Strain Name:
C57BL/6J-MtgxR1467Btlr/Mmmh
Stock Number:
039520-MU
Citation ID:
RRID:MMRRC_039520-MU
Other Names:
R1467 (G1), C57BL/6J-MtgxR1467Btlr
Major Collection:

Gene Information

Gene Symbol: Atg7 [MGI:1921494] (Mus musculus (mouse))
Name: autophagy related 7
Synonyms: Apg7l, 1810013K23Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Espl1 [MGI:2146156] (Mus musculus (mouse))
Name: extra spindle pole bodies 1, separase
Synonyms: PRCE, ESP1, Cerp, SSE, separase, PRCE
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Grm1 [MGI:1351338] (Mus musculus (mouse))
Name: glutamate receptor, metabotropic 1
Synonyms: Grm1, 4930455H15Rik, mGluR1, Gprc1a, rcw, nmf373
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Neurod4 [MGI:108055] (Mus musculus (mouse))
Name: neurogenic differentiation 4
Synonyms: MATH-3, bHLHa4, Math3, Atoh3
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Aldh1l1 [MGI:1340024] (Mus musculus (mouse))
Name: aldehyde dehydrogenase 1 family, member L1
Synonyms: Fthfd, 1810048F20Rik
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Tert [MGI:1202709] (Mus musculus (mouse))
Name: telomerase reverse transcriptase
Synonyms: TR
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Pcif1 [MGI:2443858] (Mus musculus (mouse))
Name: PDX1 C-terminal inhibiting factor 1
Synonyms: 2310022K11Rik, F730014I05Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 13,124,532 bp
  • T to A, chromosome 1 at 32,520,513 bp
  • G to A, chromosome 1 at 60,127,993 bp
  • A to T, chromosome 1 at 64,892,882 bp
  • C to T, chromosome 1 at 66,521,581 bp
  • G to T, chromosome 1 at 74,782,275 bp
  • T to A, chromosome 1 at 115,685,168 bp
  • T to C, chromosome 1 at 135,406,543 bp
  • T to A, chromosome 1 at 150,689,590 bp
  • T to C, chromosome 1 at 160,196,950 bp
  • T to C, chromosome 1 at 163,829,120 bp
  • G to A, chromosome 1 at 175,775,722 bp
  • C to T, chromosome 1 at 195,157,509 bp
  • A to T, chromosome 2 at 26,949,395 bp
  • T to A, chromosome 2 at 29,158,905 bp
  • A to T, chromosome 2 at 40,657,356 bp
  • T to C, chromosome 2 at 52,230,047 bp
  • C to T, chromosome 2 at 62,517,620 bp
  • A to G, chromosome 2 at 66,689,558 bp
  • T to C, chromosome 2 at 88,767,488 bp
  • A to T, chromosome 2 at 91,885,703 bp
  • G to T, chromosome 2 at 112,753,002 bp
  • A to T, chromosome 2 at 120,430,928 bp
  • T to G, chromosome 2 at 126,652,327 bp
  • G to T, chromosome 2 at 144,543,857 bp
  • A to G, chromosome 2 at 158,638,697 bp
  • T to C, chromosome 2 at 164,889,138 bp
  • C to A, chromosome 2 at 170,387,932 bp
  • T to A, chromosome 3 at 37,035,945 bp
  • C to A, chromosome 3 at 87,922,437 bp
  • T to A, chromosome 3 at 93,671,330 bp
  • C to T, chromosome 3 at 96,652,229 bp
  • T to C, chromosome 3 at 107,627,408 bp
  • A to G, chromosome 4 at 49,503,101 bp
  • A to G, chromosome 4 at 58,832,753 bp
  • A to T, chromosome 4 at 143,369,880 bp
  • G to T, chromosome 4 at 154,983,732 bp
  • C to T, chromosome 5 at 11,130,370 bp
  • T to G, chromosome 5 at 87,412,373 bp
  • A to G, chromosome 5 at 111,285,388 bp
  • T to C, chromosome 5 at 115,436,175 bp
  • T to A, chromosome 5 at 123,786,984 bp
  • C to A, chromosome 5 at 129,658,190 bp
  • A to G, chromosome 5 at 151,586,836 bp
  • A to G, chromosome 6 at 90,571,928 bp
  • T to A, chromosome 6 at 114,858,982 bp
  • G to A, chromosome 6 at 123,285,461 bp
  • A to T, chromosome 7 at 3,164,259 bp
