Strain Name:
C57BL/6J-MtgxR1450Btlr/Mmmh
Stock Number:
039505-MU
Citation ID:
RRID:MMRRC_039505-MU
Other Names:
R1450 (G1), C57BL/6J-MtgxR1450Btlr
Major Collection:

Gene Information

Gene Symbol: Ank2 [MGI:88025] (Mus musculus (mouse))
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-B, Ankyrin-2, Gm4392
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Herc3 [MGI:1921248] (Mus musculus (mouse))
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Epb41l1 [MGI:103010] (Mus musculus (mouse))
Name: erythrocyte membrane protein band 4.1 like 1
Synonyms: 4.1N, Epb4.1l1
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Dst [MGI:104627] (Mus musculus (mouse))
Name: dystonin
Synonyms: ah, 2310001O04Rik, bullous pemphigoid antigen 1, A830042E19Rik, Bpag, Macf2, athetoid, Bpag1, nmf339, nmf203, BPAG1, bullous pemphigoid antigen 1, BPAG1-n
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Synj2 [MGI:1201671] (Mus musculus (mouse))
Name: synaptojanin 2
Synonyms: SJ2
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Mcmbp [MGI:1920977] (Mus musculus (mouse))
Name: minichromosome maintenance complex binding protein
Synonyms: 1110007A13Rik
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Kpna2 [MGI:103561] (Mus musculus (mouse))
Name: karyopherin (importin) alpha 2
Synonyms: m-importin, Importin alpha, importin alpha 1, Rch1, 2410044B12Rik, pendulin, m-importin-alpha-P1
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,188,395 bp
  • T to A, chromosome 1 at 34,212,259 bp
  • T to A, chromosome 1 at 34,556,097 bp
  • G to A, chromosome 1 at 38,813,276 bp
  • T to A, chromosome 1 at 72,873,889 bp
  • A to T, chromosome 1 at 120,476,745 bp
  • A to G, chromosome 1 at 128,307,903 bp
  • A to G, chromosome 1 at 150,652,506 bp
  • A to G, chromosome 1 at 161,971,253 bp
  • T to G, chromosome 1 at 182,747,564 bp
  • A to G, chromosome 2 at 13,360,319 bp
  • A to G, chromosome 2 at 84,702,389 bp
  • T to C, chromosome 2 at 156,511,825 bp
  • C to A, chromosome 3 at 90,432,828 bp
  • T to A, chromosome 3 at 108,542,483 bp
  • T to C, chromosome 3 at 126,957,302 bp
  • C to T, chromosome 3 at 138,424,174 bp
  • C to T, chromosome 3 at 144,434,718 bp
  • T to G, chromosome 3 at 158,187,044 bp
  • A to T, chromosome 4 at 19,395,922 bp
  • G to T, chromosome 4 at 21,874,912 bp
  • T to C, chromosome 4 at 101,136,152 bp
  • A to T, chromosome 4 at 102,601,635 bp
  • T to C, chromosome 4 at 118,934,510 bp
  • T to C, chromosome 4 at 139,468,028 bp
  • T to C, chromosome 5 at 33,365,800 bp
  • G to A, chromosome 5 at 76,262,731 bp
  • A to G, chromosome 5 at 106,918,458 bp
  • A to T, chromosome 5 at 123,864,750 bp
  • C to G, chromosome 6 at 52,260,647 bp
  • G to C, chromosome 6 at 52,260,648 bp
  • A to T, chromosome 6 at 58,876,515 bp
  • A to G, chromosome 6 at 132,755,056 bp
  • T to G, chromosome 7 at 45,907,108 bp
  • C to A, chromosome 7 at 62,463,576 bp
  • A to T, chromosome 7 at 65,647,496 bp
  • A to G, chromosome 7 at 103,564,451 bp
  • A to T, chromosome 7 at 108,755,512 bp
  • A to G, chromosome 7 at 108,925,388 bp
  • G to T, chromosome 7 at 128,715,931 bp
  • T to G, chromosome 8 at 15,945,180 bp
  • T to C, chromosome 8 at 27,447,843 bp
  • T to C, chromosome 8 at 93,987,747 bp
  • C to T, chromosome 8 at 95,871,986 bp
  • T to A, chromosome 9 at 14,316,224 bp
  • A to G, chromosome 9 at 18,409,913 bp
  • A to G, chromosome 9 at 64,911,665 bp
  • G to A, chromosome 9 at 110,633,672 bp
  • A to T, chromosome 10 at 29,147,740 bp
  • T to C, chromosome 10 at 127,504,885 bp
  • A to T, chromosome 11 at 9,430,531 bp
  • C to A, chromosome 11 at 46,017,271 bp
  • T to A, chromosome 11 at 60,495,482 bp
  • T to C, chromosome 11 at 65,927,786 bp
  • T to A, chromosome 11 at 101,421,905 bp
  • A to G, chromosome 11 at 106,997,309 bp
  • C to T, chromosome 12 at 112,173,852 bp
  • T to A, chromosome 13 at 12,193,733 bp
  • A to G, chromosome 13 at 106,947,643 bp
  • A to T, chromosome 14 at 37,044,684 bp
  • T to A, chromosome 14 at 64,028,150 bp
  • T to C, chromosome 14 at 120,944,393 bp
  • A to G, chromosome 15 at 75,158,633 bp
  • T to C, chromosome 16 at 17,856,814 bp
  • A to T, chromosome 16 at 32,407,963 bp
  • C to A, chromosome 17 at 6,027,324 bp
  • A to T, chromosome 17 at 23,175,258 bp
  • T to A, chromosome 17 at 35,141,958 bp
  • T to C, chromosome 17 at 56,257,579 bp
  • T to A, chromosome 18 at 20,409,184 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1450 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039505-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.