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Strain Name:
C57BL/6J-MtgxR0962Btlr/Mmmh
Stock Number:
039091-MU
Citation ID:
RRID:MMRRC_039091-MU
Other Names:
R0962 (G1), C57BL/6J-MtgxR0962Btlr
Major Collection:

Strain Information

Ptprcap
Name: protein tyrosine phosphatase receptor type C polypeptide-associated protein
Synonyms: LSM-1, CD-45-AP
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19265
HGNC: HGNC:9667
Homologene: 48358
Cep57
Name: centrosomal protein 57
Synonyms: 4931428M20Rik, 4921510P06Rik, Tsp57, 3110002L15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74360
Homologene: 8790
Rabgap1
Name: RAB GTPase activating protein 1
Synonyms: Gapcena
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227800
Homologene: 49301
Utp6
Name: UTP6 small subunit processome component
Synonyms: HCA66, 4732497O03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216987
Homologene: 41265
Tex9
Name: testis expressed gene 9
Synonyms: tsec-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21778
Homologene: 32072
Brca1
Name: breast cancer 1, early onset
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12189
HGNC: HGNC:1100
Homologene: 5276
Stag1
Name: STAG1 cohesin complex component
Synonyms: SA-1, Scc3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20842
Homologene: 21191
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 14,898,163 bp
  • T to C, chromosome 1 at 82,886,396 bp
  • C to A, chromosome 1 at 90,142,731 bp
  • A to T, chromosome 1 at 92,899,568 bp
  • T to C, chromosome 2 at 37,560,469 bp
  • A to T, chromosome 2 at 76,950,156 bp
  • A to G, chromosome 2 at 120,280,617 bp
  • T to A, chromosome 3 at 95,684,488 bp
  • G to A, chromosome 4 at 98,945,195 bp
  • A to G, chromosome 4 at 100,983,301 bp
  • T to C, chromosome 4 at 147,882,201 bp
  • T to A, chromosome 5 at 35,668,356 bp
  • T to C, chromosome 5 at 98,566,561 bp
  • T to C, chromosome 5 at 114,123,953 bp
  • C to T, chromosome 5 at 142,161,875 bp
  • A to G, chromosome 6 at 39,147,194 bp
  • A to G, chromosome 7 at 45,347,722 bp
  • C to T, chromosome 7 at 101,384,914 bp
  • A to G, chromosome 7 at 103,122,010 bp
  • T to A, chromosome 7 at 107,148,087 bp
  • T to C, chromosome 7 at 112,380,417 bp
  • T to C, chromosome 7 at 137,225,203 bp
  • T to C, chromosome 8 at 13,001,964 bp
  • T to C, chromosome 8 at 45,033,326 bp
  • A to T, chromosome 9 at 13,808,743 bp
  • T to A, chromosome 9 at 20,137,538 bp
  • A to G, chromosome 9 at 35,000,997 bp
  • A to T, chromosome 9 at 54,446,412 bp
  • T to C, chromosome 9 at 72,484,092 bp
  • C to T, chromosome 9 at 80,381,741 bp
  • T to G, chromosome 9 at 95,670,034 bp
  • T to A, chromosome 9 at 100,796,827 bp
  • T to A, chromosome 10 at 7,590,296 bp
  • T to C, chromosome 10 at 76,292,432 bp
  • C to G, chromosome 10 at 127,742,306 bp
  • G to A, chromosome 11 at 70,975,041 bp
  • A to G, chromosome 11 at 79,941,868 bp
  • G to A, chromosome 11 at 84,311,303 bp
  • A to T, chromosome 11 at 101,525,366 bp
  • A to T, chromosome 11 at 118,393,020 bp
  • A to G, chromosome 12 at 7,989,191 bp
  • A to G, chromosome 12 at 108,327,721 bp
  • T to C, chromosome 13 at 81,405,346 bp
  • C to A, chromosome 13 at 100,179,385 bp
  • A to G, chromosome 13 at 105,444,324 bp
  • G to A, chromosome 14 at 56,368,413 bp
  • A to T, chromosome 14 at 64,896,774 bp
  • T to C, chromosome 16 at 13,130,146 bp
  • T to C, chromosome 16 at 25,997,248 bp
  • T to C, chromosome 18 at 67,405,427 bp
  • A to G, chromosome 19 at 4,156,464 bp
  • A to G, chromosome 19 at 9,012,848 bp
  • C to T, chromosome 19 at 11,471,142 bp
  • C to T, chromosome 19 at 55,210,284 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0962 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039091-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.