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Strain Name:
C57BL/6J-MtgxR1509Btlr/Mmmh
Stock Number:
039556-MU
Citation ID:
RRID:MMRRC_039556-MU
Other Names:
R1509 (G1), C57BL/6J-MtgxR1509Btlr
Major Collection:

Strain Information

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Ide
Name: insulin degrading enzyme
Synonyms: 1300012G03Rik, 4833415K22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15925
HGNC: HGNC:5381
Homologene: 3645
Epha4
Name: Eph receptor A4
Synonyms: Sek, Sek1, Tyro1, Hek8, Cek8, 2900005C20Rik, rb
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13838
HGNC: HGNC:3388
Homologene: 20933
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Map1b
Name: microtubule-associated protein 1B
Synonyms: MAP5, Mtap-5, Mtap5, LC1, Mtap1b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17755
VEGA: 13
HGNC: HGNC:6836
Homologene: 38111
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,347,694 bp
  • A to G, chromosome 1 at 4,348,537 bp
  • T to A, chromosome 1 at 9,553,446 bp
  • T to C, chromosome 1 at 30,977,647 bp
  • T to C, chromosome 1 at 77,380,886 bp
  • T to A, chromosome 1 at 87,021,086 bp
  • A to G, chromosome 1 at 132,456,346 bp
  • T to A, chromosome 2 at 31,314,479 bp
  • G to A, chromosome 2 at 31,654,174 bp
  • A to G, chromosome 2 at 37,219,954 bp
  • A to G, chromosome 2 at 37,786,619 bp
  • T to C, chromosome 2 at 54,733,082 bp
  • A to T, chromosome 2 at 72,364,624 bp
  • A to G, chromosome 2 at 104,441,262 bp
  • A to T, chromosome 2 at 111,218,627 bp
  • A to G, chromosome 2 at 126,553,314 bp
  • A to G, chromosome 2 at 157,233,205 bp
  • A to T, chromosome 3 at 64,716,460 bp
  • A to T, chromosome 3 at 94,774,258 bp
  • T to A, chromosome 4 at 55,007,667 bp
  • A to G, chromosome 4 at 123,000,307 bp
  • C to A, chromosome 4 at 123,684,009 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • C to T, chromosome 4 at 137,511,241 bp
  • T to C, chromosome 4 at 141,475,635 bp
  • T to C, chromosome 4 at 141,475,700 bp
  • T to C, chromosome 5 at 34,829,891 bp
  • G to A, chromosome 5 at 38,540,562 bp
  • C to G, chromosome 5 at 41,819,540 bp
  • T to A, chromosome 5 at 72,699,110 bp
  • C to A, chromosome 5 at 108,188,978 bp
  • T to C, chromosome 5 at 109,215,996 bp
  • C to T, chromosome 5 at 130,229,454 bp
  • A to G, chromosome 6 at 48,438,687 bp
  • T to C, chromosome 6 at 50,043,471 bp
  • T to C, chromosome 6 at 73,027,442 bp
  • T to A, chromosome 6 at 83,156,598 bp
  • C to A, chromosome 7 at 45,495,225 bp
  • A to T, chromosome 7 at 103,741,036 bp
  • G to A, chromosome 7 at 126,963,363 bp
  • G to A, chromosome 7 at 131,074,331 bp
  • C to T, chromosome 7 at 139,027,776 bp
  • G to T, chromosome 8 at 25,165,446 bp
  • A to T, chromosome 8 at 27,153,023 bp
  • T to C, chromosome 8 at 75,054,631 bp
  • G to A, chromosome 8 at 83,719,898 bp
  • G to A, chromosome 8 at 105,138,097 bp
  • A to G, chromosome 8 at 105,461,129 bp
  • T to C, chromosome 8 at 106,083,854 bp
  • G to A, chromosome 8 at 109,640,770 bp
  • A to T, chromosome 8 at 122,307,546 bp
  • A to T, chromosome 9 at 15,010,045 bp
  • A to G, chromosome 9 at 20,801,281 bp
  • T to A, chromosome 9 at 65,371,955 bp
  • T to A, chromosome 9 at 95,228,958 bp
  • T to G, chromosome 9 at 109,067,006 bp
  • T to C, chromosome 10 at 12,455,441 bp
  • T to G, chromosome 10 at 24,028,204 bp
  • T to A, chromosome 10 at 128,043,397 bp
  • C to T, chromosome 11 at 67,938,993 bp
  • A to T, chromosome 11 at 69,747,213 bp
  • T to A, chromosome 11 at 116,347,095 bp
  • C to A, chromosome 11 at 120,701,516 bp
  • G to A, chromosome 12 at 11,279,733 bp
  • G to T, chromosome 12 at 56,858,079 bp
  • T to C, chromosome 13 at 77,192,647 bp
  • T to G, chromosome 13 at 81,669,271 bp
  • G to A, chromosome 13 at 91,771,875 bp
  • T to C, chromosome 13 at 99,431,528 bp
  • A to G, chromosome 13 at 113,368,256 bp
  • C to T, chromosome 13 at 119,466,318 bp
  • C to A, chromosome 14 at 31,151,584 bp
  • T to C, chromosome 14 at 32,173,434 bp
  • T to C, chromosome 14 at 70,678,142 bp
  • A to G, chromosome 15 at 73,116,364 bp
  • A to T, chromosome 15 at 85,086,134 bp
  • A to G, chromosome 15 at 102,407,879 bp
  • A to C, chromosome 16 at 15,731,566 bp
  • T to A, chromosome 16 at 18,875,409 bp
  • C to A, chromosome 16 at 91,503,496 bp
  • T to A, chromosome 16 at 96,426,849 bp
  • A to C, chromosome 17 at 27,631,208 bp
  • T to A, chromosome 17 at 38,417,282 bp
  • A to T, chromosome 17 at 56,271,113 bp
  • C to G, chromosome 17 at 69,387,764 bp
  • A to G, chromosome 18 at 5,583,794 bp
  • A to G, chromosome 18 at 46,524,213 bp
  • A to G, chromosome 19 at 8,803,407 bp
  • C to T, chromosome 19 at 10,964,108 bp
  • T to A, chromosome 19 at 12,999,451 bp
  • A to G, chromosome 19 at 37,285,204 bp
  • A to G, chromosome 19 at 40,557,483 bp
  • A to T, chromosome 19 at 41,882,200 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1509 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039556-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.