  • T to A, chromosome 7 at 19,385,886 bp
  • T to C, chromosome 7 at 29,185,008 bp
  • C to T, chromosome 7 at 43,437,433 bp
  • T to C, chromosome 7 at 65,655,696 bp
  • T to C, chromosome 7 at 86,663,977 bp
  • T to C, chromosome 7 at 89,510,009 bp
  • A to T, chromosome 7 at 120,216,182 bp
  • C to A, chromosome 7 at 120,340,538 bp
  • T to C, chromosome 7 at 128,271,256 bp
  • A to T, chromosome 8 at 3,169,720 bp
  • A to G, chromosome 8 at 35,469,130 bp
  • T to C, chromosome 8 at 70,323,169 bp
  • T to A, chromosome 8 at 105,138,035 bp
  • C to T, chromosome 8 at 109,616,368 bp
  • T to C, chromosome 8 at 123,661,290 bp
  • A to T, chromosome 8 at 125,753,550 bp
  • G to A, chromosome 9 at 119,128,003 bp
  • A to T, chromosome 9 at 119,142,578 bp
  • C to A, chromosome 9 at 124,295,463 bp
  • T to C, chromosome 10 at 10,719,958 bp
  • C to T, chromosome 10 at 40,002,635 bp
  • A to G, chromosome 10 at 77,881,192 bp
  • A to T, chromosome 10 at 79,396,299 bp
  • T to A, chromosome 10 at 92,969,763 bp
  • T to C, chromosome 10 at 130,270,604 bp
  • A to G, chromosome 11 at 7,138,396 bp
  • A to G, chromosome 11 at 20,018,109 bp
  • C to T, chromosome 11 at 33,623,622 bp
  • A to G, chromosome 11 at 48,945,640 bp
  • A to G, chromosome 11 at 54,106,410 bp
  • A to G, chromosome 11 at 54,850,389 bp
  • A to G, chromosome 11 at 74,437,027 bp
  • A to T, chromosome 11 at 79,428,626 bp
  • A to G, chromosome 11 at 96,271,938 bp
  • C to T, chromosome 11 at 98,436,175 bp
  • A to T, chromosome 11 at 101,531,107 bp
  • G to T, chromosome 11 at 107,055,055 bp
  • A to G, chromosome 11 at 120,811,040 bp
  • A to G, chromosome 12 at 51,431,498 bp
  • T to C, chromosome 12 at 79,213,748 bp
  • T to C, chromosome 12 at 83,978,067 bp
  • A to T, chromosome 12 at 85,237,600 bp
  • A to G, chromosome 12 at 85,918,962 bp
  • A to G, chromosome 13 at 38,192,712 bp
  • T to C, chromosome 13 at 54,093,871 bp
  • G to A, chromosome 13 at 57,429,369 bp
  • A to G, chromosome 13 at 73,628,209 bp
  • T to A, chromosome 13 at 95,034,172 bp
  • T to C, chromosome 14 at 29,333,779 bp
  • G to T, chromosome 14 at 34,268,662 bp
  • T to C, chromosome 14 at 64,861,578 bp
  • C to T, chromosome 14 at 79,103,694 bp
  • T to C, chromosome 14 at 103,054,567 bp
  • T to C, chromosome 14 at 123,464,656 bp
  • A to T, chromosome 15 at 75,895,921 bp
  • G to T, chromosome 15 at 77,717,766 bp
  • A to G, chromosome 15 at 101,946,293 bp
  • A to G, chromosome 15 at 102,319,858 bp
  • C to A, chromosome 16 at 14,618,583 bp
  • A to T, chromosome 16 at 25,965,868 bp
  • A to G, chromosome 16 at 78,364,800 bp
  • T to A, chromosome 17 at 22,571,047 bp
  • A to G, chromosome 17 at 46,525,373 bp
  • T to C, chromosome 17 at 66,448,327 bp
  • A to T, chromosome 19 at 5,674,894 bp
  • A to T, chromosome 19 at 5,942,065 bp
  • A to G, chromosome 19 at 25,673,606 bp
  • G to T, chromosome 19 at 44,154,209 bp
Genotype Determination
ES Cell Line
N/A
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
MeSH Terms
    Strain Development
    C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    Donor
    Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
    Primary Reference
    MUTAGENETIX record for R1467 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
    Overall Breeding Performance
    Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    039520-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.