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Strain Name:
C57BL/6J-MtgxR1446Btlr/Mmmh
Stock Number:
039501-MU
Citation ID:
RRID:MMRRC_039501-MU
Other Names:
R1446 (G1), C57BL/6J-MtgxR1446Btlr
Major Collection:

Strain Information

Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Ahcyl2
Name: S-adenosylhomocysteine hydrolase-like 2
Synonyms: 4631427C17Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74340
Homologene: 69337
Aasdh
Name: aminoadipate-semialdehyde dehydrogenase
Synonyms: A230062G08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231326
Homologene: 71711
Map3k4
Name: mitogen-activated protein kinase kinase kinase 4
Synonyms: MTK1, MAPKKK4, D17Rp17, RP17, D17Rp17e, Mekk4, T-associated sex reversal, Tas
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26407
VEGA: 17
HGNC: HGNC:6856
Homologene: 31346
Stk24
Name: serine/threonine kinase 24
Synonyms: 1810013H02Rik, STE20
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 223255
VEGA: 14
Homologene: 20793
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: Ggtb3, 2610509D04Rik, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72145
Homologene: 22855
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 75,143,459 bp
  • T to C, chromosome 1 at 86,546,692 bp
  • T to A, chromosome 2 at 24,706,177 bp
  • A to G, chromosome 2 at 26,423,567 bp
  • A to G, chromosome 2 at 35,306,586 bp
  • A to G, chromosome 2 at 85,721,573 bp
  • A to G, chromosome 2 at 88,973,765 bp
  • A to T, chromosome 2 at 90,118,858 bp
  • G to T, chromosome 2 at 106,783,732 bp
  • A to T, chromosome 2 at 114,197,033 bp
  • A to T, chromosome 2 at 130,248,730 bp
  • A to G, chromosome 2 at 144,373,718 bp
  • T to C, chromosome 3 at 30,650,578 bp
  • T to C, chromosome 3 at 34,057,979 bp
  • T to C, chromosome 3 at 53,654,596 bp
  • T to C, chromosome 3 at 99,187,527 bp
  • C to T, chromosome 3 at 136,064,143 bp
  • C to T, chromosome 4 at 3,604,848 bp
  • T to A, chromosome 4 at 63,224,803 bp
  • T to A, chromosome 4 at 112,118,114 bp
  • A to T, chromosome 4 at 126,882,482 bp
  • A to G, chromosome 4 at 145,173,173 bp
  • A to T, chromosome 5 at 65,348,995 bp
  • C to T, chromosome 5 at 76,886,289 bp
  • A to T, chromosome 5 at 87,578,537 bp
  • G to T, chromosome 5 at 101,851,310 bp
  • G to A, chromosome 5 at 112,757,559 bp
  • T to A, chromosome 5 at 124,789,796 bp
  • A to C, chromosome 5 at 129,873,214 bp
  • A to T, chromosome 5 at 137,389,360 bp
  • A to G, chromosome 5 at 139,393,422 bp
  • A to G, chromosome 5 at 145,101,086 bp
  • T to C, chromosome 6 at 29,891,240 bp
  • G to T, chromosome 6 at 39,102,561 bp
  • C to T, chromosome 6 at 40,571,899 bp
  • T to C, chromosome 6 at 67,309,143 bp
  • T to A, chromosome 6 at 135,209,330 bp
  • T to A, chromosome 7 at 23,866,340 bp
  • T to C, chromosome 7 at 28,268,589 bp
  • G to T, chromosome 7 at 139,238,563 bp
  • C to A, chromosome 7 at 139,238,565 bp
  • C to A, chromosome 8 at 71,648,981 bp
  • T to C, chromosome 8 at 85,007,964 bp
  • T to C, chromosome 8 at 105,888,132 bp
  • T to G, chromosome 9 at 38,403,305 bp
  • A to G, chromosome 9 at 38,733,968 bp
  • A to G, chromosome 9 at 46,242,293 bp
  • A to T, chromosome 9 at 58,015,277 bp
  • T to A, chromosome 9 at 59,668,910 bp
  • A to G, chromosome 9 at 70,607,456 bp
  • T to A, chromosome 9 at 78,367,789 bp
  • T to G, chromosome 10 at 39,522,779 bp
  • C to A, chromosome 10 at 53,062,439 bp
  • A to G, chromosome 10 at 90,511,073 bp
  • C to T, chromosome 10 at 97,568,390 bp
  • A to T, chromosome 11 at 34,819,119 bp
  • A to G, chromosome 11 at 74,083,238 bp
  • G to T, chromosome 11 at 82,928,670 bp
  • A to C, chromosome 11 at 84,878,360 bp
  • G to A, chromosome 11 at 102,914,699 bp
  • A to G, chromosome 11 at 106,790,863 bp
  • G to T, chromosome 12 at 58,978,559 bp
  • A to T, chromosome 13 at 11,738,149 bp
  • T to A, chromosome 13 at 49,655,227 bp
  • T to G, chromosome 13 at 62,173,007 bp
  • G to T, chromosome 14 at 23,311,724 bp
  • A to G, chromosome 14 at 33,012,648 bp
  • T to C, chromosome 14 at 50,533,702 bp
  • A to T, chromosome 14 at 55,770,023 bp
  • A to G, chromosome 14 at 121,308,044 bp
  • A to T, chromosome 15 at 6,652,466 bp
  • A to G, chromosome 15 at 64,135,860 bp
  • G to A, chromosome 15 at 88,932,648 bp
  • C to A, chromosome 16 at 79,078,958 bp
  • T to A, chromosome 17 at 12,256,794 bp
  • A to T, chromosome 17 at 36,119,374 bp
  • T to C, chromosome 17 at 86,139,152 bp
  • G to A, chromosome 19 at 4,287,409 bp
  • T to A, chromosome 19 at 5,306,911 bp
  • G to A, chromosome 19 at 13,103,016 bp
  • A to T, chromosome 19 at 20,373,739 bp
  • G to A, chromosome X at 7,560,363 bp
  • A to T, chromosome X at 109,150,111 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1446 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039501-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.