Strain Name:
C57BL/6J-MtgxR1425Btlr/Mmmh
Stock Number:
039481-MU
Citation ID:
RRID:MMRRC_039481-MU
Other Names:
R1425 (G1), C57BL/6J-MtgxR1425Btlr
Major Collection:

Gene Information

Rfc1
Name: replication factor C (activator 1) 1
Synonyms: 140kDa, Alp145, RFC140, Recc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19687
HGNC: HGNC:9969
Homologene: 2187
Itgb2
Name: integrin beta 2
Synonyms: Cd18, 2E6, Mac-1 beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16414
HGNC: HGNC:6155
Homologene: 20092
Lama5
Name: laminin, alpha 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16776
HGNC: HGNC:6485
Homologene: 4060
Zfp738
Name: zinc finger protein 738
Synonyms: 3830402I07Rik, 6720487G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408068
Homologene: 133713
Gdpd4
Name: glycerophosphodiester phosphodiesterase domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233537
Homologene: 18606
Sec14l3
Name: SEC14-like lipid binding 3
Synonyms: 1110069O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380683
Homologene: 24848
Olfr690
Name: olfactory receptor 690
Synonyms: GA_x6K02T2PBJ9-7959171-7958224, MOR31-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56860
Homologene: 10653
Kcnt2
Name: potassium channel, subfamily T, member 2
Synonyms: E330038N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240776
Homologene: 16121
Tas2r115
Name: taste receptor, type 2, member 115
Synonyms: T2R15, mt2r49, mGR15, Tas2r15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 353325
Atp6ap1l
Name: ATPase, H+ transporting, lysosomal accessory protein 1-like
Synonyms: EG435376
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 435376
Homologene: 28130
Ppm1k
Name: protein phosphatase 1K (PP2C domain containing)
Synonyms: 2900063A19Rik, PP2Cm, A930026L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243382
Homologene: 36819
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 140,383,028 bp
  • C to T, chromosome 2 at 180,190,811 bp
  • A to C, chromosome 5 at 65,319,518 bp
  • C to T, chromosome 6 at 57,524,789 bp
  • T to G, chromosome 6 at 132,737,479 bp
  • A to T, chromosome 7 at 97,974,012 bp
  • A to T, chromosome 7 at 105,329,715 bp
  • G to A, chromosome 10 at 77,547,296 bp
  • A to G, chromosome 11 at 4,066,487 bp
  • T to C, chromosome 13 at 67,670,775 bp
  • T to C, chromosome 13 at 90,899,519 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1425 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039481-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.