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Strain Name:
C57BL/6J-MtgxR0546Btlr/Mmmh
Stock Number:
038738-MU
Citation ID:
RRID:MMRRC_038738-MU
Other Names:
R0546 (G1), C57BL/6J-MtgxR0546Btlr
Major Collection:

Strain Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: 2610103K11Rik, D230017O13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268281
Homologene: 6489
Cyfip1
Name: cytoplasmic FMR1 interacting protein 1
Synonyms: P140SRA-1, Shyc, pl-1, l(7)1Rl, Sra-1, E030028J09Rik, l7Rl1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20430
Homologene: 22628
Sec23a
Name: SEC23 homolog A, COPII coat complex component
Synonyms: Sec23r, Msec23
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20334
Homologene: 4642
Zfhx3
Name: zinc finger homeobox 3
Synonyms: WBP9, A230102L03Rik, Atbf1, Sci
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Xpo4
Name: exportin 4
Synonyms: B430309A01Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 57258
Homologene: 10733
Gpatch2l
Name: G patch domain containing 2 like
Synonyms: 1700020O03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 70373
VEGA: 12
Homologene: 9942
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 36,195,971 bp
  • A to G, chromosome 1 at 58,250,174 bp
  • A to G, chromosome 2 at 76,745,519 bp
  • A to G, chromosome 2 at 82,258,920 bp
  • G to T, chromosome 2 at 83,803,242 bp
  • T to A, chromosome 2 at 86,639,229 bp
  • C to T, chromosome 2 at 86,657,891 bp
  • T to A, chromosome 2 at 87,990,472 bp
  • T to A, chromosome 2 at 109,999,421 bp
  • T to C, chromosome 2 at 151,970,812 bp
  • A to T, chromosome 4 at 45,904,597 bp
  • A to T, chromosome 4 at 101,635,191 bp
  • A to G, chromosome 4 at 137,502,294 bp
  • T to C, chromosome 5 at 7,975,870 bp
  • T to C, chromosome 5 at 31,257,601 bp
  • T to C, chromosome 5 at 100,404,070 bp
  • T to C, chromosome 5 at 105,120,970 bp
  • A to T, chromosome 5 at 108,174,964 bp
  • T to C, chromosome 5 at 123,999,051 bp
  • G to T, chromosome 5 at 130,254,078 bp
  • A to G, chromosome 6 at 23,077,077 bp
  • T to C, chromosome 6 at 30,580,963 bp
  • A to G, chromosome 6 at 52,777,696 bp
  • C to T, chromosome 7 at 44,904,708 bp
  • T to C, chromosome 7 at 48,643,515 bp
  • C to T, chromosome 7 at 55,922,816 bp
  • A to G, chromosome 7 at 103,470,700 bp
  • A to G, chromosome 7 at 109,921,426 bp
  • T to G, chromosome 7 at 111,508,141 bp
  • C to T, chromosome 7 at 127,310,314 bp
  • G to T, chromosome 7 at 140,131,678 bp
  • A to G, chromosome 7 at 140,955,743 bp
  • A to G, chromosome 8 at 11,557,031 bp
  • G to A, chromosome 8 at 40,826,431 bp
  • A to G, chromosome 8 at 46,103,630 bp
  • A to G, chromosome 8 at 69,612,231 bp
  • A to G, chromosome 8 at 83,355,721 bp
  • A to G, chromosome 8 at 105,932,334 bp
  • A to G, chromosome 8 at 108,794,187 bp
  • A to G, chromosome 8 at 110,970,106 bp
  • A to G, chromosome 8 at 122,358,489 bp
  • T to A, chromosome 9 at 66,068,777 bp
  • T to A, chromosome 9 at 92,595,335 bp
  • T to A, chromosome 10 at 11,183,887 bp
  • C to A, chromosome 10 at 80,899,323 bp
  • A to G, chromosome 10 at 125,230,873 bp
  • G to A, chromosome 10 at 129,874,538 bp
  • T to A, chromosome 11 at 42,162,601 bp
  • A to G, chromosome 11 at 48,845,509 bp
  • A to T, chromosome 11 at 50,815,630 bp
  • A to G, chromosome 11 at 60,506,313 bp
  • A to T, chromosome 11 at 73,297,187 bp
  • T to C, chromosome 11 at 87,083,613 bp
  • A to G, chromosome 11 at 98,999,226 bp
  • A to G, chromosome 11 at 100,580,723 bp
  • T to A, chromosome 11 at 109,433,954 bp
  • C to A, chromosome 12 at 35,928,330 bp
  • T to C, chromosome 12 at 58,985,167 bp
  • C to T, chromosome 12 at 80,178,434 bp
  • A to G, chromosome 12 at 86,288,848 bp
  • G to T, chromosome 13 at 36,204,586 bp
  • T to C, chromosome 13 at 69,739,300 bp
  • G to A, chromosome 13 at 92,368,184 bp
  • C to T, chromosome 14 at 31,139,550 bp
  • A to G, chromosome 14 at 57,613,274 bp
  • A to T, chromosome 15 at 58,558,408 bp
  • A to T, chromosome 16 at 4,085,807 bp
  • A to C, chromosome 16 at 14,205,628 bp
  • G to C, chromosome 16 at 34,879,475 bp
  • T to A, chromosome 16 at 36,452,257 bp
  • T to C, chromosome 17 at 24,580,138 bp
  • T to A, chromosome 17 at 37,924,338 bp
  • A to G, chromosome 18 at 46,291,072 bp
  • T to C, chromosome 18 at 65,306,717 bp
  • T to C, chromosome 19 at 17,020,666 bp
  • G to A, chromosome X at 163,979,301 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0546 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038738-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.