Strain Name:
C57BL/6J-MtgxR1214Btlr/Mmmh
Stock Number:
039283-MU
Citation ID:
RRID:MMRRC_039283-MU
Other Names:
R1214 (G1), C57BL/6J-MtgxR1214Btlr
Major Collection:

Gene Information

Slc26a5
Name: solute carrier family 26, member 5
Synonyms: Pres, prestin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Dock9
Name: dedicator of cytokinesis 9
Synonyms: B230309H04Rik, D14Wsu89e, Zizimin1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105445
Homologene: 41026
Tut7
Name: terminal uridylyl transferase 7
Synonyms: 6030448M23Rik, Tent3b, Zcchc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 214290
VEGA: 13
Homologene: 51941
Dync1h1
Name: dynein cytoplasmic 1 heavy chain 1
Synonyms: MAP1C, dynein heavy chain, retrograde transport, Dnec1, Loa, 9930018I23Rik, Dnchc1, Swl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13424
HGNC: HGNC:2961
Homologene: 1053
Fam160a2
Name: family with sequence similarity 160, member A2
Synonyms: 4632419K20Rik, 6530415H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74349
Homologene: 75329
Msh3
Name: mutS homolog 3
Synonyms: D13Em1, Rep-3, Rep3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17686
HGNC: HGNC:7326
Homologene: 1829
Wasf3
Name: WASP family, member 3
Synonyms: Wave3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 245880
Homologene: 68527
Usp2
Name: ubiquitin specific peptidase 2
Synonyms: ubp41, B930035K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 53376
Homologene: 3098
Olfr1368
Name: olfactory receptor 1368
Synonyms: GA_x6K02T2QHY8-12104556-12105500, MOR256-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 258527
Homologene: 105156
Ptpre
Name: protein tyrosine phosphatase, receptor type, E
Synonyms: PTPe, PTPepsilon, RPTPepsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19267
HGNC: HGNC:9669
Homologene: 31387
Alkbh2
Name: alkB homolog 2, alpha-ketoglutarate-dependent dioxygenase
Synonyms: Abh2, mABH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231642
Homologene: 18393
Ttc29
Name: tetratricopeptide repeat domain 29
Synonyms: 1700031F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 73301
Homologene: 12918
Slfn5
Name: schlafen 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327978
Homologene: 18839
Adamtsl2
Name: ADAMTS-like 2
Synonyms: A930008K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 77794
Homologene: 8798
Olfr341
Name: olfactory receptor 341
Synonyms: GA_x6K02T2NLDC-33174915-33173974, MOR136-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258952
Homologene: 74160
Olfr1289
Name: olfactory receptor 1289
Synonyms: GA_x6K02T2Q125-72534883-72535821, MOR245-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258399
HGNC: HGNC:8301
Micall2
Name: MICAL-like 2
Synonyms: A930021H16Rik, Jrab, MICAL-L2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231830
Homologene: 85155
Dcdc2c
Name: doublecortin domain containing 2C
Synonyms: 1110015M06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68511
Homologene: 131934
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 2 at 27,102,985 bp
  • G to T, chromosome 2 at 36,479,969 bp
  • T to C, chromosome 2 at 111,483,892 bp
  • T to A, chromosome 5 at 21,814,983 bp
  • C to T, chromosome 5 at 114,124,226 bp
  • T to C, chromosome 5 at 139,711,641 bp
  • G to A, chromosome 5 at 146,470,288 bp
  • A to G, chromosome 7 at 105,381,386 bp
  • A to T, chromosome 7 at 135,679,258 bp
  • A to G, chromosome 8 at 78,325,582 bp
  • C to T, chromosome 9 at 44,089,123 bp
  • A to C, chromosome 10 at 76,719,177 bp
  • A to G, chromosome 11 at 82,960,091 bp
  • A to T, chromosome 12 at 28,530,430 bp
  • G to A, chromosome 12 at 110,636,509 bp
  • C to T, chromosome 13 at 21,142,167 bp
  • A to G, chromosome 13 at 59,805,326 bp
  • A to G, chromosome 13 at 92,285,862 bp
  • C to T, chromosome 14 at 121,586,316 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1214 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039283-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.