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Strain Name:
C57BL/6J-MtgxR0731Btlr/Mmmh
Stock Number:
038912-MU
Citation ID:
RRID:MMRRC_038912-MU
Other Names:
R0731 (G1), C57BL/6J-MtgxR0731Btlr
Major Collection:

Strain Information

Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: MAPKKKK5, GCKR, KHS, 4432415E19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Hlcs
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 110948
VEGA: 16
HGNC: HGNC:4976
Homologene: 37302
Gpr83
Name: G protein-coupled receptor 83
Synonyms: RP105, RP82, RP39, glucocorticoid-induced receptor, GIR, Gpr72, Gir
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14608
HGNC: HGNC:4523
Homologene: 7732
Cdh11
Name: cadherin 11
Synonyms: osteoblast-cadherin, OB-cadherin, Cad11
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12552
HGNC: HGNC:1750
Homologene: 1361
Ahdc1
Name: AT hook, DNA binding motif, containing 1
Synonyms: D030015G18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230793
Homologene: 17144
Chuk
Name: conserved helix-loop-helix ubiquitous kinase
Synonyms: Chuk1, IKK1, IKK[a], IKK-alpha, IkappaB kinase alpha, IKK 1, IKK alpha, IKKalpha, IKK-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12675
HGNC: HGNC:1974
Homologene: 979
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 67,009,910 bp
  • T to A, chromosome 1 at 74,351,541 bp
  • T to A, chromosome 1 at 87,407,727 bp
  • A to C, chromosome 1 at 134,389,975 bp
  • C to T, chromosome 1 at 134,994,010 bp
  • A to T, chromosome 1 at 172,172,509 bp
  • A to G, chromosome 2 at 14,985,706 bp
  • A to G, chromosome 2 at 25,338,155 bp
  • A to G, chromosome 2 at 111,379,293 bp
  • G to T, chromosome 2 at 127,226,145 bp
  • A to G, chromosome 2 at 156,024,070 bp
  • T to A, chromosome 3 at 117,829,671 bp
  • G to A, chromosome 3 at 134,855,000 bp
  • T to A, chromosome 4 at 133,062,951 bp
  • T to A, chromosome 4 at 147,756,232 bp
  • G to A, chromosome 5 at 87,420,452 bp
  • A to G, chromosome 5 at 129,657,264 bp
  • A to G, chromosome 5 at 137,735,298 bp
  • T to C, chromosome 6 at 41,304,367 bp
  • G to T, chromosome 6 at 72,788,269 bp
  • A to G, chromosome 6 at 83,183,089 bp
  • A to T, chromosome 6 at 115,614,651 bp
  • T to A, chromosome 6 at 129,585,568 bp
  • A to C, chromosome 6 at 129,908,796 bp
  • T to C, chromosome 6 at 130,022,705 bp
  • T to C, chromosome 7 at 108,124,734 bp
  • A to T, chromosome 7 at 108,881,533 bp
  • T to C, chromosome 7 at 110,876,900 bp
  • A to T, chromosome 7 at 119,768,024 bp
  • A to G, chromosome 8 at 58,535,984 bp
  • A to G, chromosome 8 at 70,023,612 bp
  • A to G, chromosome 8 at 70,781,321 bp
  • T to C, chromosome 8 at 83,297,847 bp
  • G to A, chromosome 8 at 95,105,733 bp
  • T to A, chromosome 8 at 102,668,019 bp
  • A to G, chromosome 9 at 14,868,644 bp
  • T to C, chromosome 9 at 36,735,512 bp
  • T to C, chromosome 9 at 39,461,532 bp
  • A to G, chromosome 9 at 52,068,218 bp
  • T to C, chromosome 9 at 57,751,126 bp
  • T to C, chromosome 9 at 61,925,032 bp
  • A to T, chromosome 9 at 106,969,856 bp
  • A to G, chromosome 9 at 111,365,488 bp
  • C to T, chromosome 10 at 13,506,027 bp
  • C to A, chromosome 10 at 41,579,143 bp
  • T to A, chromosome 10 at 56,745,543 bp
  • T to C, chromosome 10 at 62,616,249 bp
  • G to A, chromosome 10 at 63,011,844 bp
  • T to C, chromosome 10 at 63,056,811 bp
  • T to C, chromosome 10 at 100,396,777 bp
  • T to C, chromosome 10 at 100,586,203 bp
  • A to G, chromosome 10 at 115,410,632 bp
  • A to G, chromosome 11 at 67,202,533 bp
  • G to T, chromosome 11 at 106,312,433 bp
  • A to G, chromosome 11 at 120,346,949 bp
  • T to A, chromosome 12 at 69,874,264 bp
  • A to T, chromosome 13 at 3,361,329 bp
  • A to T, chromosome 13 at 24,680,644 bp
  • A to T, chromosome 14 at 16,240,893 bp
  • T to A, chromosome 14 at 45,246,349 bp
  • C to A, chromosome 14 at 79,451,884 bp
  • A to T, chromosome 15 at 28,311,143 bp
  • A to G, chromosome 15 at 77,099,279 bp
  • T to C, chromosome 15 at 85,901,597 bp
  • T to A, chromosome 16 at 94,131,852 bp
  • T to C, chromosome 17 at 20,113,854 bp
  • T to A, chromosome 17 at 25,878,017 bp
  • T to A, chromosome 18 at 31,961,825 bp
  • C to T, chromosome 18 at 42,571,840 bp
  • A to T, chromosome 18 at 65,305,390 bp
  • T to G, chromosome 19 at 36,997,495 bp
  • A to G, chromosome 19 at 44,103,766 bp
  • T to A, chromosome 19 at 44,975,726 bp
  • T to C, chromosome 19 at 45,029,305 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0731 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038912-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.