MMRRC:039560-MU

T to A, chromosome 1 at 4,912,337 bp
T to C, chromosome 1 at 129,776,423 bp
A to G, chromosome 1 at 133,133,350 bp
T to C, chromosome 1 at 136,156,111 bp
T to A, chromosome 1 at 162,045,382 bp
T to G, chromosome 1 at 195,092,093 bp
A to T, chromosome 2 at 10,440,119 bp
T to G, chromosome 2 at 13,523,414 bp
A to T, chromosome 2 at 52,227,244 bp
A to G, chromosome 2 at 67,511,530 bp
A to T, chromosome 2 at 85,904,671 bp
C to T, chromosome 2 at 86,690,797 bp
A to G, chromosome 2 at 101,642,991 bp
G to A, chromosome 2 at 112,709,197 bp
G to A, chromosome 2 at 120,041,117 bp
G to T, chromosome 2 at 127,061,555 bp
A to T, chromosome 2 at 152,576,165 bp
G to A, chromosome 2 at 156,144,513 bp
A to T, chromosome 2 at 160,904,548 bp
A to G, chromosome 2 at 181,589,189 bp
C to A, chromosome 3 at 33,015,013 bp
A to G, chromosome 3 at 33,839,145 bp
A to G, chromosome 3 at 37,409,502 bp
A to T, chromosome 3 at 59,218,077 bp
A to G, chromosome 3 at 63,963,955 bp
A to G, chromosome 3 at 93,018,625 bp
G to A, chromosome 3 at 106,131,904 bp
A to G, chromosome 3 at 114,097,154 bp
C to T, chromosome 3 at 123,601,455 bp
A to C, chromosome 3 at 124,575,336 bp
T to C, chromosome 4 at 6,408,549 bp
G to T, chromosome 4 at 46,083,549 bp
G to A, chromosome 4 at 47,117,956 bp
A to T, chromosome 4 at 58,100,889 bp
T to C, chromosome 4 at 95,902,058 bp
G to A, chromosome 4 at 108,633,226 bp
A to T, chromosome 4 at 117,265,893 bp
T to C, chromosome 4 at 117,760,935 bp
A to C, chromosome 4 at 118,433,107 bp
G to A, chromosome 4 at 134,124,141 bp
A to G, chromosome 4 at 149,351,578 bp
A to G, chromosome 5 at 31,068,762 bp
T to A, chromosome 5 at 109,293,329 bp
A to T, chromosome 5 at 114,809,273 bp
A to G, chromosome 5 at 137,321,379 bp
A to T, chromosome 5 at 146,170,583 bp
A to T, chromosome 5 at 146,296,378 bp
G to A, chromosome 5 at 149,599,294 bp
A to G, chromosome 6 at 89,933,076 bp
A to T, chromosome 6 at 125,170,992 bp
A to G, chromosome 6 at 129,376,302 bp
T to C, chromosome 6 at 129,872,314 bp
T to A, chromosome 6 at 137,443,594 bp
A to G, chromosome 7 at 24,472,591 bp
A to G, chromosome 7 at 29,070,621 bp
A to T, chromosome 7 at 30,133,296 bp
A to G, chromosome 7 at 43,532,194 bp
G to A, chromosome 7 at 87,037,444 bp
A to G, chromosome 7 at 100,930,862 bp
G to T, chromosome 7 at 102,605,302 bp
C to T, chromosome 7 at 104,106,464 bp
A to C, chromosome 7 at 104,382,689 bp
G to A, chromosome 7 at 105,772,071 bp
CTCTTCTTCTTCTTCTTCTTCTTC to CTCTTCTTCTTCTTCTTCTTC, chromosome 7 at 118,852,449 bp
A to T, chromosome 7 at 120,340,099 bp
A to G, chromosome 7 at 143,005,149 bp
T to C, chromosome 8 at 52,196,198 bp
G to A, chromosome 8 at 84,726,526 bp
T to C, chromosome 8 at 84,978,129 bp
T to C, chromosome 8 at 94,089,844 bp
A to T, chromosome 8 at 105,050,277 bp
T to A, chromosome 9 at 7,103,663 bp
C to A, chromosome 9 at 20,809,609 bp
T to C, chromosome 9 at 50,870,145 bp
T to A, chromosome 9 at 59,403,380 bp
A to G, chromosome 9 at 71,724,590 bp
A to T, chromosome 9 at 96,364,153 bp
G to A, chromosome 9 at 108,728,270 bp
A to G, chromosome 9 at 108,855,836 bp
C to A, chromosome 9 at 118,555,732 bp
T to C, chromosome 9 at 123,964,473 bp
T to A, chromosome 10 at 63,169,368 bp
T to A, chromosome 11 at 6,515,140 bp
A to T, chromosome 11 at 77,046,619 bp
T to A, chromosome 11 at 87,729,431 bp
T to A, chromosome 12 at 84,791,944 bp
T to C, chromosome 12 at 110,954,800 bp
A to G, chromosome 13 at 24,756,632 bp
A to T, chromosome 13 at 81,556,957 bp
A to G, chromosome 13 at 81,593,048 bp
A to T, chromosome 13 at 95,630,010 bp
A to T, chromosome 13 at 100,222,206 bp
A to G, chromosome 13 at 110,400,088 bp
T to C, chromosome 14 at 49,961,101 bp
A to G, chromosome 14 at 50,453,681 bp
G to A, chromosome 14 at 66,143,429 bp
A to G, chromosome 14 at 73,322,084 bp
G to A, chromosome 14 at 103,204,389 bp
A to G, chromosome 15 at 9,361,524 bp
G to T, chromosome 15 at 12,373,829 bp
A to T, chromosome 15 at 35,438,730 bp
G to A, chromosome 15 at 36,545,600 bp
A to G, chromosome 15 at 41,797,474 bp
A to G, chromosome 15 at 68,212,680 bp
A to G, chromosome 15 at 78,262,360 bp
A to G, chromosome 15 at 84,953,336 bp
C to T, chromosome 15 at 101,489,657 bp
A to G, chromosome 16 at 4,115,885 bp
G to T, chromosome 16 at 43,869,100 bp
A to T, chromosome 16 at 44,201,769 bp
A to T, chromosome 17 at 18,063,273 bp
T to C, chromosome 17 at 24,895,776 bp
T to C, chromosome 17 at 30,673,888 bp
G to T, chromosome 17 at 34,847,444 bp
T to A, chromosome 17 at 46,226,980 bp
G to T, chromosome 17 at 46,261,442 bp
A to C, chromosome 17 at 80,371,389 bp
G to T, chromosome 18 at 37,437,058 bp
A to G, chromosome 19 at 5,384,813 bp
G to A, chromosome 19 at 5,569,864 bp
G to A, chromosome 19 at 8,841,145 bp
A to T, chromosome 19 at 12,624,675 bp
A to T, chromosome 19 at 22,986,872 bp
GTCATCATCATCATC to GTCATCATCATCATCATC, chromosome X at 6,082,591 bp
A to G, chromosome Y at 2,116,185 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain GQC Summary

Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice

Littermates of all relevant genotypes.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R1513 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
039560-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Information Expand All