Strain Name:
C57BL/6J-MtgxR1235Btlr/Mmmh
Stock Number:
039303-MU
Citation ID:
RRID:MMRRC_039303-MU
Other Names:
R1235 (G1), C57BL/6J-MtgxR1235Btlr
Major Collection:

Gene Information

Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Plcb4
Name: phospholipase C, beta 4
Synonyms: C230058B11Rik, A930039J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18798
HGNC: HGNC:9059
Homologene: 8471
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 73884
Homologene: 52868
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Mtbp
Name: Mdm2, transformed 3T3 cell double minute p53 binding protein
Synonyms: MDM2BP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105837
HGNC: HGNC:7417
Homologene: 11102
Arsg
Name: arylsulfatase G
Synonyms: 6330406P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74008
Homologene: 8978
Cyp20a1
Name: cytochrome P450, family 20, subfamily a, polypeptide 1
Synonyms: A930011N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77951
Homologene: 18584
Zp2
Name: zona pellucida glycoprotein 2
Synonyms: Zp-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22787
Homologene: 48194
Trpc7
Name: transient receptor potential cation channel, subfamily C, member 7
Synonyms: TRP7, Trrp8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 26946
Homologene: 22689
Pld1
Name: phospholipase D1
Synonyms: Pld1b, Pld1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18805
HGNC: HGNC:9067
Homologene: 116234
Pcdhb13
Name: protocadherin beta 13
Synonyms: PcdhbM, Pcdbh6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93884
HGNC: HGNC:8691
Homologene: 10338
Sema5a
Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Synonyms: M-Sema D, semF, Semaf, 9130201M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20356
VEGA: 15
Homologene: 2949
Gys2
Name: glycogen synthase 2
Synonyms: LGS, glycogen synthase, liver
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232493
HGNC: HGNC:4707
Homologene: 56580
Myom3
Name: myomesin family, member 3
Synonyms: 8430427K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242702
Homologene: 19432
Nmur1
Name: neuromedin U receptor 1
Synonyms: FM-3, NmU-R, NMU1R, Gpr66
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14767
HGNC: HGNC:4518
Homologene: 68501
Vmn2r106
Name: vomeronasal 2, receptor 106
Synonyms: EG224576
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224576
Homologene: 135824
Kcnh3
Name: potassium voltage-gated channel, subfamily H (eag-related), member 3
Synonyms: ether a go-go like, Elk2, Melk2, C030044P22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16512
VEGA: 15
HGNC: HGNC:6252
Homologene: 32150
Rem1
Name: rad and gem related GTP binding protein 1
Synonyms: E030011C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19700
Homologene: 7514
Dnajb3
Name: DnaJ heat shock protein family (Hsp40) member B3
Synonyms: MSJ-1, Hsj3, Msj1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 15504
Homologene: 56409
Olfr1132
Name: olfactory receptor 1132
Synonyms: GA_x6K02T2Q125-49140947-49140021, MOR177-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258833
Homologene: 121518
Fam53c
Name: family with sequence similarity 53, member C
Synonyms: 2810012G03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 66306
VEGA: 18
HGNC: HGNC:1336
Homologene: 9586
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 60,363,364 bp
  • A to G, chromosome 1 at 63,309,073 bp
  • C to T, chromosome 1 at 86,386,693 bp
  • C to T, chromosome 1 at 88,205,479 bp
  • C to T, chromosome 2 at 69,524,036 bp
  • T to G, chromosome 2 at 87,634,815 bp
  • G to A, chromosome 2 at 135,972,948 bp
  • G to A, chromosome 2 at 152,634,535 bp
  • A to T, chromosome 3 at 28,028,734 bp
  • A to T, chromosome 4 at 135,789,543 bp
  • A to T, chromosome 6 at 142,430,293 bp
  • A to T, chromosome 7 at 120,138,343 bp
  • C to A, chromosome 10 at 76,712,324 bp
  • T to C, chromosome 11 at 109,534,107 bp
  • G to T, chromosome 13 at 56,887,539 bp
  • A to T, chromosome 15 at 32,609,226 bp
  • A to G, chromosome 15 at 55,567,174 bp
  • T to A, chromosome 15 at 99,242,103 bp
  • A to T, chromosome 17 at 20,279,479 bp
  • T to A, chromosome 18 at 34,768,258 bp
  • T to C, chromosome 18 at 37,444,959 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1235 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039303-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.