Strain Name:
C57BL/6J-MtgxR1438Btlr/Mmmh
Stock Number:
039493-MU
Citation ID:
RRID:MMRRC_039493-MU
Other Names:
R1438 (G1), C57BL/6J-MtgxR1438Btlr
Major Collection:

Gene Information

Gene Symbol: Wnt3 [MGI:98955] (Mus musculus (mouse))
Name: wingless-type MMTV integration site family, member 3
Synonyms: Wnt-3, Int-4
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Dnajc3 [MGI:107373] (Mus musculus (mouse))
Name: DnaJ heat shock protein family (Hsp40) member C3
Synonyms: Dnajc3, p58IPK, Dnajc3b, Prkri, Dnajc3a, mp58
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Kcnc1 [MGI:96667] (Mus musculus (mouse))
Name: potassium voltage gated channel, Shaw-related subfamily, member 1
Synonyms: NGK2, Kcr2-1, C230009H10Rik, KShIIIB, Shaw, Kv3.1, KV4
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Rorb [MGI:1343464] (Mus musculus (mouse))
Name: RAR-related orphan receptor beta
Synonyms: Rorbeta, Nr1f2, hstp, RZR-beta
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Prtg [MGI:2444710] (Mus musculus (mouse))
Name: protogenin
Synonyms: Igdcc5, A230098A12Rik
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Sec23a [MGI:1349635] (Mus musculus (mouse))
Name: SEC23 homolog A, COPII coat complex component
Synonyms: Sec23r, Msec23
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Parp1 [MGI:1340806] (Mus musculus (mouse))
Name: poly (ADP-ribose) polymerase family, member 1
Synonyms: 5830444G22Rik, PARP, parp-1, sPARP-1, Adprp, Adprt1
Chromosome: 1
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 58,153,178 bp
  • A to G, chromosome 1 at 106,173,412 bp
  • T to C, chromosome 1 at 125,872,356 bp
  • A to T, chromosome 1 at 171,852,118 bp
  • A to G, chromosome 1 at 180,591,242 bp
  • A to G, chromosome 2 at 87,457,992 bp
  • T to C, chromosome 2 at 112,757,701 bp
  • T to A, chromosome 2 at 158,314,137 bp
  • T to C, chromosome 2 at 167,976,609 bp
  • T to A, chromosome 2 at 180,182,800 bp
  • A to C, chromosome 3 at 75,956,133 bp
  • A to G, chromosome 4 at 62,428,523 bp
  • A to G, chromosome 4 at 113,556,111 bp
  • T to A, chromosome 5 at 32,146,985 bp
  • T to A, chromosome 5 at 33,413,884 bp
  • T to A, chromosome 5 at 67,267,291 bp
  • T to C, chromosome 5 at 93,148,428 bp
  • A to G, chromosome 5 at 120,122,896 bp
  • A to G, chromosome 5 at 124,798,945 bp
  • A to T, chromosome 5 at 137,554,609 bp
  • A to T, chromosome 5 at 142,038,323 bp
  • T to A, chromosome 6 at 23,969,423 bp
  • G to A, chromosome 6 at 29,755,483 bp
  • C to A, chromosome 6 at 33,008,568 bp
  • T to A, chromosome 6 at 120,761,472 bp
  • A to T, chromosome 7 at 10,050,135 bp
  • A to G, chromosome 7 at 13,277,218 bp
  • T to A, chromosome 7 at 28,103,733 bp
  • A to T, chromosome 7 at 46,428,267 bp
  • T to C, chromosome 7 at 81,548,873 bp
  • T to A, chromosome 7 at 97,347,497 bp
  • C to T, chromosome 7 at 98,621,406 bp
  • G to A, chromosome 7 at 126,671,891 bp
  • T to C, chromosome 7 at 133,737,340 bp
  • A to T, chromosome 8 at 40,681,392 bp
  • T to C, chromosome 9 at 7,453,705 bp
  • T to C, chromosome 9 at 20,779,957 bp
  • C to A, chromosome 9 at 50,607,672 bp
  • C to T, chromosome 9 at 72,910,750 bp
  • T to C, chromosome 9 at 87,019,660 bp
  • T to C, chromosome 9 at 110,314,055 bp
  • T to C, chromosome 10 at 14,468,841 bp
  • T to C, chromosome 10 at 44,442,128 bp
  • T to A, chromosome 10 at 79,035,288 bp
  • A to G, chromosome 10 at 79,293,857 bp
  • T to C, chromosome 10 at 116,256,204 bp
  • A to T, chromosome 10 at 117,054,992 bp
  • A to T, chromosome 11 at 4,911,427 bp
  • T to A, chromosome 11 at 5,103,332 bp
  • A to T, chromosome 11 at 55,287,811 bp
  • T to G, chromosome 11 at 60,185,395 bp
  • G to T, chromosome 11 at 60,730,314 bp
  • T to C, chromosome 11 at 72,912,945 bp
  • G to A, chromosome 11 at 84,110,011 bp
  • C to A, chromosome 11 at 88,844,751 bp
  • G to T, chromosome 11 at 102,860,042 bp
  • A to T, chromosome 11 at 103,808,251 bp
  • A to T, chromosome 12 at 59,002,010 bp
  • A to T, chromosome 12 at 72,304,413 bp
  • C to T, chromosome 12 at 90,332,135 bp
  • A to G, chromosome 13 at 37,930,605 bp
  • A to G, chromosome 13 at 42,158,120 bp
  • A to T, chromosome 13 at 47,088,171 bp
  • A to T, chromosome 13 at 74,190,179 bp
  • A to T, chromosome 13 at 74,224,868 bp
  • A to T, chromosome 13 at 91,859,852 bp
  • T to A, chromosome 14 at 24,154,605 bp
  • T to C, chromosome 14 at 62,414,321 bp
  • A to G, chromosome 14 at 118,968,106 bp
  • A to G, chromosome 15 at 75,674,398 bp
  • A to G, chromosome 15 at 83,615,026 bp
  • A to T, chromosome 16 at 44,488,746 bp
  • T to A, chromosome 17 at 13,855,390 bp
  • T to A, chromosome 17 at 32,393,535 bp
  • T to C, chromosome 17 at 35,530,560 bp
  • C to T, chromosome 17 at 57,839,140 bp
  • A to G, chromosome 18 at 37,497,962 bp
  • A to G, chromosome 19 at 18,955,053 bp
  • T to A, chromosome 19 at 34,619,169 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1438 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039493-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.