromosome 2 at 122,126,669 bp
  • C to T, chromosome 2 at 138,283,816 bp
  • T to A, chromosome 2 at 150,190,713 bp
  • C to T, chromosome 2 at 158,186,294 bp
  • C to A, chromosome 2 at 174,760,974 bp
  • A to C, chromosome 3 at 28,650,159 bp
  • C to A, chromosome 3 at 55,207,683 bp
  • G to A, chromosome 3 at 58,555,652 bp
  • A to G, chromosome 3 at 60,019,206 bp
  • G to A, chromosome 3 at 83,028,562 bp
  • A to T, chromosome 3 at 92,620,328 bp
  • G to A, chromosome 3 at 95,133,007 bp
  • A to T, chromosome 3 at 106,822,513 bp
  • T to A, chromosome 3 at 152,248,242 bp
  • A to G, chromosome 4 at 11,252,926 bp
  • A to T, chromosome 4 at 64,020,455 bp
  • G to A, chromosome 4 at 87,841,044 bp
  • A to T, chromosome 4 at 112,773,027 bp
  • A to G, chromosome 4 at 120,921,981 bp
  • A to G, chromosome 4 at 123,315,930 bp
  • A to G, chromosome 4 at 135,863,868 bp
  • C to A, chromosome 4 at 156,222,747 bp
  • A to G, chromosome 5 at 35,568,759 bp
  • A to T, chromosome 5 at 66,283,634 bp
  • T to A, chromosome 5 at 76,324,982 bp
  • A to G, chromosome 5 at 77,007,271 bp
  • T to C, chromosome 5 at 94,962,980 bp
  • A to G, chromosome 5 at 110,830,931 bp
  • A to G, chromosome 5 at 114,748,274 bp
  • T to C, chromosome 5 at 115,588,956 bp
  • T to A, chromosome 5 at 124,451,310 bp
  • A to T, chromosome 5 at 129,754,845 bp
  • T to C, chromosome 5 at 136,974,597 bp
  • T to C, chromosome 6 at 7,871,193 bp
  • T to A, chromosome 6 at 12,331,542 bp
  • C to T, chromosome 6 at 55,679,587 bp
  • T to C, chromosome 6 at 76,902,430 bp
  • C to A, chromosome 6 at 77,244,628 bp
  • A to T, chromosome 6 at 86,675,594 bp
  • T to C, chromosome 6 at 122,472,533 bp
  • A to G, chromosome 7 at 5,884,097 bp
  • G to A, chromosome 7 at 26,081,711 bp
  • C to A, chromosome 7 at 70,354,712 bp
  • T to A, chromosome 7 at 81,545,017 bp
  • T to A, chromosome 7 at 98,071,946 bp
  • T to C, chromosome 7 at 99,588,196 bp
  • T to A, chromosome 7 at 105,720,788 bp
  • T to A, chromosome 7 at 107,097,726 bp
  • A to G, chromosome 7 at 107,845,569 bp
  • T to C, chromosome 7 at 108,924,285 bp
  • T to C, chromosome 8 at 3,851,265 bp
  • C to A, chromosome 8 at 70,115,159 bp
  • A to C, chromosome 9 at 7,051,480 bp
  • A to G, chromosome 9 at 49,024,060 bp
  • C to A, chromosome 9 at 67,860,777 bp
  • T to A, chromosome 9 at 72,307,965 bp
  • G to A, chromosome 9 at 72,844,848 bp
  • A to T, chromosome 9 at 107,545,298 bp
  • T to C, chromosome 9 at 110,247,392 bp
  • C to A, chromosome 9 at 110,895,784 bp
  • T to A, chromosome 10 at 41,922,695 bp
  • T to C, chromosome 10 at 79,886,969 bp
  • T to A, chromosome 10 at 89,023,944 bp
  • C to G, chromosome 11 at 49,134,722 bp
  • C to A, chromosome 11 at 49,215,400 bp
  • C to A, chromosome 11 at 50,969,843 bp
  • T to A, chromosome 11 at 58,831,972 bp
  • T to C, chromosome 11 at 59,375,543 bp
  • C to T, chromosome 11 at 68,597,710 bp
  • T to C, chromosome 11 at 69,344,358 bp
  • T to C, chromosome 11 at 116,848,369 bp
  • C to T, chromosome 11 at 120,715,162 bp
  • T to A, chromosome 12 at 16,682,193 bp
  • C to A, chromosome 12 at 83,059,804 bp
  • T to C, chromosome 12 at 85,933,676 bp
  • A to G, chromosome 12 at 103,419,067 bp
  • A to C, chromosome 12 at 118,082,511 bp
  • T to A, chromosome 13 at 21,573,778 bp
  • G to A, chromosome 13 at 45,557,530 bp
  • A to C, chromosome 13 at 111,763,510 bp
  • G to A, chromosome 13 at 117,232,750 bp
  • A to T, chromosome 14 at 20,660,680 bp
  • C to T, chromosome 14 at 105,499,033 bp
  • G to A, chromosome 15 at 10,591,621 bp
  • T to C, chromosome 15 at 78,287,908 bp
  • G to T, chromosome 16 at 10,542,562 bp
  • A to G, chromosome 16 at 13,381,601 bp
  • A to T, chromosome 16 at 15,632,115 bp
  • C to A, chromosome 16 at 19,957,014 bp
  • A to T, chromosome 16 at 29,348,942 bp
  • G to A, chromosome 16 at 31,838,174 bp
  • G to T, chromosome 16 at 35,272,227 bp
  • T to A, chromosome 16 at 45,166,977 bp
  • A to C, chromosome 16 at 97,744,175 bp
  • A to T, chromosome 17 at 17,538,554 bp
  • A to T, chromosome 17 at 24,621,712 bp
  • G to A, chromosome 17 at 33,886,647 bp
  • G to T, chromosome 17 at 33,999,500 bp
  • T to C, chromosome 17 at 34,645,359 bp
  • T to A, chromosome 17 at 34,671,918 bp
  • A to T, chromosome 17 at 44,099,495 bp
  • A to T, chromosome 17 at 55,665,765 bp
  • A to G, chromosome 17 at 78,946,456 bp
  • G to T, chromosome 18 at 36,640,280 bp
  • A to G, chromosome 18 at 37,145,889 bp
  • G to A, chromosome 18 at 57,374,550 bp
  • A to G, chromosome 18 at 62,951,896 bp
  • T to C, chromosome 19 at 4,941,911 bp
  • G to T, chromosome 19 at 10,533,318 bp
  • A to T, chromosome 19 at 33,559,567 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0540 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038732-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.