Strain Name:
C57BL/6J-MtgxR0657Btlr/Mmmh
Stock Number:
038842-MU
Citation ID:
RRID:MMRRC_038842-MU
Other Names:
R0657 (G1), C57BL/6J-MtgxR0657Btlr
Major Collection:

Gene Information

Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Obt, Mt, Lda, Flo, Edy, Dz, Cycn, Whi, Todo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320790
Homologene: 19067
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Aldh7a1
Name: aldehyde dehydrogenase family 7, member A1
Synonyms: Atq1, D18Wsu181e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 110695
HGNC: HGNC:877
Homologene: 913
Ulk2
Name: unc-51 like kinase 2
Synonyms: Unc51.2, A830085I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 29869
Homologene: 5891
Slc17a5
Name: solute carrier family 17 (anion/sugar transporter), member 5
Synonyms: 4631416G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235504
Homologene: 56571
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12695
Homologene: 72199
Tars2
Name: threonyl-tRNA synthetase 2, mitochondrial (putative)
Synonyms: 2610024N01Rik, Tarsl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71807
Homologene: 23520
Iars
Name: isoleucine-tRNA synthetase
Synonyms: E430001P04Rik, 2510016L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105148
HGNC: HGNC:5330
Homologene: 5325
Ints8
Name: integrator complex subunit 8
Synonyms: D130008D20Rik, 2810013E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72656
Homologene: 9888
Trip12
Name: thyroid hormone receptor interactor 12
Synonyms: Gtl6, 1110036I07Rik, 6720416K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14897
Homologene: 44226
Huwe1
Name: HECT, UBA and WWE domain containing 1
Synonyms: Ib772, 5430439H10Rik, LOC382250, Ureb1, C430014N20Rik, Arf-bp1, Mule
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 59026
Homologene: 45994
Zzef1
Name: zinc finger, ZZ-type with EF hand domain 1
Synonyms: 8430405D05Rik, C130099L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 195018
Homologene: 9027
Pip4k2b
Name: phosphatidylinositol-5-phosphate 4-kinase, type II, beta
Synonyms: c11, PI5P4Kbeta, Pip5k2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 108083
HGNC: HGNC:8998
Homologene: 2634
Tmem135
Name: transmembrane protein 135
Synonyms: 2810439K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72759
Homologene: 11295
Itgb1
Name: integrin beta 1 (fibronectin receptor beta)
Synonyms: CD29, beta1 integrin, 4633401G24Rik, Fnrb, Gm9863
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16412
HGNC: HGNC:6153
Homologene: 22999
Btbd10
Name: BTB (POZ) domain containing 10
Synonyms: Gmrp1, 1110056N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68815
Homologene: 13009
Me2
Name: malic enzyme 2, NAD(+)-dependent, mitochondrial
Synonyms: D030040L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 107029
VEGA: 18
HGNC: HGNC:6984
Homologene: 37615
Hmgcll1
Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208982
Homologene: 27863
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Schnurri-2, Shn-2, Gm20114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Pde5a
Name: phosphodiesterase 5A, cGMP-specific
Synonyms: PDE5A1, Pde5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242202
HGNC: HGNC:8784
Homologene: 842
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Olfr394
Name: olfactory receptor 394
Synonyms: GA_x6K02T2P1NL-4043306-4042374, MOR135-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259009
Homologene: 133669
F13a1
Name: coagulation factor XIII, A1 subunit
Synonyms: Factor XIIIA, 1200014I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 74145
VEGA: 13
HGNC: HGNC:3531
Homologene: 20077
Mgat4b
Name: mannoside acetylglucosaminyltransferase 4, isoenzyme B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103534
HGNC: HGNC:7048
Homologene: 8611
Kif14
Name: kinesin family member 14
Synonyms: N-3 kinesin, D1Ertd367e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381293
Homologene: 8916
Bfsp1
Name: beaded filament structural protein 1, in lens-CP94
Synonyms: filensin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12075
HGNC: HGNC:1040
Homologene: 922
Defb13
Name: defensin beta 13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 246083
Homologene: 86849
4930503L19Rik
Name: RIKEN cDNA 4930503L19 gene
Synonyms: Las2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269033
Homologene: 52120
Neto1
Name: neuropilin (NRP) and tolloid (TLL)-like 1
Synonyms: C130005O10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 246317
VEGA: 18
Homologene: 16367
Nfatc2ip
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 interacting protein
Synonyms: NIP45, D7Ertd304e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18020
Homologene: 7862
Nek8
Name: NIMA (never in mitosis gene a)-related expressed kinase 8
Synonyms: 4632401F23Rik, b2b1449Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 140859
Homologene: 84442
Spata20
Name: spermatogenesis associated 20
Synonyms: Tisp78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217116
Homologene: 11264
Lypd10
Name: Ly6/PLAUR domain containing 10
Synonyms: BC049730
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232972
Hmgcs2
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2
Synonyms: mHS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 15360
HGNC: HGNC:5008
Homologene: 38066
F8
Name: coagulation factor VIII
Synonyms: Factor VIII, FVIII, Cf-8, Cf8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 14069
HGNC: HGNC:3546
Homologene: 49153
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 84,759,050 bp
  • C to A, chromosome 1 at 88,255,565 bp
  • C to T, chromosome 1 at 136,469,102 bp
  • A to C, chromosome 2 at 143,827,650 bp
  • A to T, chromosome 3 at 95,748,557 bp
  • A to G, chromosome 3 at 98,291,053 bp
  • A to G, chromosome 3 at 122,748,458 bp
  • T to C, chromosome 4 at 8,753,141 bp
  • C to A, chromosome 4 at 11,246,097 bp
  • C to A, chromosome 4 at 98,667,648 bp
  • T to A, chromosome 7 at 24,713,447 bp
  • A to T, chromosome 7 at 89,144,682 bp
  • A to T, chromosome 7 at 113,329,878 bp
  • A to G, chromosome 7 at 126,391,335 bp
  • T to C, chromosome 8 at 21,946,861 bp
  • T to G, chromosome 8 at 128,722,854 bp
  • A to T, chromosome 9 at 76,056,572 bp
  • G to A, chromosome 9 at 78,578,674 bp
  • T to C, chromosome 10 at 14,131,878 bp
  • T to C, chromosome 11 at 50,231,081 bp
  • T to C, chromosome 11 at 61,808,054 bp
  • T to C, chromosome 11 at 72,821,851 bp
  • T to C, chromosome 11 at 73,887,785 bp
  • C to T, chromosome 11 at 73,887,830 bp
  • C to T, chromosome 11 at 78,171,207 bp
  • T to A, chromosome 11 at 94,480,609 bp
  • A to T, chromosome 11 at 97,722,936 bp
  • A to T, chromosome 13 at 36,968,105 bp
  • T to C, chromosome 13 at 49,702,519 bp
  • A to G, chromosome 13 at 63,513,751 bp
  • C to T, chromosome 18 at 56,537,197 bp
  • G to A, chromosome 18 at 70,469,482 bp
  • A to G, chromosome 18 at 73,770,673 bp
  • G to A, chromosome 18 at 86,461,320 bp
  • T to C, chromosome X at 75,211,416 bp
  • T to C, chromosome X at 151,919,928 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0657 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038842-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.