Strain Name:
C57BL/6J-MtgxR1025Btlr/Mmmh
Stock Number:
039127-MU
Citation ID:
RRID:MMRRC_039127-MU
Other Names:
R1025 (G1), C57BL/6J-MtgxR1025Btlr
Major Collection:

Gene Information

Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Pkn2
Name: protein kinase N2
Synonyms: PRK2, 6030436C20Rik, Stk7, Prkcl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 109333
HGNC: HGNC:9406
Homologene: 2054
Ap1g1
Name: adaptor protein complex AP-1, gamma 1 subunit
Synonyms: gamma-adaptin, Adtg, D8Ertd374e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11765
HGNC: HGNC:555
Homologene: 47995
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: 5830451P18Rik, Duplin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67772
Homologene: 72405
Pdcd6ip
Name: programmed cell death 6 interacting protein
Synonyms: Alix, AIP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18571
HGNC: HGNC:8766
Homologene: 22614
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Baz2b
Name: bromodomain adjacent to zinc finger domain, 2B
Synonyms: D2Ertd794e, 5830435C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 407823
HGNC: HGNC:963
Homologene: 8394
Tarsl2
Name: threonyl-tRNA synthetase-like 2
Synonyms: A530046H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 272396
Homologene: 65036
Trpa1
Name: transient receptor potential cation channel, subfamily A, member 1
Synonyms: ANKTM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 277328
HGNC: HGNC:497
Homologene: 7189
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: 4833413O10Rik, D430029O09Rik, 1110021D17Rik, Polydom
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 64817
Homologene: 23386
Gabra2
Name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2
Synonyms: Gabra-2, C630048P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14395
HGNC: HGNC:4076
Homologene: 20217
Vmn2r57
Name: vomeronasal 2, receptor 57
Synonyms: EG269902
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269902
Homologene: 104832
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241516
Homologene: 110349
Irak3
Name: interleukin-1 receptor-associated kinase 3
Synonyms: IRAK-M, 4833428C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 73914
Homologene: 36215
3110009E18Rik
Name: RIKEN cDNA 3110009E18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 73103
Homologene: 41754
Zfp811
Name: zinc finger protein 811
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240063
Olfr307
Name: olfactory receptor 307
Synonyms: GA_x6K02T2NHDJ-9772012-9772953, MOR219-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258610
Acte1
Name: actin, epsilon 1
Synonyms: LOC244239, Gm498
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 102636989
Homologene: 131918
Slc26a4
Name: solute carrier family 26, member 4
Synonyms: Pds, pendrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 23985
VEGA: 12
HGNC: HGNC:8818
Homologene: 20132
Alyref
Name: Aly/REF export factor
Synonyms: REF1, Refbp1, Thoc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21681
Homologene: 38099
Atp8a2
Name: ATPase, aminophospholipid transporter-like, class I, type 8A, member 2
Synonyms: Ib, wl, agil
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50769
Homologene: 4443
Vmn2r17
Name: vomeronasal 2, receptor 17
Synonyms: EG384221
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384221
Homologene: 104825
1700067P10Rik
Name: RIKEN cDNA 1700067P10 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 68224
VEGA: 17
Homologene: 87035
Cpne4
Name: copine IV
Synonyms: 3632411M23Rik, 4933406O10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74020
HGNC: HGNC:2317
Homologene: 26078
Vmn1r17
Name: vomeronasal 1 receptor 17
Synonyms: V1rc16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171189
Homologene: 115643
Olfr1415
Name: olfactory receptor 1415
Synonyms: GA_x6K02T2R7CC-81266841-81267776, MOR103-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258228
Homologene: 79367
Cers1
Name: ceramide synthase 1
Synonyms: Uog-1, to, CerS1, Lass1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 93898
Homologene: 128762
Cebpzos
Name: CCAAT/enhancer binding protein (C/EBP), zeta, opposite strand
Synonyms: 1110001A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 68554
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 14,904,183 bp
  • T to A, chromosome 1 at 92,491,723 bp
  • C to T, chromosome 1 at 120,171,462 bp
  • T to C, chromosome 2 at 59,962,482 bp
  • C to A, chromosome 2 at 82,989,436 bp
  • A to G, chromosome 3 at 142,821,565 bp
  • C to T, chromosome 4 at 58,087,817 bp
  • T to C, chromosome 4 at 123,473,816 bp
  • T to C, chromosome 5 at 70,973,595 bp
  • A to G, chromosome 5 at 109,452,475 bp
  • A to T, chromosome 6 at 57,361,255 bp
  • A to G, chromosome 7 at 41,427,804 bp
  • G to A, chromosome 7 at 65,663,864 bp
  • T to C, chromosome 7 at 86,335,905 bp
  • A to G, chromosome 7 at 143,896,390 bp
  • A to T, chromosome 8 at 70,321,536 bp
  • G to A, chromosome 8 at 109,818,939 bp
  • A to T, chromosome 9 at 104,993,858 bp
  • G to A, chromosome 9 at 113,662,286 bp
  • A to T, chromosome 10 at 120,176,346 bp
  • C to G, chromosome 11 at 120,595,932 bp
  • T to G, chromosome 12 at 31,528,737 bp
  • C to T, chromosome 14 at 52,216,456 bp
  • T to C, chromosome 14 at 59,860,270 bp
  • A to G, chromosome 17 at 32,798,644 bp
  • A to T, chromosome 17 at 48,090,510 bp
  • A to G, chromosome 17 at 67,752,898 bp
  • A to G, chromosome 17 at 78,918,388 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1025 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039127-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.