Strain Name:
C57BL/6J-MtgxR1228Btlr/Mmmh
Stock Number:
039297-MU
Citation ID:
RRID:MMRRC_039297-MU
Other Names:
R1228 (G1), C57BL/6J-MtgxR1228Btlr
Major Collection:

Gene Information

Sh3gl3
Name: SH3-domain GRB2-like 3
Synonyms: SH3P13, Sh3d2c, Sh3d2c2, EEN-B2, endophilin III
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20408
Homologene: 37723
Mast4
Name: microtubule associated serine/threonine kinase family member 4
Synonyms: 4930420O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 328329
Homologene: 42094
Gtf3c3
Name: general transcription factor IIIC, polypeptide 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98488
HGNC: HGNC:4666
Homologene: 40805
Plxna4
Name: plexin A4
Synonyms: Plxa4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243743
HGNC: HGNC:9102
Homologene: 77587
Enpp1
Name: ectonucleotide pyrophosphatase/phosphodiesterase 1
Synonyms: Pdnp1, 4833416E15Rik, Pca-1, NPP1, Pca, E-NPP1, Ly-41, PC-1, twy, CD203c, Npps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18605
HGNC: HGNC:3356
Homologene: 38151
Akap2
Name: A kinase (PRKA) anchor protein 2
Synonyms: B230340M18Rik, AKAP-KL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Clvs2
Name: clavesin 2
Synonyms: Rlbp1l2, A330019N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215890
Homologene: 27894
Fli1
Name: Friend leukemia integration 1
Synonyms: SIC-1, EWSR2, Sic1, Fli-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 14247
HGNC: HGNC:3749
Homologene: 55624
Ccdc181
Name: coiled-coil domain containing 181
Synonyms: 4930455F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74895
Homologene: 10912
Tcea2
Name: transcription elongation factor A (SII), 2
Synonyms: SII-T1, Tceat, S-II-T1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21400
Homologene: 68304
Stat4
Name: signal transducer and activator of transcription 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20849
Homologene: 20679
Capn7
Name: calpain 7
Synonyms: PalBH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12339
HGNC: HGNC:1484
Homologene: 7651
Rims1
Name: regulating synaptic membrane exocytosis 1
Synonyms: RIM1a, RIM1alpha, C030033M19Rik, RIM1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 116837
Homologene: 128399
Vmn2r77
Name: vomeronasal 2, receptor 77
Synonyms: EG546983
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 546983
Homologene: 115466
Skint6
Name: selection and upkeep of intraepithelial T cells 6
Synonyms: OTTMUSG00000008519
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230622
Homologene: 135888
Pappa
Name: pregnancy-associated plasma protein A
Synonyms: PAPP-A, PAG1, IGFBP-4ase, 8430414N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18491
HGNC: HGNC:8602
Homologene: 31097
Krt13
Name: keratin 13
Synonyms: K13, Krt1-13, Krt-1.13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16663
HGNC: HGNC:6415
Homologene: 40740
Plch2
Name: phospholipase C, eta 2
Synonyms: PLCeta2, A930027K05Rik, Plcl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269615
Homologene: 85172
Ttbk1
Name: tau tubulin kinase 1
Synonyms: C330008L01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106763
VEGA: 17
Homologene: 50342
Klhl25
Name: kelch-like 25
Synonyms: 2810402K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 207952
Homologene: 32546
Bag6
Name: BCL2-associated athanogene 6
Synonyms: G3, D17H6S52E, Scythe, Bat3, 2410045D21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224727
Homologene: 3409
Spata7
Name: spermatogenesis associated 7
Synonyms: B230306G18Rik, HSD3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104871
Homologene: 10189
Sned1
Name: sushi, nidogen and EGF-like domains 1
Synonyms: 6720455I24Rik, Snep, D430044C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208777
Homologene: 14708
Fam189a2
Name: family with sequence similarity 189, member A2
Synonyms: LOC381217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 381217
VEGA: 19
Homologene: 3540
Rabac1
Name: Rab acceptor 1 (prenylated)
Synonyms: Gbpap1, 2310040I06Rik, PRA1, prenylin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14470
HGNC: HGNC:9794
Homologene: 38182
Sp110
Name: Sp110 nuclear body protein
Synonyms: 5031415C07Rik, Ipr1, 5830484A20Rik, 52kDa, Ifi75
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 109032
HGNC: HGNC:5401
Homologene: 82192
Phc3
Name: polyhomeotic 3
Synonyms: EDR3, HPH3, E030046K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 241915
Homologene: 69390
Rpia
Name: ribose 5-phosphate isomerase A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19895
Homologene: 6943
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 22,472,756 bp
  • A to G, chromosome 1 at 52,076,693 bp
  • C to T, chromosome 1 at 54,417,778 bp
  • G to A, chromosome 1 at 85,591,760 bp
  • G to A, chromosome 1 at 93,281,654 bp
  • C to T, chromosome 1 at 164,286,391 bp
  • G to T, chromosome 2 at 181,684,445 bp
  • A to T, chromosome 3 at 30,922,255 bp
  • T to A, chromosome 4 at 57,856,909 bp
  • T to C, chromosome 4 at 65,340,689 bp
  • T to C, chromosome 4 at 112,854,452 bp
  • A to G, chromosome 4 at 154,993,035 bp
  • A to T, chromosome 6 at 32,224,152 bp
  • T to C, chromosome 6 at 70,791,896 bp
  • T to C, chromosome 7 at 24,972,098 bp
  • C to T, chromosome 7 at 75,866,120 bp
  • A to G, chromosome 7 at 82,174,975 bp
  • G to A, chromosome 7 at 86,801,034 bp
  • T to C, chromosome 9 at 32,423,843 bp
  • T to A, chromosome 10 at 24,645,412 bp
  • T to C, chromosome 10 at 33,622,604 bp
  • T to C, chromosome 11 at 100,121,477 bp
  • T to C, chromosome 12 at 98,634,269 bp
  • T to A, chromosome 13 at 102,804,724 bp
  • A to G, chromosome 14 at 31,358,600 bp
  • T to A, chromosome 17 at 35,145,333 bp
  • A to T, chromosome 17 at 46,476,712 bp
  • A to G, chromosome 19 at 23,979,465 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1228 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039297-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.