Strain Name:
C57BL/6J-MtgxR1004Btlr/Mmmh
Stock Number:
039114-MU
Citation ID:
RRID:MMRRC_039114-MU
Other Names:
R1004 (G1), C57BL/6J-MtgxR1004Btlr
Major Collection:

Gene Information

Ece1
Name: endothelin converting enzyme 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230857
HGNC: HGNC:3146
Homologene: 1068
Kdm5b
Name: lysine (K)-specific demethylase 5B
Synonyms: PLU-1, 2210016I17Rik, 2010009J12Rik, D1Ertd202e, Rb-Bp2, Plu1, Jarid1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75605
Homologene: 48448
Poli
Name: polymerase (DNA directed), iota
Synonyms: Rad30b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 26447
HGNC: HGNC:9182
Homologene: 5209
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: C130061D10Rik, Phr1, Pam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105689
Homologene: 9005
Arid1a
Name: AT rich interactive domain 1A (SWI-like)
Synonyms: BAF250a, 1110030E03Rik, Smarcf1, Osa1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 93760
Homologene: 21216
Xrcc5
Name: X-ray repair complementing defective repair in Chinese hamster cells 5
Synonyms: Ku86, Ku80
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22596
VEGA: 1
Homologene: 40681
Akap13
Name: A kinase (PRKA) anchor protein 13
Synonyms: PROTO-LBC, 1700026G02Rik, PROTO-LB, 5830460E08Rik, 5730522G15Rik, Ht31, AKAP-Lbc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Skp1
Name: S-phase kinase-associated protein 1
Synonyms: Skp1a, 2610206H23Rik, p19Skp1, Tceb1l, 2610043E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21402
Homologene: 38775
Gapvd1
Name: GTPase activating protein and VPS9 domains 1
Synonyms: 2010005B09Rik, 4432404J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66691
Homologene: 32637
Dlx6
Name: distal-less homeobox 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 13396
HGNC: HGNC:2919
Homologene: 87855
Hook1
Name: hook microtubule tethering protein 1
Synonyms: A930033L17Rik, azh, abnormal spermatozoon head shape
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 77963
Homologene: 9289
Ric1
Name: RAB6A GEF complex partner 1
Synonyms: C130057E09Rik, C030046E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226089
Homologene: 13806
Zfp600
Name: zinc finger protein 600
Synonyms: Gm13164
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 667666
Homologene: 133076
Nxf1
Name: nuclear RNA export factor 1
Synonyms: TAP, Mvb1, Tip associated protein, Mex67
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 53319
HGNC: HGNC:8071
Homologene: 38176
Nupl1
Name: nucleoporin like 1
Synonyms: 1700017F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71844
VEGA: 14
Homologene: 40924
Slc12a9
Name: solute carrier family 12 (potassium/chloride transporters), member 9
Synonyms: CIP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 83704
Homologene: 5429
Sh3bgrl2
Name: SH3 domain binding glutamic acid-rich protein like 2
Synonyms: A930014C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 212531
Homologene: 12876
Anpep
Name: alanyl (membrane) aminopeptidase
Synonyms: Cd13, aminopeptidase N, Apn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16790
HGNC: HGNC:500
Homologene: 68163
Gm872
Name: predicted gene 872
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Ptchd4
Name: patched domain containing 4
Synonyms: 3110082D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627626
VEGA: 17
Homologene: 78322
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: Heatr7b1, ENSMUSG00000044873, OTTMUSG00000020804
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Agbl3
Name: ATP/GTP binding protein-like 3
Synonyms: Ccp3, 2900053G10Rik, 6530406M24Rik, Ccp3, 4930431N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76223
Homologene: 35330
Gatm
Name: glycine amidinotransferase (L-arginine:glycine amidinotransferase)
Synonyms: 1810003P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67092
HGNC: HGNC:4175
Homologene: 1136
Abca9
Name: ATP-binding cassette, sub-family A (ABC1), member 9
Synonyms: D630040K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217262
HGNC: HGNC:39
Homologene: 33332
Zfp235
Name: zinc finger protein 235
Synonyms: 0610030O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56525
Homologene: 122146
Col11a1
Name: collagen, type XI, alpha 1
Synonyms: C530001D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12814
