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Strain Name:
C57BL/6J-MtgxR1480Btlr/Mmmh
Stock Number:
039533-MU
Citation ID:
RRID:MMRRC_039533-MU
Other Names:
R1480 (G1), C57BL/6J-MtgxR1480Btlr
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Camkk2
Name: calcium/calmodulin-dependent protein kinase kinase 2, beta
Synonyms: 6330570N16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 207565
HGNC: HGNC:1470
Homologene: 32756
Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20348
Homologene: 36201
Cpe
Name: carboxypeptidase E
Synonyms: carboxypeptidase H, CPH, Cph1, Cph-1, NF-alpha1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12876
HGNC: HGNC:2303
Homologene: 48052
Lias
Name: lipoic acid synthetase
Synonyms: MGC7254, mLip1, 4933425M12Rik, 2900022L22Rik, 7a5ex
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 79464
Homologene: 4997
Gabarap
Name: gamma-aminobutyric acid receptor associated protein
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56486
HGNC: HGNC:4067
Homologene: 134119
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 17,145,557 bp
  • T to A, chromosome 1 at 106,881,707 bp
  • A to T, chromosome 1 at 127,459,979 bp
  • C to T, chromosome 1 at 128,091,862 bp
  • A to G, chromosome 1 at 160,995,319 bp
  • C to T, chromosome 1 at 171,145,801 bp
  • A to G, chromosome 1 at 185,316,068 bp
  • A to G, chromosome 1 at 186,632,823 bp
  • C to T, chromosome 2 at 25,433,397 bp
  • A to G, chromosome 2 at 37,532,639 bp
  • T to A, chromosome 2 at 40,903,389 bp
  • A to G, chromosome 2 at 168,183,534 bp
  • C to A, chromosome 3 at 88,985,052 bp
  • C to T, chromosome 3 at 103,987,434 bp
  • G to A, chromosome 4 at 98,469,582 bp
  • G to A, chromosome 4 at 111,566,717 bp
  • A to C, chromosome 4 at 120,768,724 bp
  • G to T, chromosome 4 at 127,048,612 bp
  • A to G, chromosome 4 at 133,680,389 bp
  • A to G, chromosome 5 at 17,682,031 bp
  • G to A, chromosome 5 at 33,664,597 bp
  • T to A, chromosome 5 at 62,669,129 bp
  • T to A, chromosome 5 at 65,392,291 bp
  • G to A, chromosome 5 at 75,637,317 bp
  • T to C, chromosome 5 at 92,649,044 bp
  • C to T, chromosome 5 at 115,941,939 bp
  • T to C, chromosome 5 at 122,734,278 bp
  • C to A, chromosome 5 at 124,078,826 bp
  • C to T, chromosome 5 at 134,588,983 bp
  • T to C, chromosome 5 at 144,818,313 bp
  • A to G, chromosome 6 at 48,753,030 bp
  • A to T, chromosome 6 at 82,492,530 bp
  • A to G, chromosome 6 at 83,059,452 bp
  • G to A, chromosome 6 at 104,835,864 bp
  • C to A, chromosome 6 at 131,685,294 bp
  • G to A, chromosome 6 at 141,487,574 bp
  • T to G, chromosome 7 at 9,014,956 bp
  • T to A, chromosome 7 at 14,122,911 bp
  • A to G, chromosome 7 at 30,436,023 bp
  • T to C, chromosome 7 at 30,606,886 bp
  • C to T, chromosome 7 at 35,303,338 bp
  • T to C, chromosome 7 at 41,374,919 bp
  • A to G, chromosome 7 at 44,116,854 bp
  • T to A, chromosome 7 at 46,060,913 bp
  • C to T, chromosome 7 at 104,348,244 bp
  • T to C, chromosome 7 at 122,594,642 bp
  • C to T, chromosome 7 at 127,203,778 bp
  • T to A, chromosome 7 at 132,950,016 bp
  • C to T, chromosome 7 at 135,704,538 bp
  • T to A, chromosome 8 at 61,124,326 bp
  • T to A, chromosome 8 at 64,594,935 bp
  • T to C, chromosome 8 at 93,274,154 bp
  • T to C, chromosome 8 at 95,109,887 bp
  • T to C, chromosome 8 at 105,615,548 bp
  • T to G, chromosome 8 at 120,572,394 bp
  • T to C, chromosome 9 at 43,260,054 bp
  • G to T, chromosome 9 at 48,489,716 bp
  • TGGTAAAA to T, chromosome 9 at 53,563,066 bp
  • T to C, chromosome 9 at 65,703,311 bp
  • TCCC to TCC, chromosome 9 at 69,489,754 bp
  • G to T, chromosome 9 at 105,687,244 bp
  • A to G, chromosome 9 at 108,510,081 bp
  • G to A, chromosome 10 at 13,253,792 bp
  • T to C, chromosome 10 at 76,709,918 bp
  • T to C, chromosome 10 at 81,265,655 bp
  • A to T, chromosome 11 at 6,347,827 bp
  • G to A, chromosome 11 at 64,995,294 bp
  • T to A, chromosome 11 at 67,093,545 bp
  • T to C, chromosome 11 at 69,991,725 bp
  • A to T, chromosome 11 at 94,565,137 bp
  • A to G, chromosome 12 at 4,212,171 bp
  • A to G, chromosome 12 at 10,374,886 bp
  • C to A, chromosome 12 at 51,768,522 bp
  • A to T, chromosome 12 at 64,957,221 bp
  • C to T, chromosome 13 at 27,574,072 bp
  • A to T, chromosome 13 at 43,398,628 bp
  • G to A, chromosome 13 at 49,057,232 bp
  • G to A, chromosome 13 at 52,835,496 bp
  • A to T, chromosome 14 at 32,209,628 bp
  • C to T, chromosome 14 at 45,461,787 bp
  • T to C, chromosome 14 at 73,262,602 bp
  • T to C, chromosome 15 at 10,459,951 bp
  • T to C, chromosome 15 at 47,731,929 bp
  • G to A, chromosome 15 at 73,532,846 bp
  • A to T, chromosome 16 at 19,769,867 bp
  • A to G, chromosome 17 at 37,279,745 bp
  • C to T, chromosome 18 at 5,057,345 bp
  • T to C, chromosome 18 at 34,909,223 bp
  • T to C, chromosome 19 at 27,398,910 bp
  • T to C, chromosome 19 at 53,987,771 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1480 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039533-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.