MMRRC:038854-MU

A to T, chromosome 1 at 20,804,929 bp
A to G, chromosome 1 at 75,311,778 bp
T to G, chromosome 1 at 134,197,343 bp
T to C, chromosome 1 at 193,332,330 bp
T to G, chromosome 1 at 194,788,837 bp
A to G, chromosome 2 at 26,634,866 bp
T to C, chromosome 2 at 34,885,866 bp
C to A, chromosome 2 at 69,329,318 bp
A to T, chromosome 2 at 88,864,928 bp
A to G, chromosome 2 at 119,019,167 bp
T to C, chromosome 4 at 24,517,223 bp
T to A, chromosome 4 at 35,709,120 bp
A to T, chromosome 4 at 62,030,770 bp
C to T, chromosome 4 at 62,150,727 bp
A to T, chromosome 4 at 98,987,479 bp
A to G, chromosome 4 at 116,537,876 bp
T to C, chromosome 4 at 137,428,530 bp
A to G, chromosome 5 at 8,143,036 bp
A to G, chromosome 5 at 103,556,109 bp
T to C, chromosome 5 at 124,062,887 bp
C to T, chromosome 6 at 43,174,004 bp
T to C, chromosome 6 at 83,119,345 bp
A to T, chromosome 6 at 92,880,957 bp
T to C, chromosome 7 at 20,787,420 bp
C to A, chromosome 7 at 39,473,785 bp
T to C, chromosome 7 at 41,155,830 bp
T to C, chromosome 7 at 49,408,683 bp
T to C, chromosome 7 at 66,171,687 bp
A to T, chromosome 7 at 78,782,883 bp
A to G, chromosome 7 at 86,664,336 bp
A to C, chromosome 7 at 101,999,677 bp
T to A, chromosome 7 at 105,693,704 bp
T to A, chromosome 7 at 107,903,239 bp
T to A, chromosome 7 at 109,454,992 bp
T to C, chromosome 8 at 56,540,973 bp
AGAGGAGGAGGAGGAGGAGGAGGAGGA to AGAGGAGGAGGAGGAGGAGGAGGA, chromosome 9 at 20,873,717 bp
C to T, chromosome 9 at 20,876,527 bp
A to T, chromosome 9 at 54,541,349 bp
T to C, chromosome 9 at 113,964,668 bp
A to G, chromosome 10 at 24,101,503 bp
A to G, chromosome 10 at 62,956,989 bp
A to G, chromosome 10 at 63,134,923 bp
G to T, chromosome 10 at 71,262,110 bp
T to C, chromosome 10 at 127,884,729 bp
T to A, chromosome 11 at 72,198,845 bp
A to G, chromosome 11 at 101,287,498 bp
A to T, chromosome 11 at 106,285,426 bp
A to T, chromosome 12 at 11,289,164 bp
G to A, chromosome 12 at 70,988,495 bp
C to T, chromosome 12 at 78,881,238 bp
T to C, chromosome 12 at 108,866,018 bp
T to C, chromosome 13 at 24,993,759 bp
A to G, chromosome 13 at 33,899,269 bp
A to G, chromosome 14 at 29,467,949 bp
G to A, chromosome 14 at 75,126,110 bp
T to A, chromosome 14 at 79,419,268 bp
T to C, chromosome 15 at 38,677,528 bp
T to C, chromosome 15 at 58,553,724 bp
T to C, chromosome 15 at 101,778,305 bp
T to C, chromosome 16 at 17,907,555 bp
A to G, chromosome 16 at 21,906,524 bp
T to C, chromosome 16 at 32,143,423 bp
G to A, chromosome 16 at 32,794,480 bp
A to T, chromosome 16 at 33,550,904 bp
A to T, chromosome 16 at 45,677,962 bp
A to G, chromosome 16 at 85,899,726 bp
A to T, chromosome 17 at 25,105,823 bp
A to T, chromosome 17 at 25,936,762 bp
G to C, chromosome 17 at 37,111,455 bp
A to T, chromosome 18 at 37,402,255 bp
T to C, chromosome 19 at 26,706,200 bp
T to A, chromosome 19 at 33,559,625 bp
A to G, chromosome 19 at 50,241,942 bp
C to T, chromosome X at 71,820,157 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Suggested Control Mice

Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R0669 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Request Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
038854-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Request Information Expand All