Strain Name:
C57BL/6J-MtgxR1164Btlr/Mmmh
Stock Number:
039237-MU
Citation ID:
RRID:MMRRC_039237-MU
Other Names:
R1164 (G1), C57BL/6J-MtgxR1164Btlr
Major Collection:

Gene Information

Gene Symbol: Sema3c [MGI:107557] (Mus musculus (mouse))
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Ptprf [MGI:102695] (Mus musculus (mouse))
Name: protein tyrosine phosphatase, receptor type, F
Synonyms: RPTP-LAR, LAR
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Cks1b [MGI:1889208] (Mus musculus (mouse))
Name: CDC28 protein kinase 1b
Synonyms: 2410005G18Rik, 2610005D03Rik, Cks1
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Scn8a [MGI:103169] (Mus musculus (mouse))
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: nmf335, med, nur14, seal, Nav1.6, ataxia 3, NaCh6, nmf58, C630029C19Rik, NMF335, mnd-2, nmf2, mnd2, motor end-plate disease
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Utp4 [MGI:1096573] (Mus musculus (mouse))
Name: UTP4 small subunit processome component
Synonyms: TEG-292, Tex292, Cirh1a
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: 9630014M24Rik [MGI:3588234] (Mus musculus (mouse))
Name: RIKEN cDNA 9630014M24 gene
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Rere [MGI:2683486] (Mus musculus (mouse))
Name: arginine glutamic acid dipeptide (RE) repeats
Synonyms: eyes3, 1110033A15Rik, eyem03Jus, atrophin-2, Atr2
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 46,029,039 bp
  • A to T, chromosome 1 at 87,192,545 bp
  • G to A, chromosome 1 at 133,659,203 bp
  • T to C, chromosome 1 at 135,472,410 bp
  • A to T, chromosome 1 at 171,029,170 bp
  • A to G, chromosome 1 at 171,158,439 bp
  • A to G, chromosome 1 at 175,658,559 bp
  • T to C, chromosome 2 at 5,141,266 bp
  • A to G, chromosome 2 at 25,574,715 bp
  • A to T, chromosome 2 at 28,962,688 bp
  • A to T, chromosome 2 at 36,640,120 bp
  • A to G, chromosome 2 at 58,763,704 bp
  • C to A, chromosome 2 at 86,508,684 bp
  • T to C, chromosome 2 at 158,783,250 bp
  • A to G, chromosome 3 at 34,650,699 bp
  • T to C, chromosome 3 at 58,676,316 bp
  • C to A, chromosome 3 at 89,415,942 bp
  • A to G, chromosome 3 at 141,211,499 bp
  • T to C, chromosome 4 at 43,770,991 bp
  • A to C, chromosome 4 at 68,798,691 bp
  • A to G, chromosome 4 at 107,887,262 bp
  • T to A, chromosome 4 at 115,918,258 bp
  • G to A, chromosome 4 at 117,876,944 bp
  • A to G, chromosome 4 at 118,257,492 bp
  • A to G, chromosome 4 at 118,965,405 bp
  • T to C, chromosome 4 at 132,732,411 bp
  • A to T, chromosome 4 at 140,832,329 bp
  • A to T, chromosome 4 at 150,534,884 bp
  • G to A, chromosome 5 at 16,361,876 bp
  • G to A, chromosome 5 at 17,678,314 bp
  • A to G, chromosome 5 at 27,721,105 bp
  • T to A, chromosome 5 at 62,683,477 bp
  • G to A, chromosome 5 at 103,489,773 bp
  • A to T, chromosome 5 at 136,130,990 bp
  • T to A, chromosome 6 at 29,754,719 bp
  • T to A, chromosome 7 at 5,454,411 bp
  • T to C, chromosome 7 at 12,971,624 bp
  • T to A, chromosome 7 at 30,717,692 bp
  • A to T, chromosome 7 at 105,180,833 bp
  • G to T, chromosome 7 at 118,542,024 bp
  • G to A, chromosome 8 at 80,710,631 bp
  • A to G, chromosome 8 at 106,900,844 bp
  • C to T, chromosome 8 at 111,288,242 bp
  • C to T, chromosome 8 at 123,421,447 bp
  • C to T, chromosome 10 at 18,783,721 bp
  • T to C, chromosome 10 at 41,464,527 bp
  • C to A, chromosome 10 at 76,276,397 bp
  • T to C, chromosome 11 at 59,036,087 bp
  • T to C, chromosome 12 at 17,308,316 bp
  • A to G, chromosome 12 at 102,563,045 bp
  • A to G, chromosome 14 at 28,302,972 bp
  • T to C, chromosome 14 at 50,792,137 bp
  • A to G, chromosome 15 at 75,902,677 bp
  • G to A, chromosome 15 at 88,820,631 bp
  • G to A, chromosome 15 at 101,040,162 bp
  • A to G, chromosome 16 at 17,558,159 bp
  • G to A, chromosome 16 at 31,894,682 bp
  • A to T, chromosome 16 at 38,450,340 bp
  • A to G, chromosome 16 at 57,049,426 bp
  • T to C, chromosome 17 at 8,439,939 bp
  • G to A, chromosome 17 at 21,879,979 bp
  • T to A, chromosome 17 at 24,402,331 bp
  • T to G, chromosome 17 at 24,722,783 bp
  • T to C, chromosome 17 at 27,895,380 bp
  • T to A, chromosome 17 at 37,850,684 bp
  • A to C, chromosome 17 at 55,674,085 bp
  • A to G, chromosome 17 at 69,274,767 bp
  • A to C, chromosome 18 at 38,642,375 bp
  • A to T, chromosome 19 at 12,074,241 bp
  • A to T, chromosome 19 at 20,617,946 bp
  • A to G, chromosome 19 at 25,090,027 bp
  • A to G, chromosome 19 at 25,677,993 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1164 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039237-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.