HGNC: HGNC:2186
Homologene: 56389
Serpinb9f
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9f
Synonyms: Spi13, NK21, ovalbumin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20709
HGNC: HGNC:8955
Homologene: 69093
Oaz3
Name: ornithine decarboxylase antizyme 3
Synonyms: AZ-3, Oaz-t, AZ3, ornithine decarboxylase antizyme in testis, antizyme 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 53814
HGNC: HGNC:8097
Homologene: 9410
Gabbr2
Name: gamma-aminobutyric acid (GABA) B receptor, 2
Synonyms: Gababr2, Gpr51, GB2, LOC242425
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242425
HGNC: HGNC:4507
Homologene: 55902
Ppp2r3a
Name: protein phosphatase 2, regulatory subunit B'', alpha
Synonyms: 3222402P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235542
HGNC: HGNC:9307
Homologene: 20595
Pfpl
Name: pore forming protein-like
Synonyms: Epcs5, Epcs50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56093
VEGA: 19
Homologene: 130704
Mettl9
Name: methyltransferase like 9
Synonyms: Drev, 0610012D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 59052
Homologene: 41092
Cd163
Name: CD163 antigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 93671
HGNC: HGNC:1631
Homologene: 128811
Dpp4
Name: dipeptidylpeptidase 4
Synonyms: Cd26, Dpp-4, THAM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13482
HGNC: HGNC:3009
Homologene: 3279
Ces2e
Name: carboxylesterase 2E
Synonyms: 9030624L02Rik, Ces5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234673
HGNC: HGNC:1864
Homologene: 86210
Gpc2
Name: glypican 2 (cerebroglycan)
Synonyms: 2410016G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71951
HGNC: HGNC:4450
Homologene: 17657
Olfr971
Name: olfactory receptor 971
Synonyms: MOR171-13, GA_x6K02T2PVTD-33539896-33540819
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258607
VEGA: 9
Homologene: 133635
Agxt
Name: alanine-glyoxylate aminotransferase
Synonyms: Agxt1, Agt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11611
HGNC: HGNC:341
Homologene: 37251
AC238940.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Prr30
Name: proline rich 30
Synonyms: 1700110M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 76627
Homologene: 130773
Slc22a6
Name: solute carrier family 22 (organic anion transporter), member 6
Synonyms: Orctl1, Oat1, NKT, mOat1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18399
VEGA: 19
Homologene: 16813
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 72,383,778 bp
  • C to A, chromosome 1 at 88,242,420 bp
  • A to G, chromosome 1 at 93,135,699 bp
  • T to A, chromosome 1 at 134,588,904 bp
  • A to G, chromosome 2 at 34,706,735 bp
  • T to A, chromosome 2 at 62,332,640 bp
  • C to T, chromosome 2 at 122,609,660 bp
  • T to A, chromosome 3 at 94,435,043 bp
  • A to G, chromosome 3 at 114,095,022 bp
  • C to T, chromosome 4 at 46,677,544 bp
  • A to C, chromosome 4 at 96,022,287 bp
  • A to G, chromosome 4 at 133,687,275 bp
  • A to G, chromosome 4 at 137,926,239 bp
  • T to A, chromosome 4 at 146,196,533 bp
  • T to C, chromosome 5 at 137,322,524 bp
  • A to G, chromosome 5 at 138,278,225 bp
  • C to T, chromosome 6 at 6,863,665 bp
  • A to T, chromosome 6 at 34,803,451 bp
  • G to T, chromosome 6 at 124,325,347 bp
  • T to A, chromosome 7 at 24,140,744 bp
  • T to C, chromosome 7 at 75,687,286 bp
  • C to T, chromosome 7 at 79,838,256 bp
  • G to A, chromosome 7 at 121,076,237 bp
  • A to G, chromosome 8 at 104,929,738 bp
  • T to C, chromosome 9 at 39,839,980 bp
  • C to T, chromosome 9 at 83,577,631 bp
  • C to T, chromosome 9 at 101,198,630 bp
  • T to C, chromosome 10 at 93,066,696 bp
  • G to C, chromosome 11 at 52,237,380 bp
  • A to G, chromosome 11 at 110,151,954 bp
  • TA to TTTNA,T, chromosome 13 at 33,334,242 bp
  • T to A, chromosome 13 at 120,155,022 bp
  • C to A, chromosome 14 at 60,247,481 bp
  • A to G, chromosome 14 at 101,199,093 bp
  • G to A, chromosome 14 at 103,140,917 bp
  • A to T, chromosome 17 at 42,377,602 bp
  • T to A, chromosome 18 at 70,525,438 bp
  • A to G, chromosome 19 at 8,618,399 bp
  • A to T, chromosome 19 at 8,764,317 bp
  • A to T, chromosome 19 at 12,430,425 bp
  • A to G, chromosome 19 at 29,602,357 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1004 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039114-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.