Strain Name:
C57BL/6J-MtgxR1246Btlr/Mmmh
Stock Number:
039313-MU
Citation ID:
RRID:MMRRC_039313-MU
Other Names:
R1246 (G1), C57BL/6J-MtgxR1246Btlr
Major Collection:

Strain Information

Pik3cd
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Synonyms: 2410099E07Rik, p110delta, 2610208K16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18707
HGNC: HGNC:8977
Homologene: 3686
Ythdf2
Name: YTH N6-methyladenosine RNA binding protein 2
Synonyms: 9430020E02Rik, NY-REN-2, HGRG8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 213541
Homologene: 32302
4833420G17Rik
Name: RIKEN cDNA 4833420G17 gene
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 67392
VEGA: 13
Homologene: 18889
Zzef1
Name: zinc finger, ZZ-type with EF hand domain 1
Synonyms: 8430405D05Rik, C130099L13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 195018
Homologene: 9027
Nol8
Name: nucleolar protein 8
Synonyms: D13Ertd548e, 5730412B09Rik, 4921532D18Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70930
VEGA: 13
Homologene: 41210
Pde4d
Name: phosphodiesterase 4D, cAMP specific
Synonyms: dunce, 9630011N22Rik, Dpde3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238871
HGNC: HGNC:8783
1700029F12Rik
Name: RIKEN cDNA 1700029F12 gene
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66479
VEGA: 13
Pigg
Name: phosphatidylinositol glycan anchor biosynthesis, class G
Synonyms: Gpi7
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 433931
Homologene: 39421
Ltbp1
Name: latent transforming growth factor beta binding protein 1
Synonyms: Ltbp1L, b2b1000Clo, 9430031G15Rik, LTBP-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 268977
HGNC: HGNC:6714
Homologene: 522
Gm7361
Name: predicted gene 7361
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 664837
Homologene: 69402
Rap1gap
Name: Rap1 GTPase-activating protein
Synonyms: Gap, 2310004O14Rik, Rap1ga1, 1300019I11Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 110351
HGNC: HGNC:9858
Homologene: 2163
Grik2
Name: glutamate receptor, ionotropic, kainate 2 (beta 2)
Synonyms: Glur6, C130030K03Rik, Glur-6, Glurbeta2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14806
HGNC: HGNC:4580
Homologene: 40717
Krtap4-25
Name: keratin associated protein 4-25
Synonyms: 2310045F10Rik, Gm11563
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 100040248
Homologene: 136093
Or56b2
Name: olfactory receptor family 56 subfamily B member 2
Synonyms: GA_x6K02T2PBJ9-7316375-7317334, Olfr661, MOR40-4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258743
Homologene: 17376
Or8b1
Name: olfactory receptor family 8 subfamily B member 1
Synonyms: Olfr906, MOR167-2, GA_x6K02T2PVTD-32194085-32195020
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258799
VEGA: 9
Homologene: 132439
Or52b4
Name: olfactory receptor family 52 subfamily B member 4
Synonyms: GA_x6K02T2PBJ9-5256044-5256988, MOR31-4, Olfr547
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259083
Homologene: 17493
Tubb2b
Name: tubulin, beta 2B class IIB
Synonyms: brdp, 2410129E14Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 73710
VEGA: 13
Homologene: 49734
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 4 at 132,204,871 bp
  • A to G, chromosome 4 at 137,712,094 bp
  • A to G, chromosome 4 at 149,659,800 bp
  • G to T, chromosome 5 at 26,261,227 bp
  • T to C, chromosome 5 at 108,341,820 bp
  • T to A, chromosome 7 at 102,534,942 bp
  • C to A, chromosome 7 at 104,688,164 bp
  • T to C, chromosome 9 at 38,488,790 bp
  • AGGG to AGG, chromosome 10 at 49,573,827 bp
  • A to G, chromosome 11 at 72,874,909 bp
  • T to C, chromosome 11 at 99,658,848 bp
  • G to A, chromosome 13 at 34,128,147 bp
  • T to A, chromosome 13 at 49,676,769 bp
  • T to C, chromosome 13 at 97,030,295 bp
  • T to A, chromosome 13 at 109,950,973 bp
  • T to A, chromosome 13 at 119,477,866 bp
  • T to A, chromosome 17 at 75,385,161 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1246 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039313-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.