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Strain Name:
C57BL/6J-MtgxR1498Btlr/Mmmh
Stock Number:
039549-MU
Citation ID:
RRID:MMRRC_039549-MU
Other Names:
R1498 (G1), C57BL/6J-MtgxR1498Btlr
Major Collection:

Strain Information

Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Hsp90aa1
Name: heat shock protein 90, alpha (cytosolic), class A member 1
Synonyms: hsp4, Hsp90, Hsp86-1, Hsp89, Hspca
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 15519
Homologene: 68464
Zbtb43
Name: zinc finger and BTB domain containing 43
Synonyms: 1700010E06Rik, Zfp297b
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71834
Homologene: 8514
Bcat1
Name: branched chain aminotransferase 1, cytosolic
Synonyms: Eca39, BCATc, Bcat-1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12035
HGNC: HGNC:976
Homologene: 20320
Morc3
Name: microrchidia 3
Synonyms: 1110051N18Rik, D16Jhu32e, 1110051N18Rik, Zcwcc3
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 338467
Homologene: 32257
Acly
Name: ATP citrate lyase
Synonyms: A730098H14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 104112
HGNC: HGNC:115
Homologene: 854
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 12,848,350 bp
  • T to A, chromosome 1 at 154,205,373 bp
  • A to G, chromosome 2 at 33,462,258 bp
  • A to T, chromosome 2 at 36,787,346 bp
  • A to G, chromosome 2 at 86,538,558 bp
  • A to T, chromosome 2 at 112,092,593 bp
  • T to A, chromosome 3 at 64,556,469 bp
  • T to C, chromosome 3 at 117,662,612 bp
  • A to G, chromosome 4 at 32,810,289 bp
  • G to T, chromosome 4 at 34,805,397 bp
  • A to G, chromosome 4 at 136,239,194 bp
  • A to C, chromosome 4 at 148,581,786 bp
  • T to A, chromosome 5 at 37,323,700 bp
  • A to T, chromosome 5 at 87,850,741 bp
  • A to G, chromosome 5 at 114,970,537 bp
  • G to A, chromosome 5 at 118,065,785 bp
  • G to T, chromosome 6 at 28,426,154 bp
  • A to G, chromosome 6 at 48,931,371 bp
  • C to T, chromosome 6 at 66,539,842 bp
  • A to T, chromosome 6 at 71,893,495 bp
  • T to A, chromosome 6 at 90,072,316 bp
  • G to A, chromosome 6 at 94,627,987 bp
  • A to G, chromosome 6 at 106,783,039 bp
  • A to T, chromosome 6 at 122,077,560 bp
  • A to G, chromosome 6 at 129,771,740 bp
  • A to G, chromosome 6 at 145,019,182 bp
  • A to T, chromosome 7 at 10,748,648 bp
  • T to A, chromosome 7 at 66,302,671 bp
  • GCG to GCGACG, chromosome 7 at 97,579,907 bp
  • T to C, chromosome 7 at 100,546,488 bp
  • T to C, chromosome 7 at 106,790,416 bp
  • A to G, chromosome 7 at 145,268,911 bp
  • T to A, chromosome 8 at 3,512,861 bp
  • C to A, chromosome 8 at 45,025,484 bp
  • A to G, chromosome 8 at 92,359,886 bp
  • G to A, chromosome 8 at 116,954,801 bp
  • A to T, chromosome 9 at 7,446,967 bp
  • C to G, chromosome 9 at 38,219,380 bp
  • C to T, chromosome 9 at 42,041,073 bp
  • G to T, chromosome 9 at 44,701,618 bp
  • T to A, chromosome 9 at 80,307,679 bp
  • A to G, chromosome 10 at 39,532,124 bp
  • T to A, chromosome 10 at 80,895,100 bp
  • T to A, chromosome 10 at 88,477,104 bp
  • T to C, chromosome 10 at 128,482,192 bp
  • A to T, chromosome 11 at 24,164,005 bp
  • C to A, chromosome 11 at 43,438,217 bp
  • A to G, chromosome 11 at 49,059,338 bp
  • G to A, chromosome 11 at 50,857,256 bp
  • A to T, chromosome 11 at 51,070,246 bp
  • A to T, chromosome 11 at 53,869,314 bp
  • A to C, chromosome 11 at 69,520,667 bp
  • G to T, chromosome 11 at 100,188,369 bp
  • A to G, chromosome 11 at 100,483,801 bp
  • G to A, chromosome 11 at 120,815,419 bp
  • A to T, chromosome 12 at 35,934,380 bp
  • A to T, chromosome 12 at 91,366,417 bp
  • A to T, chromosome 12 at 110,695,688 bp
  • T to A, chromosome 13 at 13,650,375 bp
  • T to C, chromosome 13 at 74,372,617 bp
  • C to T, chromosome 13 at 95,646,805 bp
  • T to C, chromosome 14 at 20,509,499 bp
  • T to A, chromosome 14 at 28,302,898 bp
  • A to T, chromosome 14 at 54,876,425 bp
  • C to T, chromosome 14 at 73,560,634 bp
  • T to C, chromosome 14 at 122,616,818 bp
  • T to C, chromosome 15 at 7,190,618 bp
  • CTGAATGAAT to CTGAAT, chromosome 15 at 55,080,176 bp
  • T to A, chromosome 15 at 79,719,021 bp
  • T to A, chromosome 15 at 84,334,631 bp
  • T to C, chromosome 15 at 94,638,361 bp
  • T to C, chromosome 15 at 99,593,247 bp
  • T to C, chromosome 15 at 101,194,010 bp
  • T to C, chromosome 16 at 5,104,765 bp
  • A to T, chromosome 16 at 23,431,220 bp
  • A to G, chromosome 16 at 48,734,941 bp
  • T to A, chromosome 16 at 85,900,102 bp
  • A to T, chromosome 16 at 93,853,855 bp
  • T to C, chromosome 17 at 33,912,253 bp
  • A to T, chromosome 17 at 34,623,101 bp
  • A to G, chromosome 17 at 46,655,710 bp
  • A to G, chromosome 17 at 74,446,413 bp
  • A to G, chromosome 18 at 32,852,968 bp
  • A to G, chromosome 18 at 60,996,692 bp
  • A to G, chromosome 18 at 61,232,860 bp
  • T to C, chromosome 18 at 62,178,933 bp
  • C to T, chromosome 18 at 84,559,773 bp
  • T to C, chromosome 19 at 4,744,246 bp
  • GCAC to GC, chromosome X at 20,929,666 bp
  • G to T, chromosome X at 73,127,655 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1498 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039549-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.


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