Strain Name:
C57BL/6J-MtgxR1255Btlr/Mmmh
Stock Number:
039322-MU
Citation ID:
RRID:MMRRC_039322-MU
Other Names:
R1255 (G1), C57BL/6J-MtgxR1255Btlr
Major Collection:

Gene Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231051
Homologene: 46480
Plg
Name: plasminogen
Synonyms: Pg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18815
VEGA: 17
Homologene: 55452
Zfp729a
Name: zinc finger protein 729a
Synonyms: A530054K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 212281
Homologene: 133713
Dnm1l
Name: dynamin 1-like
Synonyms: 6330417M19Rik, Drp1, python, Dnmlp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74006
VEGA: 16
HGNC: HGNC:2973
Homologene: 6384
Kif20b
Name: kinesin family member 20B
Synonyms: N-6 kinesin, C330014J10Rik, Kif20b, Mphosph1, 33cex, magoo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240641
VEGA: 19
HGNC: HGNC:7212
Homologene: 9418
Uhrf1bp1l
Name: UHRF1 (ICBP90) binding protein 1-like
Synonyms: 4930506D01Rik, 2010319N22Rik, E030041M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 75089
VEGA: 10
Homologene: 12590
Rbm28
Name: RNA binding motif protein 28
Synonyms: 2810480G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68272
Homologene: 135952
Atxn3
Name: ataxin 3
Synonyms: Sca3, MJD1, 2210008M02Rik, ataxin-3, Mjd, Atx3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 110616
Homologene: 3658
Gtf2f1
Name: general transcription factor IIF, polypeptide 1
Synonyms: 2810405L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 98053
VEGA: 17
HGNC: HGNC:4652
Homologene: 1585
Son
Name: Son DNA binding protein
Synonyms: 2900011L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 20658
Homologene: 10551
Sema6a
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Synonyms: VIa, sema, Sema6A-1, Semaq, A730020P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 20358
Homologene: 32426
Ebf3
Name: early B cell factor 3
Synonyms: O/E-2, Olf-1/EBF-like 2, 3110018A08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13593
Homologene: 56472
Tln1
Name: talin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21894
Homologene: 21267
Epha5
Name: Eph receptor A5
Synonyms: Cek7, bsk, Els1, Rek7, Hek7, Ehk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13839
HGNC: HGNC:3389
Homologene: 55824
Dennd5b
Name: DENN/MADD domain containing 5B
Synonyms: 9330160C06Rik, D030011O10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320560
Homologene: 44911
Aff3
Name: AF4/FMR2 family, member 3
Synonyms: LAF-4, 3222402O04Rik, Laf4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16764
HGNC: HGNC:6473
Homologene: 1718
Pik3r4
Name: phosphoinositide-3-kinase regulatory subunit 4
Synonyms: Vps15, p150
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 75669
HGNC: HGNC:8982
Homologene: 24678
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: GlcT-1, Epcs21, Ugcgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22234
Homologene: 37763
Antxr2
Name: anthrax toxin receptor 2
Synonyms: cI-35, CMG-2, CMG2, 2310046B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71914
Homologene: 43236
Kcnn3
Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3
Synonyms: small conductance calcium-activated potassium channel 3, SK3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 140493
HGNC: HGNC:6292
Homologene: 20516
Ciz1
Name: CDKN1A interacting zinc finger protein 1
Synonyms: 0610038H21Rik, 2900056O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68379
Homologene: 8112
Ccdc39
Name: coiled-coil domain containing 39
Synonyms: 4921507O14Rik, D3Ertd789e, b2b1735Clo, b2b1304Clo, b2b2025.1Clo, prh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 51938
Homologene: 12149
Snx25
Name: sorting nexin 25
Synonyms: LOC382008, SBBI31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102141
Homologene: 23744
Col22a1
Name: collagen, type XXII, alpha 1
Synonyms: C80743, 2310067L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69700
Homologene: 43567
Abca14
Name: ATP-binding cassette, sub-family A (ABC1), member 14
Synonyms: 1700110B15Rik, 4930539G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67928
Homologene: 86128
Or5ak22
Name: olfactory receptor family 5 subfamily AK member 22
Synonyms: GA_x6K02T2Q125-46877170-46876241, MOR203-1, Olfr992
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258865
Homologene: 121517
Rad51ap2
Name: RAD51 associated protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 209550
VEGA: 12
Homologene: 85245
Acsf3
Name: acyl-CoA synthetase family member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 257633
Homologene: 14958
Tcn2
Name: transcobalamin 2
Synonyms: Tcn-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21452
Homologene: 303
Abcb10
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 10
Synonyms: ABC-me
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 56199
HGNC: HGNC:41
Homologene: 6474
Slc47a1
Name: solute carrier family 47, member 1
Synonyms: 1300013J15Rik, MATE1, mMATE1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67473
Homologene: 34364
Asphd2
Name: aspartate beta-hydroxylase domain containing 2
Synonyms: 9230106G13Rik, 2900006N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72898
Homologene: 10717
Gimap1
Name: GTPase, IMAP family member 1
Synonyms: IAP38, imap, 38kDa, Imap38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16205
Homologene: 7379
Nipal2
Name: NIPA-like domain containing 2
Synonyms: 9330161F08Rik, Npal2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223473
VEGA: 15
Homologene: 41586
Spz1
Name: spermatogenic leucine zipper 1
Synonyms: 1700027M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 79401
VEGA: 13
Homologene: 49934
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 38,204,884 bp
  • T to A, chromosome 2 at 32,365,876 bp
  • T to A, chromosome 2 at 85,400,303 bp
  • T to C, chromosome 3 at 33,826,480 bp
  • A to T, chromosome 3 at 89,652,109 bp
  • T to C, chromosome 4 at 43,538,044 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • A to T, chromosome 5 at 25,351,153 bp
  • G to T, chromosome 5 at 84,150,395 bp
  • C to T, chromosome 5 at 84,150,396 bp
  • A to T, chromosome 5 at 97,975,372 bp
  • A to C, chromosome 5 at 112,391,811 bp
  • C to T, chromosome 6 at 29,158,247 bp
  • T to A, chromosome 6 at 48,743,006 bp
  • A to T, chromosome 6 at 149,041,650 bp
  • A to T, chromosome 7 at 120,207,793 bp
  • C to T, chromosome 7 at 137,225,212 bp
  • A to T, chromosome 8 at 46,116,238 bp
  • T to C, chromosome 8 at 122,785,966 bp
  • C to T, chromosome 8 at 123,962,052 bp
  • T to C, chromosome 9 at 105,653,942 bp
  • T to C, chromosome 10 at 89,745,270 bp
  • T to C, chromosome 11 at 3,922,120 bp
  • A to T, chromosome 11 at 61,370,148 bp
  • G to T, chromosome 12 at 11,458,094 bp
  • T to A, chromosome 12 at 101,934,334 bp
  • T to C, chromosome 13 at 67,621,846 bp
  • C to A, chromosome 13 at 92,575,630 bp
  • T to C, chromosome 15 at 34,584,682 bp
  • C to T, chromosome 15 at 71,846,233 bp
  • G to A, chromosome 16 at 16,316,452 bp
  • T to A, chromosome 16 at 91,664,695 bp
  • T to A, chromosome 17 at 12,384,510 bp
  • A to G, chromosome 17 at 57,010,982 bp
  • A to T, chromosome 18 at 47,249,299 bp
  • A to G, chromosome 19 at 34,950,106 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1255 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039322-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.