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Strain Name:
C57BL/6J-MtgxR0616Btlr/Mmmh
Stock Number:
038805-MU
Citation ID:
RRID:MMRRC_038805-MU
Other Names:
R0616 (G1), C57BL/6J-MtgxR0616Btlr
Major Collection:

Strain Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Npy2r
Name: neuropeptide Y receptor Y2
Synonyms: NPY-Y2 receptor
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18167
HGNC: HGNC:7957
Homologene: 701
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Utp20
Name: UTP20 small subunit processome component
Synonyms: mDRIM, DRIM, 3830408P06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Kcmf1
Name: potassium channel modulatory factor 1
Synonyms: clone DEBT-91, 1700094M07Rik, Pmcf
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74287
Homologene: 99845
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Depdc7
Name: DEP domain containing 7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 211896
Homologene: 16386
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 6,244,262 bp
  • A to G, chromosome 1 at 32,467,775 bp
  • G to T, chromosome 1 at 71,302,671 bp
  • G to A, chromosome 1 at 90,214,469 bp
  • C to T, chromosome 1 at 116,580,549 bp
  • T to C, chromosome 1 at 130,706,422 bp
  • T to C, chromosome 1 at 133,100,831 bp
  • T to C, chromosome 1 at 150,460,711 bp
  • T to C, chromosome 1 at 180,421,736 bp
  • T to C, chromosome 2 at 25,502,505 bp
  • G to A, chromosome 2 at 44,116,717 bp
  • A to T, chromosome 2 at 52,188,671 bp
  • T to C, chromosome 2 at 76,846,623 bp
  • A to G, chromosome 2 at 76,897,667 bp
  • T to A, chromosome 2 at 89,881,591 bp
  • T to A, chromosome 2 at 104,727,305 bp
  • G to A, chromosome 2 at 119,778,120 bp
  • A to T, chromosome 2 at 128,594,212 bp
  • T to C, chromosome 2 at 131,172,194 bp
  • A to G, chromosome 3 at 38,942,870 bp
  • A to G, chromosome 3 at 53,474,697 bp
  • T to A, chromosome 3 at 82,541,363 bp
  • A to G, chromosome 3 at 94,403,081 bp
  • A to T, chromosome 3 at 95,341,798 bp
  • T to C, chromosome 3 at 97,747,533 bp
  • G to A, chromosome 4 at 12,168,234 bp
  • A to G, chromosome 4 at 49,380,269 bp
  • T to C, chromosome 4 at 55,011,951 bp
  • G to T, chromosome 4 at 135,917,562 bp
  • T to C, chromosome 5 at 23,986,772 bp
  • A to G, chromosome 5 at 25,299,252 bp
  • T to C, chromosome 5 at 87,089,477 bp
  • A to G, chromosome 5 at 92,009,004 bp
  • C to T, chromosome 5 at 108,314,085 bp
  • T to A, chromosome 6 at 49,423,485 bp
  • T to C, chromosome 6 at 72,850,484 bp
  • A to G, chromosome 6 at 116,556,928 bp
  • T to C, chromosome 6 at 124,628,764 bp
  • T to C, chromosome 6 at 135,116,432 bp
  • A to T, chromosome 7 at 5,610,999 bp
  • A to G, chromosome 7 at 19,970,396 bp
  • A to G, chromosome 7 at 24,054,825 bp
  • T to C, chromosome 7 at 27,129,705 bp
  • A to G, chromosome 7 at 29,110,678 bp
  • T to C, chromosome 7 at 29,210,537 bp
  • A to G, chromosome 7 at 49,787,328 bp
  • A to G, chromosome 7 at 75,124,008 bp
  • A to T, chromosome 7 at 100,480,161 bp
  • A to G, chromosome 7 at 101,401,650 bp
  • T to A, chromosome 7 at 102,542,554 bp
  • A to T, chromosome 7 at 128,272,631 bp
  • A to G, chromosome 7 at 141,796,244 bp
  • A to G, chromosome 8 at 3,515,025 bp
  • A to G, chromosome 8 at 48,276,156 bp
  • A to G, chromosome 9 at 7,791,111 bp
  • G to A, chromosome 9 at 15,332,322 bp
  • G to A, chromosome 9 at 21,914,764 bp
  • G to T, chromosome 9 at 38,269,334 bp
  • A to T, chromosome 9 at 38,673,480 bp
  • G to A, chromosome 9 at 39,237,650 bp
  • A to T, chromosome 9 at 40,100,987 bp
  • A to G, chromosome 9 at 56,056,250 bp
  • T to G, chromosome 9 at 108,366,804 bp
  • G to A, chromosome 9 at 109,848,639 bp
  • C to T, chromosome 10 at 50,749,164 bp
  • A to T, chromosome 10 at 88,770,751 bp
  • T to C, chromosome 11 at 49,327,756 bp
  • T to C, chromosome 11 at 69,345,487 bp
  • C to T, chromosome 11 at 78,209,495 bp
  • T to G, chromosome 11 at 80,331,861 bp
  • T to C, chromosome 12 at 40,704,415 bp
  • C to T, chromosome 12 at 52,517,065 bp
  • C to A, chromosome 13 at 15,662,406 bp
  • T to A, chromosome 13 at 19,690,859 bp
  • C to A, chromosome 13 at 34,913,683 bp
  • T to C, chromosome 13 at 41,042,185 bp
  • A to G, chromosome 13 at 43,159,254 bp
  • A to G, chromosome 13 at 54,547,032 bp
  • C to G, chromosome 13 at 92,521,129 bp
  • G to T, chromosome 14 at 49,773,656 bp
  • G to A, chromosome 14 at 121,277,022 bp
  • C to A, chromosome 15 at 55,083,570 bp
  • A to T, chromosome 16 at 15,690,407 bp
  • A to T, chromosome 16 at 17,422,506 bp
  • C to T, chromosome 16 at 32,104,011 bp
  • A to T, chromosome 16 at 56,654,070 bp
  • A to G, chromosome 17 at 21,314,623 bp
  • A to G, chromosome 17 at 24,857,577 bp
  • A to T, chromosome 17 at 27,434,564 bp
  • A to C, chromosome 17 at 38,067,240 bp
  • T to A, chromosome 17 at 71,379,574 bp
  • A to T, chromosome 17 at 86,127,702 bp
  • T to C, chromosome 17 at 90,362,857 bp
  • A to T, chromosome 18 at 37,401,975 bp
  • A to T, chromosome 18 at 39,773,739 bp
  • T to C, chromosome 18 at 60,270,937 bp
  • G to A, chromosome 19 at 11,526,898 bp
  • A to G, chromosome 19 at 41,892,549 bp
  • G to A, chromosome X at 16,710,163 bp
  • T to C, chromosome X at 152,005,791 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0616 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038805-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.